Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115240
Name   oriT_pAsmA in_silico
Organism   Aeromonas salmonicida subsp. masoucida strain RZ6S-1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP050188 (104733..104862 [+], 130 nt)
oriT length   130 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 130 nt

>oriT_pAsmA
GTTAACCCGTTTGATCGCGTGATGAATGCGCTAAAAAGTCGAGGCCGTAAGAACGCCCACATTCTGAGCATCTTGCAGCTGGATTGGCCTGCGTCTGAATCGATCATCCATCGCCTGAGCGACTACATCA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9739 GenBank   WP_258312893
Name   mobH_G9457_RS23255_pAsmA insolico UniProt ID   _
Length   1016 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1016 a.a.        Molecular weight: 110666.55 Da        Isoelectric Point: 4.8427

>WP_258312893.1 MobH family relaxase [Aeromonas salmonicida]
MLSVLNKLFGWGGSKGESPQSGRLLPLKDMDEEDIPRYPPFAKGLPVAPIDKILATQAELIEKVRNSLGF
TIEDFNRLVLPVIHRYAAFVHLLPASEAHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPKQRRDNE
PRWRLASCFSGLLHDVGKPLADVSITDKNGVVTWNPYFESLHDWAGRNHVDRYFIRWREKRHKRHEQFSL
LAVDRIIPVATREYLSESGPLIIEAMLEAISGTSVNQPVTKLMLRADQESVSRDLKQNRLNVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLQQGVFIAWRNLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGDRAYYRYWEVLPEMIQEAAGSIKILMLRLESNELVFTTEPPAAVAGEVVGDVADAVIE
LIDPDEASDADDESASGMSESLLSAEQEAMNALAGLGFGDAMEILGGTASDDNATQPAPVVDTTPPAKPA
KGAGGRGGKNQAKKTAPAAPEQQQPEPSPQEIAKSTPPLAVENPLQAIMDVGGGLGGLDFPFDAFVAGTS
MSDEPSQALAPEPKQDVANPDNPDTPNLGLMNQVFPNFMNDESTGDVGGMDLPPAWAIEEIPSFHAYEGE
VGQPGNQFVSEELAGADAPDAKGELLTLMATFGEAGPILERAILPVLEGKATLGEVLCLMKGQAVILYPE
GARALGAPSEILTALFHAQAIVPDPVMSGRKVQDFNGVKAIVLTEQLSSAFIAAIKQAESLLGGYQDAFE
LVASPEVKKTQSKKNRGQRKTSRSESSGEDSHAASPERAQVEEKRVIADAQLIDVSVEPPSPPVPPVDTT
PPPDKVVPRKESRGKEKVDKPIQPAPTTKPAEGTQNLARLPPREQPKPESQQKQARVIDVGNIALPGPDL
PEELSPRSIVKEETVDEALYLPPKMTPEKAIQMLKEMIKNRSGRWLVSAVLEEEGFLTTSDKAFEVIAGE
YTGISKHVLGGTLRRAQRRPLLKQRQGKLYLEVDKS

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4CP

ID   11362 GenBank   WP_106761483
Name   t4cp2_G9457_RS22770_pAsmA insolico UniProt ID   _
Length   649 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 649 a.a.        Molecular weight: 71874.17 Da        Isoelectric Point: 6.5667

>WP_106761483.1 MULTISPECIES: conjugal transfer protein TraG [Pseudomonadota]
MRGGRILWGQIAVVFTIVLVMTWAATQWIAFRLGFQPQLGNPWFELVGLPVYYPPAFFWWWFSFDAYAPA
IFVEGGIIAVSGGFLAIAAAIFMSIIRAREARNVATYGSARWAEDREIRAAGLLGPDGVLLGRYDRDYLR
HDGPEHVLCFAPTRSGKGVGLVVPTLLTWPASAIVHDIKGENWTLTAGFRAKHGRVLLFDPTNARSSAYN
PLLEVRQGEWEVRDVQNIADILVDPEGSLDKRNHWEKTSHSLLVGAILHVLYAEKDKTLAGVANFLSDPR
RPVEATLRAMMDTPHLGEAGLHPVIASSARELLNKSENERSGVLSTAMSFLGLYRDPVVARVTAHCDWRI
ADLVGSRQPVTLYLVVPPSDINRTKPLIRLILNQIGRRLTEELTTSGKRHRLLLMLDEFPALGRLDFFES
ALAFMAGYGIKGFLIAQSLNQIERAYGPNNAILDNCHVRVSFATNDERTAKRVSDALGTATEMRDSTNYA
GHRLAPWLGHLMVSRQETARPLLTPGEIMQLPPTDEIVMVAGTPPIRATKARYFEDARLQERILTPPDLV
AAPLAPSPSADDWSGRVVAAESRSATRGNEAEGDPANAGIRREPELPEHEEIVTPTPSPEQEFEFLDDEP
DVDAAKARAMRQRALLQKS

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(111-555)

  Protein structure



No available structure.



ID   11363 GenBank   WP_011899407
Name   traD_G9457_RS23260_pAsmA insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 68931.34 Da        Isoelectric Point: 6.8339

>WP_011899407.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTYDPLAYEMPWRPNYEMSAVFGWVAASVGALAVQQITDMPPEPFYWMTGICGVMTMARLPKAIKLHLLQ
KHLKGRDLEFISIKELQKKVQASPEDMWLGYGFAWENRHAQRVFEILKRDWTSIVGTESTTSKIVRTIRG
KKKKEVPIGQPWIHGVEPKEEHLMQPLKHTEGHSLIVGTTGSGKTRMFDTLISQAILRGEAVIIIDPKGD
KEMRDNARRACEAMGQAERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEGGGDPFKSFGWQA
LNNIAQGLILAYDRPNLIKLRRFLEGGADGLVIKAVQAYAERVMPDWEAAAAPYLEKAANGSRSKLAVAH
MAFYNNVIQPEHPSSELEGLLNMFKHDATHFSKMVSNLLPIMNMLTSGELGPLLSPDATDLSDERQITDS
AKIINNAQVAYLGLDSLTDNMVGSALGSIFLSDLTAVAGDRYNYGVNNLPVNIFVDEAAEVINDPFIQLL
NKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNVFALRIVDAETQEYIAENLPKTRLKYVMRTQ
GQNTDGSEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGKIVKGRLPILTEK

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(470-597)

AAA-like domain (AAA_10)

(168-257)

  Protein structure



No available structure.



ID   11364 GenBank   WP_219935022
Name   traC_G9457_RS23355_pAsmA insolico UniProt ID   _
Length   814 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 814 a.a.        Molecular weight: 91586.60 Da        Isoelectric Point: 5.6300

>WP_219935022.1 MULTISPECIES: type IV secretion system protein TraC [Aeromonas]
MIVTIKKKLDGALIQEHMRASGVIPVLAYSEDEQVFLMDDQSAGFGFICEPMCGADEKVQERMNGFLNQE
FPAKTTLQFLLFRSPDISQEMYQMLGLRDGFRHGLLSPVIQERINYLQQHTTERIMARTNKGIYDNGLIQ
DLKLFVTCKVPIKSNTPTETELVELDQLKTKVESSLQTVGLRPRTMTAVNYVRIMSTLLNWGPDASWRHD
ASRWETDKPICEQLFDYGTDLEVSKNGIRLGGYHAKVLSAKKLPDIAYFGDAVAYTGDLTGGNSSIKENY
MVVTNVFFPEVESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTVYESLKEGAKPVKISYSLVIF
APTKERVESAATAARSIWRESRFELMEDRFVALPIFLNSLPFCTDRDAVRDLFRYKTMTSEQAAVVLPVF
GEWKGTGTFHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELICSYLSEGAQVWVIDAGKS
YQKMSEMLRGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASSKGLLVEWQISALKQVLSSIWE
EKGTTMKVDDIAERCLEGEDLRLKDVGQQLYAFTSEGSYGKYFSNKNNVSFQNQFTVLELDELQGRKHLR
QVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLYE
NAVGRAIAENSASMYLLGQTEETVESVKRAGRLALSDGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRLI
VGDFQKLLYSTDPVDVNAIDQFVNQGLSVPDAIKAVMQARRQAA

  Protein domains


Predicted by InterproScan.

