Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115210 |
| Name | oriT_pEh16_OXA48  |
| Organism | Enterobacter hormaechei strain Ehh_16 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP127106 (39818..39923 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pEh16_OXA48
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 9721 | GenBank | WP_004187323 |
| Name | traI_QPR55_RS24200_pEh16_OXA48 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 11337 | GenBank | WP_004206885 |
| Name | t4cp2_QPR55_RS23905_pEh16_OXA48 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 42719..62008
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QPR55_RS24180 (QPR55_24185) | 37803..38114 | + | 312 | WP_004187333 | hypothetical protein | - |
| QPR55_RS24185 (QPR55_24190) | 38247..38783 | + | 537 | WP_004187332 | hypothetical protein | - |
| QPR55_RS24190 (QPR55_24195) | 39285..39680 | - | 396 | WP_019725163 | hypothetical protein | - |
| QPR55_RS24195 (QPR55_24200) | 39715..40242 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| QPR55_RS24200 (QPR55_24205) | 40229..42208 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| QPR55_RS24205 (QPR55_24210) | 42222..42722 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| QPR55_RS24210 (QPR55_24215) | 42719..43498 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| QPR55_RS24215 (QPR55_24220) | 43509..44672 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| QPR55_RS24220 (QPR55_24225) | 44662..44922 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| QPR55_RS24225 (QPR55_24230) | 44947..48384 | + | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
| QPR55_RS24230 (QPR55_24235) | 48350..48862 | + | 513 | WP_011091071 | hypothetical protein | traL |
| QPR55_RS24235 (QPR55_24240) | 48863..49075 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| QPR55_RS24240 (QPR55_24245) | 49047..49457 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| QPR55_RS24245 (QPR55_24250) | 49525..50238 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| QPR55_RS24250 (QPR55_24255) | 50247..51398 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| QPR55_RS24255 (QPR55_24260) | 51410..52759 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| QPR55_RS24260 (QPR55_24265) | 52771..53475 | + | 705 | WP_031942491 | conjugal transfer protein TraP | traP |
| QPR55_RS24265 (QPR55_24270) | 53499..54029 | + | 531 | WP_104011266 | conjugal transfer protein TraQ | traQ |
| QPR55_RS24270 (QPR55_24275) | 54046..54435 | + | 390 | WP_011091078 | DUF6750 family protein | traR |
| QPR55_RS24275 (QPR55_24280) | 54481..54975 | + | 495 | WP_011091080 | hypothetical protein | - |
| QPR55_RS24280 (QPR55_24285) | 54972..58022 | + | 3051 | WP_031942490 | plasmid transfer protein | traU |
| QPR55_RS24285 (QPR55_24290) | 58052..59227 | + | 1176 | WP_167577398 | conjugal transfer protein TraW | traW |
| QPR55_RS24290 (QPR55_24295) | 59224..59820 | + | 597 | WP_015060003 | hypothetical protein | - |
| QPR55_RS24295 (QPR55_24300) | 59813..62008 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| QPR55_RS24300 (QPR55_24305) | 62010..62663 | + | 654 | WP_015060005 | hypothetical protein | - |
| QPR55_RS24305 (QPR55_24310) | 62744..62974 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 15645 | GenBank | NZ_CP127106 |
| Plasmid name | pEh16_OXA48 | Incompatibility group | IncL/M |
| Plasmid size | 63269 bp | Coordinate of oriT [Strand] | 39818..39923 [+] |
| Host baterium | Enterobacter hormaechei strain Ehh_16 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |