Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115324
Name   oriT_pBEE99 in_silico
Organism   Enterococcus faecalis
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NC_013533 (32326..32375 [+], 50 nt)
oriT length   50 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_pBEE99
AATATCGCAACATGGTACCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9787 GenBank   WP_012881074
Name   Relaxase_HS082_RS00160_pBEE99 insolico UniProt ID   D1LHG9
Length   561 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 561 a.a.        Molecular weight: 65402.04 Da        Isoelectric Point: 5.7950

>WP_012881074.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecalis]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKSNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKNS
HKKYTMWETESLYKEKIKSRLDFLLEQSSSINDFLEKAEALNLSVDFSKKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIVERLKENKSVLTLKEVVEKYVEKSEKSKNEFDYQLTIDEWQISHKTTRGYYVNVDFG
FGERGKVFIGAYNVDPLENGQYNIYVKRKDFFYLMNEKNADRNRYMTGETLIKQLRLYNGQTPLKKEPVM
RTIDELVNAINFLAANEIEDTRQLELLEEKLEAAFLEAEETLETLDEKMLELHQLSNLLLENELNGELEL
IQQKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENNLERTRNEINQLHEIQAVQKKETANQNNVKPK
L

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(37-296)


  Protein structure


Source ID Structure
AlphaFold DB D1LHG9


Auxiliary protein

ID   5742 GenBank   WP_002367696
Name   WP_002367696_pBEE99 insolico UniProt ID   Q82YL4
Length   118 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 118 a.a.        Molecular weight: 14042.10 Da        Isoelectric Point: 9.9724

>WP_002367696.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Bacteria]
MENKQKLKRPIQRKIRFSKEENELINRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKEQS

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(64-105)


  Protein structure


Source ID Structure
AlphaFold DB Q82YL4


T4CP

ID   11410 GenBank   WP_002370353
Name   t4cp2_HS082_RS00140_pBEE99 insolico UniProt ID   _
Length   609 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 609 a.a.        Molecular weight: 70061.12 Da        Isoelectric Point: 6.0607

>WP_002370353.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYPKGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWCRYMTDEEAILDKISATDIIDHGEITSELKDETHV

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(144-558)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 8127..29681

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS082_RS00025 (pBEE99p04) 3731..4696 - 966 WP_002405090 hypothetical protein -
HS082_RS00030 (pBEE99p05) 5534..5914 + 381 WP_010815880 hypothetical protein -
HS082_RS00035 (pBEE99p06) 5965..6186 + 222 WP_002406556 hypothetical protein -
HS082_RS00040 (pBEE99p08) 6539..7701 + 1163 WP_146946556 IS3 family transposase -
HS082_RS00045 (pBEE99p09) 8127..12044 + 3918 WP_012881072 LPXTG-anchored aggregation substance PrgB/Asc10 prgB
HS082_RS00050 12106..12470 + 365 Protein_8 pheromone response system RNA-binding regulator PrgU -
HS082_RS00055 (pBEE99p10) 12498..13355 + 858 WP_010815883 pheromone response LPXTG-anchored adhesin PrgC prgC
HS082_RS00060 (pBEE99p11) 13397..14296 + 900 WP_012881073 hypothetical protein -
HS082_RS00065 (pBEE99p12) 14316..14750 + 435 WP_002365857 hypothetical protein -
HS082_RS00070 (pBEE99p13) 14797..15024 + 228 WP_002365858 hypothetical protein -
HS082_RS00075 (pBEE99p14) 15038..15334 + 297 WP_010815885 hypothetical protein -
HS082_RS00080 (pBEE99p15) 15349..16149 + 801 WP_010815886 type IV secretion system protein prgHb
HS082_RS00085 (pBEE99p16) 16151..16504 + 354 WP_002365862 PrgI family mobile element protein prgIc
HS082_RS00090 (pBEE99p17) 16458..18848 + 2391 WP_002365863 VirB4-like conjugal transfer ATPase, CD1110 family virb4
HS082_RS00095 (pBEE99p18) 18860..21373 + 2514 WP_010815887 phage tail tip lysozyme prgK
HS082_RS00100 (pBEE99p20) 22097..24031 + 1935 WP_002367922 group II intron reverse transcriptase/maturase -
HS082_RS00105 (pBEE99p21) 24304..24930 + 627 WP_002386490 hypothetical protein prgL
HS082_RS00110 (pBEE99p22) 24908..25162 + 255 WP_011109622 hypothetical protein -
HS082_RS00115 (pBEE99p23) 25146..25754 + 609 WP_002367686 hypothetical protein -
HS082_RS00120 (pBEE99p24) 25867..26622 + 756 WP_002367687 Fic family protein -
HS082_RS00125 (pBEE99p25) 26639..27097 + 459 WP_002370354 single-stranded DNA-binding protein -
HS082_RS00130 (pBEE99p26) 27143..27325 + 183 WP_002367689 hypothetical protein -
HS082_RS00135 (pBEE99p27) 27367..27852 + 486 WP_002386493 PcfB family protein gbs1365
HS082_RS00140 (pBEE99p28) 27852..29681 + 1830 WP_002370353 VirD4-like conjugal transfer protein, CD1115 family virb4
HS082_RS00145 (pBEE99p29) 29732..31891 + 2160 WP_002370352 ArdC-like ssDNA-binding domain-containing protein -
HS082_RS00150 (pBEE99p30) 31924..32196 + 273 WP_002367930 LtrD -
HS082_RS00155 (pBEE99p31) 32442..32798 + 357 WP_002367696 plasmid mobilization relaxosome protein MobC -
HS082_RS00160 (pBEE99p32) 32799..34484 + 1686 WP_012881074 relaxase/mobilization nuclease domain-containing protein -

Host bacterium

ID   15759 GenBank   NC_013533
Plasmid name   pBEE99 Incompatibility group   -
Plasmid size   80600 bp Coordinate of oriT [Strand]   32326..32375 [+]
Host baterium   Enterococcus faecalis

Cargo genes

Drug resistance gene   -
Virulence gene   prgB/asc10
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA9