Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115277
Name   oriT_pEC1382-1 in_silico
Organism   Enterobacter kobei strain EC1382
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP096850 (137970..138224 [-], 255 nt)
oriT length   255 nt
IRs (inverted repeats)      153..158, 160..165  (AAAAGT..ACTTTT)
 122..130, 135..143  (TTAAGGCTT..AAGCCTTAA)
 49..55, 60..66  (GAATTTT..AAAATTC)
Location of nic site      78..79
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 255 nt

>oriT_pEC1382-1
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9766 GenBank   WP_309164723
Name   mobH_M2B19_RS25435_pEC1382-1 insolico UniProt ID   _
Length   892 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 892 a.a.        Molecular weight: 100443.38 Da        Isoelectric Point: 4.4685

>WP_309164723.1 MobH family relaxase [Enterobacter kobei]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNP

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure



No available structure.




T4CP

ID   11389 GenBank   WP_001284073
Name   traD_M2B19_RS25440_pEC1382-1 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78123.91 Da        Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

AAA-like domain (AAA_10)

(199-249)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 216206..225446

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2B19_RS25840 (M2B19_25835) 211349..212065 - 717 WP_000838219 zinc finger-like domain-containing protein -
M2B19_RS25845 (M2B19_25840) 212062..212262 - 201 WP_000594930 hypothetical protein -
M2B19_RS25850 (M2B19_25845) 212282..213334 - 1053 WP_001065778 hypothetical protein -
M2B19_RS25855 (M2B19_25850) 213811..214686 + 876 WP_000594611 RepB family plasmid replication initiator protein -
M2B19_RS25860 (M2B19_25855) 215835..216188 + 354 WP_000423602 hypothetical protein -
M2B19_RS25865 (M2B19_25860) 216206..216556 + 351 WP_001447770 type IV conjugative transfer system protein TraL traL
M2B19_RS25870 (M2B19_25865) 216568..217356 + 789 WP_000783153 TraE/TraK family type IV conjugative transfer system protein traE
M2B19_RS25875 (M2B19_25870) 217356..218627 + 1272 WP_000592092 type-F conjugative transfer system secretin TraK traK
M2B19_RS25880 (M2B19_25875) 218629..219090 + 462 WP_000521242 plasmid transfer protein HtdO -
M2B19_RS25885 (M2B19_25880) 219080..220435 + 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
M2B19_RS25890 (M2B19_25885) 220443..220925 + 483 WP_000377632 hypothetical protein -
M2B19_RS25895 (M2B19_25890) 221044..221796 + 753 WP_182911723 protein-disulfide isomerase HtdT -
M2B19_RS25900 (M2B19_25895) 221806..222756 + 951 WP_001022588 IncHI-type conjugal transfer lipoprotein TrhV traV
M2B19_RS25905 (M2B19_25900) 222765..225446 + 2682 WP_000387412 TraC family protein virb4
M2B19_RS25910 (M2B19_25905) 227994..228155 + 162 WP_001371949 hypothetical protein -

Host bacterium

ID   15713 GenBank   NZ_CP096850
Plasmid name   pEC1382-1 Incompatibility group   IncHI2
Plasmid size   373329 bp Coordinate of oriT [Strand]   137970..138224 [-]
Host baterium   Enterobacter kobei strain EC1382

Cargo genes

Drug resistance gene   mcr-9, ant(2'')-Ia, aadA2, qacE, sul1, qnrA1
Virulence gene   vipA/tssB
Metal resistance gene   pcoS, pcoE, ncrC, rcnR/yohL, arsC, arsB, arsR, arsH, terW, terZ, terA, terB, terC, terD, terE, merE, merD, merA, merC, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -