Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 115306
• Name: oriT_SAP106A
• Organism: Staphylococcus epidermidis
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NC_013379 (24225..24301 [+], 77 nt)
• oriT length: 77 nt
• IRs (inverted repeats): 42..47, 51..56 (CCTAAG..CTTAGG) 22..29, 34..41 (ATTTTTTC..GAAAAAAT) 1..8, 14..21 (AGTGGCTA..TAGCCACT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 77 nt

>oriT_SAP106A
AGTGGCTAGCAATTAGCCACTATTTTTTCGTTAGAAAAAATCCTAAGGGGCTTAGGGATGTTCCCCAACAAGCTGGC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_HHF84_RS00120_SAP106A

• ID: 9779
• GenBank: WP_012818105
• Name: Relaxase_HHF84_RS00120_SAP106A
• UniProt ID: D2JC84
• Length: 336 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 336 a.a.
• Molecular weight: 38783.65 Da
• Isoelectric Point: 7.2872

>WP_012818105.1 relaxase/mobilization nuclease domain-containing protein [Staphylococcus epidermidis]
MATTKISATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASRELYGKTDGNQGHVIIQSFKSDEVTPE
QCNQLGLELAKKIAPNHQVAVYTHNDTDHIHNHIVINAINLETGKKFNNNKQYLRDLRNLNDEVCLEHGL
SVPEKDTARLRYTQTEKAIADPNTKSTTQYSWKDEIREAIDQSQATNMDEFKDHLNQHGIEIERVTPKSI
TYRHLAEDKKVRGRKLGEDYNKGGIEDGFERQIQRRRQQERDSDTKFQPKRPTSNRDEPTERDTGVTQAD
WDQFAQDTNELERSRQAAESARFADEKARRDREERAREKQASRRIINNDRDHGLEI

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-242)

Protein structure

• AlphaFold DB: D2JC84

Host bacterium

• ID: 15741
• GenBank: NC_013379
• Plasmid name: SAP106A
• Incompatibility group: -
• Plasmid size: 38556 bp
• Coordinate of oriT [Strand]: 24225..24301 [+]
• Host baterium: Staphylococcus epidermidis

Cargo genes

• Drug resistance gene: blaZ
• Virulence gene: -
• Metal resistance gene: arsR
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21