Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115401 |
| Name | oriT_pR1041-ab1-275k  |
| Organism | Acinetobacter pittii isolate R10-Ab1 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OR095731 (123722..123825 [-], 104 nt) |
| oriT length | 104 nt |
| IRs (inverted repeats) | 63..70, 73..80 (CACCATGC..GCATGGTG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 104 nt
>oriT_pR1041-ab1-275k
GCCCCGCAGGGCAGGATTCCCGTTGAGCGCCGCAGGTGCGAATAAGGGGAAGTGAAGAGGAACACCATGCTTGCATGGTGGGCCTACTTCACACATCCTGCCCG
GCCCCGCAGGGCAGGATTCCCGTTGAGCGCCGCAGGTGCGAATAAGGGGAAGTGAAGAGGAACACCATGCTTGCATGGTGGGCCTACTTCACACATCCTGCCCG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 11491 | GenBank | WP_042892356 |
| Name | t4cp2_RI209_RS00810_pR1041-ab1-275k |
UniProt ID | _ |
| Length | 930 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 930 a.a. Molecular weight: 104210.92 Da Isoelectric Point: 6.1065
>WP_042892356.1 MULTISPECIES: type IV secretion protein DotL [Acinetobacter]
MASTQKYGTQTKFRPSSLKRDTRSGLEKIHLKLTAPDGANYLVGVLGAIAVILFVVPFGGEIFIGIAYYL
LKRYTHPNDYFFEWPFRAPLHSNSLDASTDITSRVKDDVLDNPKAFLEYARTNRDKLQGEGVTYLGRCRE
TGLPVYASNSDDRTHQIVLGTTGSGKTEYMLGNCANQYVQNSGYIFVDAKGDTKAQQDHYRLCNRFGRNE
DLLTINFITSGRNMLRAQTDKMTNSMNQMSNTSSGMLIEFLINLLDDSGGGGGDMWKGRAMAFIAALTRV
LVYLRDNGFIQLSPKVFTQYMELEALEELVFCHNDKYGIDFERVAEQLQGYLVSLPGYSSNPKKLKKQET
KTREQHGYITMQLTKAINDLTFNYGHIFGVEHGGDIDIFDVVLNRRILTVPLPALERSPDSLKMLGKLII
GSIKQMMAGSLGNKMEGLRRAIIDARPTNATTSFKLFLDEWGYIVIVGASVLPAQARSLNFSITFGAQTF
EDIERGSKEEAAATWGNTTVKAIGRTTSGAESTTYKLVDGFAGEEWQGKVNSINMYQGLVFNRSVPQTEV
QFAKEKRVTIEQIAGQHNGEFTLLISTKGEGGKTSDVKIVSMLAFYVAGDQPKYLRLNDLCPIFNIQKSE
IYDPTLKIEDFIHEIEEKHTLLTDNNSSANVTALGDFEICRKLNNTLYENNNTVKDFTQDFVHQILEARR
LESITPEGMAPGQRTVMSSSTAKGEISKEQKHRESIVSAQKEANIKDQIIEKRKQFQYFGKDSDISPQVQ
AGAYNNADLDAEFNIYGERISEPEQAQQESDVETVTRAVITPMNLDSYYDELKEILTGYDLTVAPITEYQ
LVQIPRHVKPEDQTQYIAENKASLDQAISHSTKNEMEFVEQQYAAKFEKALMKNPFSHECVTFEKLLVIL
GSKGSSASLRSGITIKKKDD
MASTQKYGTQTKFRPSSLKRDTRSGLEKIHLKLTAPDGANYLVGVLGAIAVILFVVPFGGEIFIGIAYYL
LKRYTHPNDYFFEWPFRAPLHSNSLDASTDITSRVKDDVLDNPKAFLEYARTNRDKLQGEGVTYLGRCRE
TGLPVYASNSDDRTHQIVLGTTGSGKTEYMLGNCANQYVQNSGYIFVDAKGDTKAQQDHYRLCNRFGRNE
DLLTINFITSGRNMLRAQTDKMTNSMNQMSNTSSGMLIEFLINLLDDSGGGGGDMWKGRAMAFIAALTRV
LVYLRDNGFIQLSPKVFTQYMELEALEELVFCHNDKYGIDFERVAEQLQGYLVSLPGYSSNPKKLKKQET
KTREQHGYITMQLTKAINDLTFNYGHIFGVEHGGDIDIFDVVLNRRILTVPLPALERSPDSLKMLGKLII
GSIKQMMAGSLGNKMEGLRRAIIDARPTNATTSFKLFLDEWGYIVIVGASVLPAQARSLNFSITFGAQTF
EDIERGSKEEAAATWGNTTVKAIGRTTSGAESTTYKLVDGFAGEEWQGKVNSINMYQGLVFNRSVPQTEV
QFAKEKRVTIEQIAGQHNGEFTLLISTKGEGGKTSDVKIVSMLAFYVAGDQPKYLRLNDLCPIFNIQKSE
IYDPTLKIEDFIHEIEEKHTLLTDNNSSANVTALGDFEICRKLNNTLYENNNTVKDFTQDFVHQILEARR
LESITPEGMAPGQRTVMSSSTAKGEISKEQKHRESIVSAQKEANIKDQIIEKRKQFQYFGKDSDISPQVQ
AGAYNNADLDAEFNIYGERISEPEQAQQESDVETVTRAVITPMNLDSYYDELKEILTGYDLTVAPITEYQ
LVQIPRHVKPEDQTQYIAENKASLDQAISHSTKNEMEFVEQQYAAKFEKALMKNPFSHECVTFEKLLVIL
GSKGSSASLRSGITIKKKDD
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 145996..180867
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| RI209_RS00795 (ABCDPFEC_00167) | 141097..142185 | - | 1089 | WP_065995116 | DUF6685 family protein | - |
| RI209_RS00800 (ABCDPFEC_00168) | 142272..142445 | - | 174 | WP_162900306 | hypothetical protein | - |
| RI209_RS00805 | 142840..143040 | + | 201 | WP_078389757 | hypothetical protein | - |
| RI209_RS00810 (ABCDPFEC_00169) | 143081..145873 | - | 2793 | WP_042892356 | type IV secretion protein DotL | - |
| RI209_RS00815 (ABCDPFEC_00170) | 145996..149178 | - | 3183 | WP_120364225 | hypothetical protein | traU |
| RI209_RS00820 (ABCDPFEC_00171) | 149236..150438 | - | 1203 | WP_233735412 | hypothetical protein | - |
| RI209_RS00825 (ABCDPFEC_00172) | 150449..151336 | - | 888 | WP_024160797 | HNH endonuclease | - |
| RI209_RS00830 (ABCDPFEC_00173) | 151447..151917 | - | 471 | WP_309411213 | hypothetical protein | - |
| RI209_RS00835 (ABCDPFEC_00174) | 152028..152603 | - | 576 | WP_024160795 | hypothetical protein | - |
| RI209_RS00840 (ABCDPFEC_00175) | 152614..153495 | - | 882 | WP_024160794 | hypothetical protein | - |
| RI209_RS00845 (ABCDPFEC_00176) | 153501..155012 | - | 1512 | WP_024160793 | DotG/IcmE/VirB10 family protein | - |
| RI209_RS00850 (ABCDPFEC_00177) | 155034..155981 | - | 948 | WP_005005900 | DotH/IcmK family type IV secretion protein | traN |
| RI209_RS00855 (ABCDPFEC_00178) | 155995..156678 | - | 684 | WP_005005897 | DotI/IcmL family type IV secretion protein | traM |
| RI209_RS00860 (ABCDPFEC_00179) | 156689..156976 | - | 288 | WP_024160792 | hypothetical protein | - |
| RI209_RS00865 (ABCDPFEC_00180) | 156977..158206 | - | 1230 | WP_227555133 | hypothetical protein | - |
| RI209_RS00870 (ABCDPFEC_00181) | 159285..159731 | + | 447 | WP_151692142 | DotD/TraH family lipoprotein | - |
| RI209_RS00875 (ABCDPFEC_00182) | 159769..160578 | + | 810 | WP_024160789 | type IV secretory system conjugative DNA transfer family protein | traI |
| RI209_RS00880 (ABCDPFEC_00183) | 160606..161901 | + | 1296 | WP_024160788 | ATPase, T2SS/T4P/T4SS family | virB11 |
| RI209_RS00885 (ABCDPFEC_00185) | 162207..163313 | + | 1107 | WP_233735410 | ParM/StbA family protein | - |
| RI209_RS00890 (ABCDPFEC_00186) | 163310..164137 | + | 828 | WP_233735409 | hypothetical protein | - |
| RI209_RS00895 (ABCDPFEC_00187) | 164182..165243 | - | 1062 | WP_005005876 | ParB/RepB/Spo0J family partition protein | - |
| RI209_RS00900 (ABCDPFEC_00188) | 165253..166089 | - | 837 | WP_032055048 | ParA family protein | - |
| RI209_RS00905 (ABCDPFEC_00189) | 167540..169069 | + | 1530 | WP_227557108 | hypothetical protein | trbA |
| RI209_RS00910 (ABCDPFEC_00190) | 169192..170097 | + | 906 | WP_005005870 | hypothetical protein | - |
| RI209_RS00915 (ABCDPFEC_00191) | 170110..170784 | + | 675 | WP_032055049 | hypothetical protein | - |
| RI209_RS00920 (ABCDPFEC_00192) | 170797..171366 | + | 570 | WP_005005866 | hypothetical protein | - |
| RI209_RS00925 (ABCDPFEC_00193) | 171366..171923 | + | 558 | WP_032055050 | hypothetical protein | - |
| RI209_RS00930 (ABCDPFEC_00194) | 171927..173483 | + | 1557 | WP_032055052 | UvrD-helicase domain-containing protein | - |
| RI209_RS00935 (ABCDPFEC_00195) | 173476..174120 | + | 645 | WP_005028658 | hypothetical protein | - |
| RI209_RS00940 (ABCDPFEC_00196) | 174174..174599 | - | 426 | WP_005005821 | hypothetical protein | - |
| RI209_RS00945 (ABCDPFEC_00197) | 174614..175024 | - | 411 | WP_005005820 | hypothetical protein | - |
| RI209_RS00950 (ABCDPFEC_00198) | 175047..177059 | - | 2013 | WP_032055054 | peptidoglycan DD-metalloendopeptidase family protein | traW |
| RI209_RS00955 (ABCDPFEC_00199) | 177303..177797 | + | 495 | WP_032055055 | hypothetical protein | - |
| RI209_RS00960 (ABCDPFEC_00200) | 177859..180867 | - | 3009 | WP_043041459 | DotA/TraY family protein | traY |
| RI209_RS00965 (ABCDPFEC_00201) | 181134..181547 | + | 414 | WP_005028668 | hypothetical protein | - |
| RI209_RS00970 (ABCDPFEC_00202) | 181597..181998 | + | 402 | WP_005005811 | hypothetical protein | - |
| RI209_RS00975 (ABCDPFEC_00203) | 182047..183903 | - | 1857 | WP_233735335 | hypothetical protein | - |
| RI209_RS00980 (ABCDPFEC_00204) | 184001..184483 | - | 483 | WP_005028671 | hypothetical protein | - |
| RI209_RS00985 (ABCDPFEC_00205) | 184492..185280 | - | 789 | WP_233735334 | hypothetical protein | - |
| RI209_RS00990 (ABCDPFEC_00206) | 185234..185806 | - | 573 | WP_166139325 | hypothetical protein | - |
Host bacterium
| ID | 15834 | GenBank | NZ_OR095731 |
| Plasmid name | pR1041-ab1-275k | Incompatibility group | - |
| Plasmid size | 275647 bp | Coordinate of oriT [Strand] | 123722..123825 [-] |
| Host baterium | Acinetobacter pittii isolate R10-Ab1 |
Cargo genes
| Drug resistance gene | aac(3)-IId, aph(3')-VIa, blaNDM-1, sul2, tet(X3), mph(E), msr(E) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrVA2 |