Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 115258
• Name: oriT_pL9-A
• Organism: Enterococcus faecalis strain L9
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP041775 (5916..5956 [+], 41 nt)
• oriT length: 41 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 41 nt

>oriT_pL9-A
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_BMT03_RS12875_pL9-A

• ID: 9753
• GenBank: WP_012779614
• Name: mobV_BMT03_RS12875_pL9-A
• UniProt ID: C7C2U1
• Length: 408 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 408 a.a.
• Molecular weight: 48229.55 Da
• Isoelectric Point: 7.0097

>WP_012779614.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSMIVARMQKMKAENLVGIGNHNQRKTKNHSNPDIDTSLSKLNYDLVDRTQNYKTDIENFINENKSTTRA
VRKDAVLVNEWIISSDKDFFDNLTESEIENFFERSKDYFAEKFGEKNIRYATVHLDESTPHMHMGIVPFD
KDNKLSAKRVFNRQALRDVQEELPKYLQDFQFEIARGQKGSERKNLTVPEFKKLKEEELEIKKELQIKKD
ELIAYTKENQIDKKLDITPIKEMEDIEIETDEKTLFGKNKTEIVRQWTGNIILSENDYLKLNKEIKKGKK
TEGRLAAILETDVYQENKELKNELKDQIDKNDKDIDDYNDLVKRYNNLYEENTSLKSQIGDLKEEIKLIY
QSTKRFLKDRISDFKAFKEVFKELADNISNISREKGLDSSFKKEFDRENKKKQTRGIR

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

• AlphaFold DB: C7C2U1

Host bacterium

• ID: 15694
• GenBank: NZ_CP041775
• Plasmid name: pL9-A
• Incompatibility group: -
• Plasmid size: 7759 bp
• Coordinate of oriT [Strand]: 5916..5956 [+]
• Host baterium: Enterococcus faecalis strain L9

Cargo genes

• Drug resistance gene: cfr
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -