Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 115426
• Name: oriT_pUTI-045-1
• Organism: Staphylococcus saprophyticus strain UTI-045
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP054832 (62616..62855 [+], 240 nt)
• oriT length: 240 nt
• IRs (inverted repeats): 171..178, 183..190 (TACCCATT..AATGGGTA) 154..160, 164..170 (ATCTGGC..GCCAGAT) 55..61, 66..72 (TGTCACA..TGTGACA)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 240 nt

>oriT_pUTI-045-1
GAAATATACACAATAATAGGGGTGGAACACTTTTGTGACAATTTCAATATATTGTGTCACAAAAGTGTGACAAGCACTATATATTGTATCACAAAAGTGTGACAATTTAATTTTTGTGACCCCCATAAGAAAAATGTTTAAAACCCTTGAAATATCTGGCTTCGCCAGATTACCCATTATAAAATGGGTACATATTTCCCTTATGCTCTTACAAATATTTAGCTATATTAGGAGGTAATA

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobP2_HSZ48_RS13335_pUTI-045-1

• ID: 9872
• GenBank: WP_069804897
• Name: mobP2_HSZ48_RS13335_pUTI-045-1
• UniProt ID: _
• Length: 391 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 391 a.a.
• Molecular weight: 47004.62 Da
• Isoelectric Point: 8.7967

>WP_069804897.1 MobP2 family relaxase [Staphylococcus saprophyticus]
MPKIVTMSEFEQPQNKSNYGNYVNYVDREEAKKNEHEVFQYDVYAHYMFDEQKSNSMFNENSNYMNKNEI
EYTKTKFNEAQKNNGIMWKDVISFDNDSLKEAGIFDPDCKYLDEQKMRQATRKAMQKFEKKEGLENNLVW
SSSIHYNTDNIHVHVAAVEKDVTRERGKRKYSTIKEMKSSFANELFDMSGERTKINNFIRDRIVKGIKEQ
NEPDYNTEMKKQLKKIHEEVKDIPKKEWQYNNNIMKHVRPEIDKFTEMYIKNNHGEEFESFKQDLKKQSK
LYKETYGDKSEYKKYEETKMNDLYSRAGNTILKQLKSFNEECNGIKYKEPNKLDNKNIPNYNPKVTSKMQ
IGYALNNTLYNTQRALRNDFQKERNINQYHYEFDKSYQPEQ

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (1-367)

Protein structure

No available structure.

T4CP

t4cp2_HSZ48_RS13285_pUTI-045-1

• ID: 11509
• GenBank: WP_241693130
• Name: t4cp2_HSZ48_RS13285_pUTI-045-1
• UniProt ID: _
• Length: 941 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 941 a.a.
• Molecular weight: 108777.60 Da
• Isoelectric Point: 7.4547

>WP_241693130.1 VirD4-like conjugal transfer protein, CD1115 family [Staphylococcus saprophyticus]
MIPFRNKHNHGYHRKKASFSQNKHLLAHPIGLLALLVVGFIFIMLAANFFINMLNNLIFSGGDIFENMKK
INNEWTSYFFKVRFDLALFYIPFFVLLTVLWGKKVYQIRQKFKSLDNQEKASGRFSTKKEIKQQYKAIAQ
REKQFEGEGGLPVAMFGVYDFATKTKQYLEQRKTVKAEINSNPNLTQDEKYNLKKEKLGQINKEFSNTFF
LKREFTFIDESPTNNIFVGTSRSGKGEIFVLSMIENYSRSSHQPSLVVNDMKGELYSASNETLENRNYHT
EVFNLDQPMESTMSFNLLQLVIEEYQKGDLGEAQEMTKQITHTLTNNEGVEDNEWNLMSAALINAMILAL
CEEALPEHPEKVTLYSVSNMLQTLSVKKFYKQIQIGNQVKVIETSALDEYMERFPSHSPAKTQYATVEAA
PDKMRSSIIGTALKALQPFTTDTIAKLTSHSSLDLNKLGFPSIIDGFSEPDSQFELNVQVKREINIGTEY
DEVEGINIGVNEYGYWQYPFKTILNIDDRIEIVETDEFNKKVKYYFYVTNIDKKGQVTFEAKGDTYSSNV
TIRKFNSFAKPIAIFMVVPDYNSANHGIASILVNQIVFELSKNSQLYTEKQSTHRRVIFHLDEAGNMPQI
PNLSQKVNVSLSRGLRFNFFVQAFSQIKDTYGDAYDAIMDACQNKVFIMATQEQTLKDFSDMIGSQQITV
RSRSGETDSLKSSITENQEERPLLRADELAHLQEGETVVVRNLKRQDNKRRKVMSYPIFNSGKYAMKYRY
QYLADLMDTDKSIIHFRPLLKERCEHRHLQLETTLVDWEKTIENMQAGIEDDSSNEIDDFTQSLNKSMGE
KANANNPEAYKEENNKKPVTRQKKVLTLKAQLSAEEFDRLTRRFTKTIRMYEEDGNNVDRLTLYKLKQYL
KLRVRNNDITEEQKNKSINYVEQLIEEAKAK

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (204-459)

• TraM recognition site of TraD and TraG (TraG-D_C) (616-733)

Protein structure

No available structure.

Host bacterium

• ID: 15859
• GenBank: NZ_CP054832
• Plasmid name: pUTI-045-1
• Incompatibility group: -
• Plasmid size: 74014 bp
• Coordinate of oriT [Strand]: 62616..62855 [+]
• Host baterium: Staphylococcus saprophyticus strain UTI-045

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21