TraC protein (TraC_F_IV)

(21-255)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraG P-loop domain (P-loop_TraG)

(449-808)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 102812..129105

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9457_RS23250 (G9457_23320) 98982..99596 - 615 WP_073536823 hypothetical protein -
G9457_RS23255 (G9457_23325) 99759..102809 + 3051 WP_258312893 MobH family relaxase -
G9457_RS23260 (G9457_23330) 102812..104677 + 1866 WP_011899407 conjugative transfer system coupling protein TraD virb4
G9457_RS23265 (G9457_23335) 104688..105272 + 585 WP_073536825 hypothetical protein -
G9457_RS23270 (G9457_23340) 105229..105858 + 630 WP_219935014 DUF4400 domain-containing protein tfc7
G9457_RS23275 (G9457_23345) 106004..106393 + 390 WP_073536865 hypothetical protein -
G9457_RS23280 (G9457_23350) 106664..106822 + 159 WP_181023145 hypothetical protein -
G9457_RS23285 (G9457_23355) 106823..107317 - 495 WP_219935015 hypothetical protein -
G9457_RS23290 (G9457_23360) 107294..107587 + 294 WP_219935016 hypothetical protein -
G9457_RS23295 (G9457_23365) 107604..107798 + 195 WP_103859340 hypothetical protein -
G9457_RS23300 (G9457_23370) 107850..107996 - 147 WP_219935017 hypothetical protein -
G9457_RS23305 (G9457_23375) 108163..108633 + 471 WP_219935018 hypothetical protein -
G9457_RS23310 (G9457_23380) 108682..108927 + 246 WP_219935019 hypothetical protein -
G9457_RS23315 (G9457_23385) 109462..109743 + 282 WP_011899398 type IV conjugative transfer system protein TraL traL
G9457_RS23320 (G9457_23390) 109740..110375 + 636 WP_219935020 TraE/TraK family type IV conjugative transfer system protein traE
G9457_RS23325 (G9457_23395) 110359..111264 + 906 WP_219935021 type-F conjugative transfer system secretin TraK traK
G9457_RS23330 (G9457_23400) 111267..112586 + 1320 WP_219935051 TraB/VirB10 family protein traB
G9457_RS23335 (G9457_23405) 112583..113149 + 567 WP_073536829 type IV conjugative transfer system lipoprotein TraV traV
G9457_RS23340 (G9457_23410) 113240..113566 + 327 WP_258236882 TraA family conjugative transfer protein -
G9457_RS23345 (G9457_23415) 113748..119234 + 5487 WP_219939308 Ig-like domain-containing protein -
G9457_RS23350 (G9457_23420) 119391..120116 + 726 WP_232484092 DsbC family protein trbB
G9457_RS23355 (G9457_23425) 120113..122557 + 2445 WP_219935022 type IV secretion system protein TraC virb4
G9457_RS23360 (G9457_23430) 122577..122894 + 318 WP_073536868 hypothetical protein -
G9457_RS23365 (G9457_23435) 122891..123421 + 531 WP_219935023 S26 family signal peptidase -
G9457_RS23370 (G9457_23440) 123384..124649 + 1266 WP_219935024 TrbC family F-type conjugative pilus assembly protein traW
G9457_RS23375 (G9457_23445) 124646..125317 + 672 WP_219935025 EAL domain-containing protein -
G9457_RS23380 (G9457_23450) 125314..126321 + 1008 WP_073545153 TraU family protein traU
G9457_RS23385 (G9457_23455) 126331..129105 + 2775 WP_219935026 conjugal transfer mating pair stabilization protein TraN traN
G9457_RS23390 (G9457_23460) 129153..130115 - 963 WP_219935027 IS1595 family transposase -
G9457_RS23395 (G9457_23465) 130564..130842 + 279 WP_219935028 hypothetical protein -
G9457_RS23400 (G9457_23470) 130858..131160 + 303 WP_219935029 hypothetical protein -
G9457_RS23405 (G9457_23475) 131409..132272 - 864 WP_219935030 hypothetical protein -
G9457_RS23410 (G9457_23480) 132417..133079 - 663 WP_219935031 hypothetical protein -
G9457_RS23415 (G9457_23485) 133368..133730 + 363 WP_073536838 hypothetical protein -

Host bacterium

ID   15675 GenBank   NZ_CP050188
Plasmid name   pAsmA Incompatibility group   -
Plasmid size   252699 bp Coordinate of oriT [Strand]   104733..104862 [+]
Host baterium   Aeromonas salmonicida subsp. masoucida strain RZ6S-1

Cargo genes

Drug resistance gene   floR, formA, tet(A), aac(6')-Ib-cr, blaOXA-1, catB3, catA2, ARR-3, qacE, sul1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -