Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 115414
• Name: oriT_105|unnamed
• Organism: Enterobacter cloacae strain 105
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_MK249855 (33088..33193 [+], 106 nt)
• oriT length: 106 nt
• IRs (inverted repeats): 30..35, 41..46 (CCGCCG..CGGCGG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 106 nt

>oriT_105|unnamed
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

oriT secondary structure

Predicted by RNAfold.

Relaxase

traI_H5B58_RS00280_105|unnamed

• ID: 9864
• GenBank: WP_004187323
• Name: traI_H5B58_RS00280_105|unnamed
• UniProt ID: A0A3U8KUL3
• Length: 659 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 659 a.a.
• Molecular weight: 75179.89 Da
• Isoelectric Point: 9.9948

>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (86-334)

Protein structure

• AlphaFold DB: A0A3U8KUL3

T4CP

t4cp2_H5B58_RS00400_105|unnamed

• ID: 11501
• GenBank: WP_004206885
• Name: t4cp2_H5B58_RS00400_105|unnamed
• UniProt ID: _
• Length: 695 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 695 a.a.
• Molecular weight: 79538.91 Da
• Isoelectric Point: 4.8317

>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

Protein domains

No domain identified.

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 35989..61868

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5B58_RS00260	31073..31384	+	312	WP_004187333	hypothetical protein	-
H5B58_RS00265	31517..32053	+	537	WP_004187332	hypothetical protein	-
H5B58_RS00270	32555..32950	-	396	WP_019725163	hypothetical protein	-
H5B58_RS00430	32949..33512	+	564	WP_020277892	plasmid mobilization protein MobA	-
H5B58_RS00280	33499..35478	+	1980	WP_004187323	TraI/MobA(P) family conjugative relaxase	-
H5B58_RS00285	35492..35992	+	501	WP_004187320	DotD/TraH family lipoprotein	-
H5B58_RS00290	35989..36768	+	780	WP_004187315	type IV secretory system conjugative DNA transfer family protein	traI
H5B58_RS00295	36779..37942	+	1164	WP_004187313	plasmid transfer ATPase TraJ	virB11
H5B58_RS00300	37932..38192	+	261	WP_004187310	IcmT/TraK family protein	traK
H5B58_RS00305	38217..39569	+	1353	WP_172684945	toprim domain-containing protein	-
H5B58_RS00310	39532..41508	+	1977	WP_227626110	LPD7 domain-containing protein	-
H5B58_RS00315	41474..41986	+	513	WP_011091071	hypothetical protein	traL
H5B58_RS00320	41987..42184	-	198	WP_004187464	Hha/YmoA family nucleoid-associated regulatory protein	-
H5B58_RS00325	42171..42581	-	411	WP_004187465	H-NS family nucleoid-associated regulatory protein	-
H5B58_RS00330	42580..43362	+	783	WP_015058941	DotI/IcmL family type IV secretion protein	traM
H5B58_RS00335	43371..44522	+	1152	WP_004187471	DotH/IcmK family type IV secretion protein	traN
H5B58_RS00340	44534..45883	+	1350	WP_004187474	conjugal transfer protein TraO	traO
H5B58_RS00345	45895..46599	+	705	WP_015060002	conjugal transfer protein TraP	traP
H5B58_RS00350	46623..47153	+	531	WP_004187478	conjugal transfer protein TraQ	traQ
H5B58_RS00355	47170..47559	+	390	WP_004187479	DUF6750 family protein	traR
H5B58_RS00360	47605..48099	+	495	WP_004187480	hypothetical protein	-
H5B58_RS00365	48096..51146	+	3051	WP_004187482	hypothetical protein	traU
H5B58_RS00370	51143..52351	+	1209	WP_011091082	conjugal transfer protein TraW	traW
H5B58_RS00375	52348..52944	+	597	WP_015060003	hypothetical protein	-
H5B58_RS00380	52937..55132	+	2196	WP_015062834	DotA/TraY family protein	traY
H5B58_RS00385	55134..55787	+	654	WP_015060005	hypothetical protein	-
H5B58_RS00390	55856..56098	+	243	WP_023893611	IncL/M type plasmid replication protein RepC	-
H5B58_RS00395	56382..57449	+	1068	WP_019725043	plasmid replication initiator RepA	-
H5B58_RS00435	58672..58767	+	96	WP_004206884	DinQ-like type I toxin DqlB	-
H5B58_RS00400	58818..60905	-	2088	WP_004206885	conjugal transfer protein TrbC	-
H5B58_RS00405	60918..61868	-	951	WP_004206886	DsbC family protein	trbB

Host bacterium

• ID: 15847
• GenBank: NZ_MK249855
• Plasmid name: 105|unnamed
• Incompatibility group: IncL/M
• Plasmid size: 61881 bp
• Coordinate of oriT [Strand]: 33088..33193 [+]
• Host baterium: Enterobacter cloacae strain 105

Cargo genes

• Drug resistance gene: blaOXA-48
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA8