Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115331
Name   oriT_pEC105227S in_silico
Organism   Enterobacter bugandensis strain E105227
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP110987 (43891..43976 [-], 86 nt)
oriT length   86 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 86 nt

>oriT_pEC105227S
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTAATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9792 GenBank   WP_047625724
Name   Relaxase_OQ483_RS25545_pEC105227S insolico UniProt ID   _
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 102442.66 Da        Isoelectric Point: 8.2753

>WP_047625724.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVKASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKAKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLAGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRGRNQSLREYIADTAIAGLRESPVTDWPSLHQRFAKEGLFLTKE
SGELKVKDGWERERPGVTLSAFGHAFDTDKLIRKLGEFVPPAQDIFTQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYAIARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDSTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGGLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIEMLIGSGEFTWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
SEPHAGSFVSVPADIFERVKPVSRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRMRKARIRIEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNTVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGDNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMRFAEMTNQGSNTEAVRNESGPNWKPPSPL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(51-317)


  Protein structure



No available structure.




Auxiliary protein

ID   5744 GenBank   WP_047625725
Name   WP_047625725_pEC105227S insolico UniProt ID   _
Length   111 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 111 a.a.        Molecular weight: 12662.65 Da        Isoelectric Point: 10.4701

>WP_047625725.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSDKASRRGSDKRQKTVIKTLRFTPDEIEQIEQKAEAVGQSVSAYIRNAAMNRKMISLIDDSFMTELLRL
GRLQKHLFVEGKRVGDKEYSTVLVAITELSTTLRKKLMSND

  Protein domains



No domain identified.



  Protein structure



No available structure.




T4CP

ID   11417 GenBank   WP_065189805
Name   trbC_OQ483_RS25560_pEC105227S insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 87614.86 Da        Isoelectric Point: 6.8660

>WP_065189805.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MNYTPVNQALMKRNAWNSPVLELLRDHLLYAAIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRRFPSLFQYETTQESKGKGIFYLGYRRMNDIGRELWLTIDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IHNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAELYCRAIDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHRLE
GLDSEALEITKYKGPFPYMNYLDEVGAYYTERIAVQATQVRSLDFSIVMMSQDQERIESQTSATNVATLM
QNAGTKVAGKIVSDDKTAKTITNAAGQEARANMVSLQRRDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIEPQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAAKAEPWKIIDTFQQRLANRQEAHNLMTEYDTDHRGREENLWEEALEVIRTTTMAERTI
RYITLNKPESEPTQVSAEEIQLAPDAILRTLTLPQPAYIPPSRYRNEPSISPKLPDSPHPDMEWPE

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(440-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 50099..85053

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OQ483_RS25550 (OQ483_25535) 47115..47318 - 204 WP_133147680 hypothetical protein -
OQ483_RS25555 (OQ483_25540) 47452..47721 - 270 WP_022650007 hypothetical protein -
OQ483_RS25560 (OQ483_25545) 47795..50095 - 2301 WP_065189805 F-type conjugative transfer protein TrbC -
OQ483_RS25565 (OQ483_25550) 50099..51355 - 1257 WP_154299600 thioredoxin fold domain-containing protein trbB
OQ483_RS25570 (OQ483_25555) 51336..52544 - 1209 WP_047625723 hypothetical protein trbA
OQ483_RS25575 (OQ483_25560) 52865..53497 - 633 WP_047625722 plasmid IncI1-type surface exclusion protein ExcA -
OQ483_RS25580 (OQ483_25565) 53559..55718 - 2160 WP_047625721 DotA/TraY family protein traY
OQ483_RS25585 (OQ483_25570) 55776..56318 - 543 WP_154299599 conjugal transfer protein TraX -
OQ483_RS25590 (OQ483_25575) 56315..57517 - 1203 WP_032650382 conjugal transfer protein TraW traW
OQ483_RS25595 (OQ483_25580) 57530..58099 - 570 WP_047625730 hypothetical protein traV
OQ483_RS25600 (OQ483_25585) 58099..61152 - 3054 WP_047625720 conjugal transfer protein traU
OQ483_RS25605 (OQ483_25590) 61239..61964 - 726 WP_065189804 hypothetical protein traT
OQ483_RS25610 (OQ483_25595) 62148..62552 - 405 WP_022649996 DUF6750 family protein traR
OQ483_RS25615 (OQ483_25600) 62587..63111 - 525 WP_047625719 conjugal transfer protein TraQ traQ
OQ483_RS25620 (OQ483_25605) 63111..63884 - 774 WP_123839398 conjugal transfer protein TraP traP
OQ483_RS25625 (OQ483_25610) 63865..65172 - 1308 WP_047625718 conjugal transfer protein TraO traO
OQ483_RS25630 (OQ483_25615) 65172..66152 - 981 WP_047625717 DotH/IcmK family type IV secretion protein traN
OQ483_RS25635 (OQ483_25620) 66163..66780 - 618 WP_179297403 DotI/IcmL family type IV secretion protein traM
OQ483_RS25640 (OQ483_25625) 66870..67220 - 351 WP_022649990 hypothetical protein traL
OQ483_RS25645 (OQ483_25630) 67238..70780 - 3543 WP_309258582 LPD7 domain-containing protein -
OQ483_RS25650 (OQ483_25635) 70852..71139 - 288 WP_022649988 hypothetical protein traK
OQ483_RS25655 (OQ483_25640) 71136..72296 - 1161 WP_229564264 plasmid transfer ATPase TraJ virB11
OQ483_RS25660 (OQ483_25645) 72309..73115 - 807 WP_047625715 type IV secretory system conjugative DNA transfer family protein traI
OQ483_RS25665 (OQ483_25650) 73112..73585 - 474 WP_032650400 DotD/TraH family lipoprotein -
OQ483_RS25670 (OQ483_25655) 73656..74072 - 417 WP_125365859 hypothetical protein -
OQ483_RS25675 (OQ483_25660) 74182..75387 - 1206 WP_047625714 conjugal transfer protein TraF -
OQ483_RS25680 (OQ483_25665) 75493..76329 - 837 WP_049221410 hypothetical protein traE
OQ483_RS25685 (OQ483_25670) 76476..76952 - 477 WP_075205937 hypothetical protein -
OQ483_RS25690 (OQ483_25675) 76972..78132 - 1161 WP_047625727 site-specific integrase -
OQ483_RS25695 (OQ483_25680) 79349..80545 - 1197 WP_052952168 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
OQ483_RS25700 (OQ483_25685) 80542..81216 - 675 WP_047625928 prepilin peptidase -
OQ483_RS25705 (OQ483_25690) 81201..81683 - 483 WP_045261878 lytic transglycosylase domain-containing protein virB1
OQ483_RS25710 (OQ483_25695) 81779..82393 - 615 WP_032650407 type 4 pilus major pilin -
OQ483_RS25715 (OQ483_25700) 82407..83501 - 1095 WP_047625930 type II secretion system F family protein -
OQ483_RS25720 (OQ483_25705) 83494..85053 - 1560 WP_047625931 GspE/PulE family protein virB11
OQ483_RS25725 (OQ483_25710) 85056..85571 - 516 WP_080345948 type IV pilus biogenesis protein PilP -
OQ483_RS25730 (OQ483_25715) 85555..86859 - 1305 WP_047625932 type 4b pilus protein PilO2 -
OQ483_RS25735 (OQ483_25720) 86861..88537 - 1677 WP_047625933 PilN family type IVB pilus formation outer membrane protein -
OQ483_RS25740 (OQ483_25725) 88552..88989 - 438 WP_022649971 type IV pilus biogenesis protein PilM -

Host bacterium

ID   15765 GenBank   NZ_CP110987
Plasmid name   pEC105227S Incompatibility group   -
Plasmid size   95278 bp Coordinate of oriT [Strand]   43891..43976 [-]
Host baterium   Enterobacter bugandensis strain E105227

Cargo genes

Drug resistance gene   aph(6)-Id, aph(3'')-Ib
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA7