Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115237
Name   oriT_pA in_silico
Organism   Aeromonas salmonicida subsp. masoucida strain RZ6S-1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP049831 (70458..70587 [+], 130 nt)
oriT length   130 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 130 nt

>oriT_pA
GTTAACCCGTTTGATCGCGTGATGAATGCGCTAAAAAGTCGAGGCCGTAAGAACGCCCACATTCTGAGCATCTTGCAGCTGGATTGGCCTGCGTCTGAATCGATCATCCATCGCCTGAGCGACTACATCA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9737 GenBank   WP_258236879
Name   mobH_G9H43_RS22960_pA insolico UniProt ID   _
Length   987 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 987 a.a.        Molecular weight: 107568.93 Da        Isoelectric Point: 4.7871

>WP_258236879.1 MobH family relaxase [Aeromonas salmonicida]
MDEEDIPRYPPFAKGLPVAPIDKILATQAELIEKVRNSLGFTIEDFNRLVLPVIHRYAAFVHLLPASEAH
HHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPKQRRDNEPRWRLASCFSGLLHDVGKPLADVSITDKN
GVVTWNPYFESLHDWAGRNHVDRYFIRWREKRHKRHEQFSLLAVDRIIPVATREYLSESGPLIIEAMLEA
ISGTSVNQPVTKLMLRADQESVSRDLKQNRLNVDEFSYGVPVERYVFDAIRRLVKTGKWKVNEPGAKVWH
LQQGVFIAWRNLGDLYDLISHDKIPGIPRDPDTLADILIERGFAVPNTVQEKGDRAYYRYWEVLPEMIQE
AAGSIKILMLRLESNELVFTTEPPAAVAGEVVGDVADAVIELIDPDEASDADDESASGMSESLLSAEQEA
MNALAGLGFGDAMEILGGTASDDNATQPAPVVDTTPPAKPAKGAGGRGGKNQAKKTAPAAPEQQQPEPSP
QEIAKSTPPLAVENPLQAIMDVGGGLGGLDFPFDAFVAGTSMSDEPSQALAPEPKQDVANPDNPDTPNLG
LMNQVFPNFMNDESTGDVGGMDLPPAWAIEEIPSFHAYEGEVGQPGNQFVSEELAGADAPDAKGELLTLM
ATFGEAGPILERAILPVLEGKATLGEVLCLMKGQAVILYPEGARALGAPSEILTALFHAQAIVPDPVMSG
RKVQDFNGVKAIVLTEQLSSAFIAAIKQAESLLGGYQDAFELVASPEVKKTQSKKNRGQRKTSRSESSGE
DSHAASPERAQVEEKRVIADAQLIDVSVEPPSPPVPPVDTTPPPDKVVPRKESRGKEKVDKPIQPAPTTK
PAEGTQNLARLPPREQPKPESQQKQARVIDVGNIALPGPDLPEELSPRSIVKEETVDEALYLPPKMTPEK
AIQMLKEMIKNRSGRWLVSAVLEEEGFLTTSDKAFEVIAGEYTGISKHVLGGTLRRAQRRPLLKQRQGKL
YLEVDKS

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(22-328)


  Protein structure



No available structure.




T4CP

ID   11356 GenBank   WP_011899407
Name   traD_G9H43_RS22965_pA insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 68931.34 Da        Isoelectric Point: 6.8339

>WP_011899407.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTYDPLAYEMPWRPNYEMSAVFGWVAASVGALAVQQITDMPPEPFYWMTGICGVMTMARLPKAIKLHLLQ
KHLKGRDLEFISIKELQKKVQASPEDMWLGYGFAWENRHAQRVFEILKRDWTSIVGTESTTSKIVRTIRG
KKKKEVPIGQPWIHGVEPKEEHLMQPLKHTEGHSLIVGTTGSGKTRMFDTLISQAILRGEAVIIIDPKGD
KEMRDNARRACEAMGQAERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEGGGDPFKSFGWQA
LNNIAQGLILAYDRPNLIKLRRFLEGGADGLVIKAVQAYAERVMPDWEAAAAPYLEKAANGSRSKLAVAH
MAFYNNVIQPEHPSSELEGLLNMFKHDATHFSKMVSNLLPIMNMLTSGELGPLLSPDATDLSDERQITDS
AKIINNAQVAYLGLDSLTDNMVGSALGSIFLSDLTAVAGDRYNYGVNNLPVNIFVDEAAEVINDPFIQLL
NKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNVFALRIVDAETQEYIAENLPKTRLKYVMRTQ
GQNTDGSEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGKIVKGRLPILTEK

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(470-597)

AAA-like domain (AAA_10)

(168-257)

  Protein structure



No available structure.



ID   11357 GenBank   WP_219935022
Name   traC_G9H43_RS23060_pA insolico UniProt ID   _
Length   814 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 814 a.a.        Molecular weight: 91586.60 Da        Isoelectric Point: 5.6300

>WP_219935022.1 MULTISPECIES: type IV secretion system protein TraC [Aeromonas]
MIVTIKKKLDGALIQEHMRASGVIPVLAYSEDEQVFLMDDQSAGFGFICEPMCGADEKVQERMNGFLNQE
FPAKTTLQFLLFRSPDISQEMYQMLGLRDGFRHGLLSPVIQERINYLQQHTTERIMARTNKGIYDNGLIQ
DLKLFVTCKVPIKSNTPTETELVELDQLKTKVESSLQTVGLRPRTMTAVNYVRIMSTLLNWGPDASWRHD
ASRWETDKPICEQLFDYGTDLEVSKNGIRLGGYHAKVLSAKKLPDIAYFGDAVAYTGDLTGGNSSIKENY
MVVTNVFFPEVESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTVYESLKEGAKPVKISYSLVIF
APTKERVESAATAARSIWRESRFELMEDRFVALPIFLNSLPFCTDRDAVRDLFRYKTMTSEQAAVVLPVF
GEWKGTGTFHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELICSYLSEGAQVWVIDAGKS
YQKMSEMLRGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASSKGLLVEWQISALKQVLSSIWE
EKGTTMKVDDIAERCLEGEDLRLKDVGQQLYAFTSEGSYGKYFSNKNNVSFQNQFTVLELDELQGRKHLR
QVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLYE
NAVGRAIAENSASMYLLGQTEETVESVKRAGRLALSDGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRLI
VGDFQKLLYSTDPVDVNAIDQFVNQGLSVPDAIKAVMQARRQAA

  Protein domains


Predicted by InterproScan.

TraC protein (TraC_F_IV)

(21-255)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraG P-loop domain (P-loop_TraG)

(449-808)

  Protein structure



No available structure.



ID   11358 GenBank   WP_106761483
Name   t4cp2_G9H43_RS23710_pA insolico UniProt ID   _
Length   649 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 649 a.a.        Molecular weight: 71874.17 Da        Isoelectric Point: 6.5667

>WP_106761483.1 MULTISPECIES: conjugal transfer protein TraG [Pseudomonadota]
MRGGRILWGQIAVVFTIVLVMTWAATQWIAFRLGFQPQLGNPWFELVGLPVYYPPAFFWWWFSFDAYAPA
IFVEGGIIAVSGGFLAIAAAIFMSIIRAREARNVATYGSARWAEDREIRAAGLLGPDGVLLGRYDRDYLR
HDGPEHVLCFAPTRSGKGVGLVVPTLLTWPASAIVHDIKGENWTLTAGFRAKHGRVLLFDPTNARSSAYN
PLLEVRQGEWEVRDVQNIADILVDPEGSLDKRNHWEKTSHSLLVGAILHVLYAEKDKTLAGVANFLSDPR
RPVEATLRAMMDTPHLGEAGLHPVIASSARELLNKSENERSGVLSTAMSFLGLYRDPVVARVTAHCDWRI
ADLVGSRQPVTLYLVVPPSDINRTKPLIRLILNQIGRRLTEELTTSGKRHRLLLMLDEFPALGRLDFFES
ALAFMAGYGIKGFLIAQSLNQIERAYGPNNAILDNCHVRVSFATNDERTAKRVSDALGTATEMRDSTNYA
GHRLAPWLGHLMVSRQETARPLLTPGEIMQLPPTDEIVMVAGTPPIRATKARYFEDARLQERILTPPDLV
AAPLAPSPSADDWSGRVVAAESRSATRGNEAEGDPANAGIRREPELPEHEEIVTPTPSPEQEFEFLDDEP
DVDAAKARAMRQRALLQKS

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(111-555)

  Protein structure



No available structure.



ID   11359 GenBank   WP_106761483
Name   t4cp2_G9H43_RS23755_pA insolico UniProt ID   _
Length   649 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 649 a.a.        Molecular weight: 71874.17 Da        Isoelectric Point: 6.5667

>WP_106761483.1 MULTISPECIES: conjugal transfer protein TraG [Pseudomonadota]
MRGGRILWGQIAVVFTIVLVMTWAATQWIAFRLGFQPQLGNPWFELVGLPVYYPPAFFWWWFSFDAYAPA
IFVEGGIIAVSGGFLAIAAAIFMSIIRAREARNVATYGSARWAEDREIRAAGLLGPDGVLLGRYDRDYLR
HDGPEHVLCFAPTRSGKGVGLVVPTLLTWPASAIVHDIKGENWTLTAGFRAKHGRVLLFDPTNARSSAYN
PLLEVRQGEWEVRDVQNIADILVDPEGSLDKRNHWEKTSHSLLVGAILHVLYAEKDKTLAGVANFLSDPR
RPVEATLRAMMDTPHLGEAGLHPVIASSARELLNKSENERSGVLSTAMSFLGLYRDPVVARVTAHCDWRI
ADLVGSRQPVTLYLVVPPSDINRTKPLIRLILNQIGRRLTEELTTSGKRHRLLLMLDEFPALGRLDFFES
ALAFMAGYGIKGFLIAQSLNQIERAYGPNNAILDNCHVRVSFATNDERTAKRVSDALGTATEMRDSTNYA
GHRLAPWLGHLMVSRQETARPLLTPGEIMQLPPTDEIVMVAGTPPIRATKARYFEDARLQERILTPPDLV
AAPLAPSPSADDWSGRVVAAESRSATRGNEAEGDPANAGIRREPELPEHEEIVTPTPSPEQEFEFLDDEP
DVDAAKARAMRQRALLQKS

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(111-555)

  Protein structure



No available structure.



ID   11360 GenBank   WP_106761483
Name   t4cp2_G9H43_RS23800_pA insolico UniProt ID   _
Length   649 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 649 a.a.        Molecular weight: 71874.17 Da        Isoelectric Point: 6.5667

>WP_106761483.1 MULTISPECIES: conjugal transfer protein TraG [Pseudomonadota]
MRGGRILWGQIAVVFTIVLVMTWAATQWIAFRLGFQPQLGNPWFELVGLPVYYPPAFFWWWFSFDAYAPA
IFVEGGIIAVSGGFLAIAAAIFMSIIRAREARNVATYGSARWAEDREIRAAGLLGPDGVLLGRYDRDYLR
HDGPEHVLCFAPTRSGKGVGLVVPTLLTWPASAIVHDIKGENWTLTAGFRAKHGRVLLFDPTNARSSAYN
PLLEVRQGEWEVRDVQNIADILVDPEGSLDKRNHWEKTSHSLLVGAILHVLYAEKDKTLAGVANFLSDPR
RPVEATLRAMMDTPHLGEAGLHPVIASSARELLNKSENERSGVLSTAMSFLGLYRDPVVARVTAHCDWRI
ADLVGSRQPVTLYLVVPPSDINRTKPLIRLILNQIGRRLTEELTTSGKRHRLLLMLDEFPALGRLDFFES
ALAFMAGYGIKGFLIAQSLNQIERAYGPNNAILDNCHVRVSFATNDERTAKRVSDALGTATEMRDSTNYA
GHRLAPWLGHLMVSRQETARPLLTPGEIMQLPPTDEIVMVAGTPPIRATKARYFEDARLQERILTPPDLV
AAPLAPSPSADDWSGRVVAAESRSATRGNEAEGDPANAGIRREPELPEHEEIVTPTPSPEQEFEFLDDEP
DVDAAKARAMRQRALLQKS

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(111-555)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 68537..94829

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9H43_RS22955 (G9H43_23040) 64707..65321 - 615 WP_073536823 hypothetical protein -
G9H43_RS22960 (G9H43_23045) 65571..68534 + 2964 WP_258236879 MobH family relaxase -
G9H43_RS22965 (G9H43_23050) 68537..70402 + 1866 WP_011899407 conjugative transfer system coupling protein TraD virb4
G9H43_RS22970 (G9H43_23055) 70413..70997 + 585 WP_073536825 hypothetical protein -
G9H43_RS22975 (G9H43_23060) 70954..71583 + 630 WP_219935014 DUF4400 domain-containing protein tfc7
G9H43_RS22980 (G9H43_23065) 71729..72118 + 390 WP_073536865 hypothetical protein -
G9H43_RS22985 (G9H43_23070) 72389..72547 + 159 WP_181023145 hypothetical protein -
G9H43_RS22990 (G9H43_23075) 72548..73042 - 495 WP_219935015 hypothetical protein -
G9H43_RS22995 (G9H43_23080) 73019..73312 + 294 WP_219935016 hypothetical protein -
G9H43_RS23000 (G9H43_23085) 73329..73523 + 195 WP_103859340 hypothetical protein -
G9H43_RS23005 (G9H43_23090) 73575..73721 - 147 WP_219935017 hypothetical protein -
G9H43_RS23010 (G9H43_23095) 73888..74358 + 471 WP_219935018 hypothetical protein -
G9H43_RS23015 (G9H43_23100) 74407..74652 + 246 WP_219935019 hypothetical protein -
G9H43_RS23020 (G9H43_23105) 75187..75468 + 282 WP_011899398 type IV conjugative transfer system protein TraL traL
G9H43_RS23025 (G9H43_23110) 75465..76100 + 636 WP_219935020 TraE/TraK family type IV conjugative transfer system protein traE
G9H43_RS23030 (G9H43_23115) 76084..76989 + 906 WP_219935021 type-F conjugative transfer system secretin TraK traK
G9H43_RS23035 (G9H43_23120) 76992..78311 + 1320 WP_219935051 TraB/VirB10 family protein traB
G9H43_RS23040 (G9H43_23125) 78308..78874 + 567 WP_073536829 type IV conjugative transfer system lipoprotein TraV traV
G9H43_RS23045 (G9H43_23130) 78965..79291 + 327 WP_258236882 TraA family conjugative transfer protein -
G9H43_RS23050 (G9H43_23135) 79473..84958 + 5486 Protein_83 Ig-like domain-containing protein -
G9H43_RS23055 (G9H43_23140) 85115..85840 + 726 WP_232484092 DsbC family protein trbB
G9H43_RS23060 (G9H43_23145) 85837..88281 + 2445 WP_219935022 type IV secretion system protein TraC virb4
G9H43_RS23065 (G9H43_23150) 88301..88618 + 318 WP_073536868 hypothetical protein -
G9H43_RS23070 (G9H43_23155) 88615..89145 + 531 WP_219935023 S26 family signal peptidase -
G9H43_RS23075 (G9H43_23160) 89108..90373 + 1266 WP_219935024 TrbC family F-type conjugative pilus assembly protein traW
G9H43_RS23080 (G9H43_23165) 90370..91041 + 672 WP_219935025 EAL domain-containing protein -
G9H43_RS23085 (G9H43_23170) 91038..92045 + 1008 WP_073545153 TraU family protein traU
G9H43_RS23090 (G9H43_23175) 92055..94829 + 2775 WP_219935026 conjugal transfer mating pair stabilization protein TraN traN
G9H43_RS23095 (G9H43_23180) 94877..95839 - 963 WP_219935027 IS1595 family transposase -
G9H43_RS23100 (G9H43_23185) 96288..96566 + 279 WP_219935028 hypothetical protein -
G9H43_RS23105 (G9H43_23190) 96582..96884 + 303 WP_219935029 hypothetical protein -
G9H43_RS23110 (G9H43_23195) 97133..97996 - 864 WP_219935030 hypothetical protein -
G9H43_RS23115 (G9H43_23200) 98141..98803 - 663 WP_219935031 hypothetical protein -
G9H43_RS23120 (G9H43_23205) 99092..99454 + 363 WP_073536838 hypothetical protein -

Host bacterium

ID   15672 GenBank   NZ_CP049831
Plasmid name   pA Incompatibility group   -
Plasmid size   286000 bp Coordinate of oriT [Strand]   70458..70587 [+]
Host baterium   Aeromonas salmonicida subsp. masoucida strain RZ6S-1

Cargo genes

Drug resistance gene   tet(A), aac(6')-Ib-cr, blaOXA-1, catB3, catA2, ARR-3, qacE, sul1, floR, formA
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -