Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115259 |
| Name | oriT_pL9  |
| Organism | Enterococcus faecalis strain L9 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP041776 (37052..37073 [+], 22 nt) |
| oriT length | 22 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 22 nt
>oriT_pL9
TTACTGCGTATAAGTGCGCCCT
TTACTGCGTATAAGTGCGCCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 9754 | GenBank | WP_155282204 |
| Name | mobQ_BMT03_RS13095_pL9 |
UniProt ID | _ |
| Length | 661 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 661 a.a. Molecular weight: 77380.71 Da Isoelectric Point: 9.4335
>WP_155282204.1 MobQ family relaxase [Enterococcus faecalis]
MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTL
WNKMELAEKQVNSQLCREVNVALPIELNNTDQRSLIEEFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKRKKIPKLDENRNPILLENGKIKMVSIKTNDWDRKSLVSEIRKDWADKVNQYLKARNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEEKGVISELKRKNLEIQSYNDILAELDKLENQEKILKQDQN
FTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRLGELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETLFRKQLLNKDQLAEVIYNERVIEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKHID
ELSPLAKHNLKKVVNRNAYLPDSDKIELSKEIEITNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQPQKKRNLDKLIKQTKPKENQSLQRNIPLR
MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTL
WNKMELAEKQVNSQLCREVNVALPIELNNTDQRSLIEEFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKRKKIPKLDENRNPILLENGKIKMVSIKTNDWDRKSLVSEIRKDWADKVNQYLKARNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEEKGVISELKRKNLEIQSYNDILAELDKLENQEKILKQDQN
FTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRLGELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETLFRKQLLNKDQLAEVIYNERVIEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKHID
ELSPLAKHNLKKVVNRNAYLPDSDKIELSKEIEITNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQPQKKRNLDKLIKQTKPKENQSLQRNIPLR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 11375 | GenBank | WP_155282209 |
| Name | t4cp2_BMT03_RS13140_pL9 |
UniProt ID | _ |
| Length | 551 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 551 a.a. Molecular weight: 63087.11 Da Isoelectric Point: 4.7040
>WP_155282209.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLSLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVAEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIEENTTNNDNSELPF
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLSLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVAEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIEENTTNNDNSELPF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 39633..51778
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BMT03_RS13470 | 34864..35187 | - | 324 | Protein_42 | IS6-like element IS1216 family transposase | - |
| BMT03_RS13085 (BMT03_13515) | 35659..36480 | + | 822 | WP_155282202 | replication initiation protein | - |
| BMT03_RS13090 (BMT03_13520) | 36483..36878 | + | 396 | WP_155282203 | hypothetical protein | - |
| BMT03_RS13095 (BMT03_13525) | 37273..39258 | + | 1986 | WP_155282204 | MobQ family relaxase | - |
| BMT03_RS13100 (BMT03_13530) | 39282..39614 | + | 333 | WP_010782375 | CagC family type IV secretion system protein | - |
| BMT03_RS13105 (BMT03_13535) | 39633..40016 | + | 384 | WP_001083514 | glycosyl transferase | trsC |
| BMT03_RS13110 (BMT03_13540) | 40033..40662 | + | 630 | WP_155282205 | hypothetical protein | trsD |
| BMT03_RS13115 (BMT03_13545) | 40673..42634 | + | 1962 | WP_155282206 | VirB4 family type IV secretion system protein | virb4 |
| BMT03_RS13120 (BMT03_13550) | 42649..44001 | + | 1353 | WP_155282207 | conjugal transfer protein TraF | trsF |
| BMT03_RS13125 (BMT03_13555) | 44023..45132 | + | 1110 | WP_155282208 | lysozyme family protein | orf14 |
| BMT03_RS13130 (BMT03_13560) | 45145..45696 | + | 552 | WP_141062720 | hypothetical protein | - |
| BMT03_RS13135 (BMT03_13565) | 45701..46132 | + | 432 | WP_001085135 | hypothetical protein | - |
| BMT03_RS13140 (BMT03_13570) | 46125..47780 | + | 1656 | WP_155282209 | VirD4-like conjugal transfer protein, CD1115 family | - |
| BMT03_RS13145 (BMT03_13575) | 47798..48721 | + | 924 | WP_104659243 | hypothetical protein | traP |
| BMT03_RS13150 (BMT03_13580) | 48721..49665 | + | 945 | WP_104659244 | conjugal transfer protein TrbL family protein | trsL |
| BMT03_RS13155 (BMT03_13585) | 49682..50653 | + | 972 | WP_002425009 | hypothetical protein | - |
| BMT03_RS13160 (BMT03_13590) | 50682..51050 | + | 369 | WP_032495652 | hypothetical protein | - |
| BMT03_RS13165 (BMT03_13595) | 51110..51778 | + | 669 | WP_196246632 | LPXTG cell wall anchor domain-containing protein | prgC |
| BMT03_RS13170 (BMT03_13600) | 51928..52158 | - | 231 | WP_231473164 | hypothetical protein | - |
| BMT03_RS13175 (BMT03_13605) | 52840..52998 | + | 159 | Protein_61 | hypothetical protein | - |
| BMT03_RS13475 | 53021..53332 | - | 312 | Protein_62 | IS6-like element IS1216 family transposase | - |
| BMT03_RS13480 | 53343..53711 | - | 369 | Protein_63 | IS6-like element IS1216 family transposase | - |
| BMT03_RS13185 (BMT03_13615) | 53798..53998 | + | 201 | WP_204193108 | conjugal transfer protein | - |
| BMT03_RS13195 (BMT03_13625) | 54827..56767 | + | 1941 | WP_002405437 | tetracycline resistance ribosomal protection protein Tet(S) | - |
Host bacterium
| ID | 15695 | GenBank | NZ_CP041776 |
| Plasmid name | pL9 | Incompatibility group | - |
| Plasmid size | 57536 bp | Coordinate of oriT [Strand] | 37052..37073 [+] |
| Host baterium | Enterococcus faecalis strain L9 |
Cargo genes
| Drug resistance gene | optrA, fexA, tet(S) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |