Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115220
Name   oriT_pC-CRECL423 in_silico
Organism   Enterobacter hormaechei strain CRECL423
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP133339 (48512..48662 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_pC-CRECL423
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9726 GenBank   WP_308982204
Name   mobH_RCR42_RS25820_pC-CRECL423 insolico UniProt ID   _
Length   990 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109482.12 Da        Isoelectric Point: 4.9964

>WP_308982204.1 MobH family relaxase [Enterobacter hormaechei]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETTSTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPRLDANKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQPKEKKVDVTSPVEEPQRK
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4CP

ID   11343 GenBank   WP_000178857
Name   traD_RCR42_RS25825_pC-CRECL423 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69225.71 Da        Isoelectric Point: 6.9403

>WP_000178857.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKVKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-299)

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

  Protein structure



No available structure.



ID   11344 GenBank   WP_088212670
Name   traC_RCR42_RS25900_pC-CRECL423 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92632.91 Da        Isoelectric Point: 5.9216

>WP_088212670.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYVGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSQGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRRQAA

  Protein domains


Predicted by InterproScan.

TraC protein (TraC_F_IV)

(21-255)

Family of unknown function (DUF5934) (DUF5934)

(283-434)

TraG P-loop domain (P-loop_TraG)

(449-809)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 46596..71742

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RCR42_RS25810 (RCR42_25810) 42627..42860 - 234 WP_001191890 hypothetical protein -
RCR42_RS25815 (RCR42_25815) 42842..43459 - 618 WP_001249395 hypothetical protein -
RCR42_RS25820 (RCR42_25820) 43627..46599 + 2973 WP_308982204 MobH family relaxase -
RCR42_RS25825 (RCR42_25825) 46596..48461 + 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
RCR42_RS25830 (RCR42_25830) 48511..49056 + 546 WP_247750980 hypothetical protein -
RCR42_RS25835 (RCR42_25835) 49013..49642 + 630 WP_088212662 DUF4400 domain-containing protein tfc7
RCR42_RS25840 (RCR42_25840) 49652..50098 + 447 WP_000122507 hypothetical protein -
RCR42_RS25845 (RCR42_25845) 50108..50485 + 378 WP_088212663 hypothetical protein -
RCR42_RS25850 (RCR42_25850) 50485..51147 + 663 WP_308982208 hypothetical protein -
RCR42_RS25855 (RCR42_25855) 51483..51848 + 366 WP_001052531 hypothetical protein -
RCR42_RS25860 (RCR42_25860) 51993..52274 + 282 WP_088212665 type IV conjugative transfer system protein TraL traL
RCR42_RS25865 (RCR42_25865) 52271..52897 + 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
RCR42_RS25870 (RCR42_25870) 52881..53798 + 918 WP_088212666 type-F conjugative transfer system secretin TraK traK
RCR42_RS25875 (RCR42_25875) 53798..55111 + 1314 WP_308982260 TrbI/VirB10 family protein traB
RCR42_RS25880 (RCR42_25880) 55108..55686 + 579 WP_088212668 type IV conjugative transfer system lipoprotein TraV traV
RCR42_RS25885 (RCR42_25885) 55690..56082 + 393 WP_000479535 TraA family conjugative transfer protein -
RCR42_RS25890 (RCR42_25890) 56283..61769 + 5487 WP_308982212 Ig-like domain-containing protein -
RCR42_RS25895 (RCR42_25895) 61918..62625 + 708 WP_001259346 DsbC family protein -
RCR42_RS25900 (RCR42_25900) 62622..65069 + 2448 WP_088212670 type IV secretion system protein TraC virb4
RCR42_RS25905 (RCR42_25905) 65084..65401 + 318 WP_000351984 hypothetical protein -
RCR42_RS25910 (RCR42_25910) 65398..65928 + 531 WP_001010738 S26 family signal peptidase -
RCR42_RS25915 (RCR42_25915) 65891..67156 + 1266 WP_088212671 TrbC family F-type conjugative pilus assembly protein traW
RCR42_RS25920 (RCR42_25920) 67153..67824 + 672 WP_088212672 EAL domain-containing protein -
RCR42_RS25925 (RCR42_25925) 67824..68828 + 1005 WP_005507569 TraU family protein traU
RCR42_RS25930 (RCR42_25930) 68944..71742 + 2799 WP_204260578 conjugal transfer mating pair stabilization protein TraN traN
RCR42_RS25935 (RCR42_25935) 71782..72642 - 861 WP_088212674 hypothetical protein -
RCR42_RS25940 (RCR42_25940) 72765..73406 - 642 WP_088212675 hypothetical protein -
RCR42_RS25945 (RCR42_25945) 73702..74022 + 321 WP_163440224 hypothetical protein -
RCR42_RS25950 (RCR42_25950) 74326..74511 + 186 WP_001186916 hypothetical protein -
RCR42_RS25955 (RCR42_25955) 74731..75699 + 969 WP_113620223 AAA family ATPase -
RCR42_RS25960 (RCR42_25960) 75710..76615 + 906 WP_308982217 hypothetical protein -

Host bacterium

ID   15655 GenBank   NZ_CP133339
Plasmid name   pC-CRECL423 Incompatibility group   IncA/C2
Plasmid size   147899 bp Coordinate of oriT [Strand]   48512..48662 [+]
Host baterium   Enterobacter hormaechei strain CRECL423

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -