Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115317
Name   oriT_pDPT3 in_silico
Organism   Shigella sonnei
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NC_020412 (1873..2048 [+], 176 nt)
oriT length   176 nt
IRs (inverted repeats)      102..107, 121..126  (ACAATC..GATTGT)
Location of nic site      66..67
Conserved sequence flanking the
  nic site    		  ACTGGCATAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 176 nt

>oriT_pDPT3
TGTGACTCCATGCGCGGTGAAAAATCGCATTTTAGCGCGTCACTGGTAGTTGCAAAACTGAACTGGCATAATGCACGGCACATCACGAAGTGCGCACTTATACAATCTCCACTTCGTTTCGATTGTGTGCGGTCTGCGACGCTAAAAGAAAACGGCAAAAAGGCATTACGGCAGAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9785 GenBank   WP_023164745
Name   mobQ_HXH29_RS00020_pDPT3 insolico UniProt ID   A0A2Y6H5D8
Length   739 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 739 a.a.        Molecular weight: 84352.56 Da        Isoelectric Point: 8.2796

>WP_023164745.1 MULTISPECIES: MobQ family relaxase [Enterobacteriaceae]
MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRKRGVDDAVILTPANAPSWCGDRSVLW
NAVEKAEQRRNSQLAREIELAIPREISREAARETVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTT
RAVGETGFAGKVRDWNDRALAETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAM
EKRGMETERGEQNRLINSLNLEIQVSRTQLALRTVQEAQRKRELSDAARRAAEALNLTIPAANASADTLR
EFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASLLSEIPLMTWA
EPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIAPGMAELKEKLSAREAERRREE
AEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAH
YPQSVHDPVNVYLSPGGAVMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPH
HWPDNFYFSDDRRPVASPLPSAHRVDITADTVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREG
GFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAAVHKPEE
MPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(18-213)


  Protein structure


Source ID Structure
AlphaFold DB A0A2Y6H5D8


Host bacterium

ID   15752 GenBank   NC_020412
Plasmid name   pDPT3 Incompatibility group   Col156
Plasmid size   8338 bp Coordinate of oriT [Strand]   1873..2048 [+]
Host baterium   Shigella sonnei

Cargo genes

Drug resistance gene   aph(3')-IIa
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -