Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115151
Name   oriT_NBSH29|unnamed3 in_silico
Organism   Mesorhizobium sp. NBSH29
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP045495 (46687..46723 [+], 37 nt)
oriT length   37 nt
IRs (inverted repeats)      22..27, 32..37  (CGTCGC..GCGACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 37 nt

>oriT_NBSH29|unnamed3
GGATCCAAGGGCGCAGTAATACGTCGCTGACGCGACG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9686 GenBank   WP_195165248
Name   traA_GA830_RS19380_NBSH29|unnamed3 insolico UniProt ID   _
Length   1100 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1100 a.a.        Molecular weight: 124141.91 Da        Isoelectric Point: 10.0770

>WP_195165248.1 Ti-type conjugative transfer relaxase TraA [Mesorhizobium sp. NBSH29]
MAITHFTPQLIGRGNGRSAVLSAAYRHCAKMIYEMEARTVDYSNKRNLAHEEFLAPPDAPDWVCKLLADR
SVSGAAEAFWNRVEAFEKREDAQFAKEFIIALPVELTKEQNIALMRQFVFEQVLSRGQVADWVYHDDPGN
PHVHLMTTLRPLTETGFGTKKIAVLGEDGQPLRTKTGKIQYRLWAGEKKEFLEQRNGWLDLQNQHLALGG
IDLRVDGRSYAERGIELVATAHIGVGAKAIQRKGERQGWTPALDRIELFEAQREENARRILRRPEVVLDK
ISSEKSVFDERDIAKILHRYVDDAGTFRQLMARILQSPDLLRIERESVDFGTGERVRARYTTRELIRVEA
EMAKQSIWLSQRTSHGVRETVLRATFERHAQLSREQRVAIEHVADAAQIAAVVGRAGAGKTTMMKAAREA
WEMAGYRVVGAALAGKAAEGLEKEAGIASRTLASWELRWKDGRDALDAKTVFVLDEAGMVASKQMALFVE
AALRTGAKLVLVGDPEQLQPIEAGAAFRAIVERTGYAELETIYRQREEWMRVASLDLARGRVDQAVSAYR
KQGRVLGSELKAEAVANLIDDWNRDYDAVKTTLILAHLRRDVRMLNVMAREKLVERGLVREGHVFHTEDG
VRRFDTGDQIVFLKNDSALGVKNGMIGRVVEAAADRIVAEVGEGDQRHRVSVDQRFYRNVDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDMALYYGRRSFGFHGGLIKILSKRNAKETTLDYERGTLY
RQALRFAENRGLHVVRVARTLVRDRVDWTLRQKQRLADLALRLRTLSERLGLLDTRPTQTLKEAAPMVKG
VTLFPKSVQDTVEEKLLADPVVKKQWEEVSTRFRFVFADPETAFRAMKFDAVLTDPSTARTTLERLVNEP
ATIGPLKGKDGLLARKSDREDRRIAEVNAPALKRDIERYLRLREGAVQKIATDEQVLRRRVSIDIPALSP
AAHCVLERVRDAIDRNDLPAALGYALADRMAKAEIDGFNKAITERLGERTLLTNAAREPSGKLFDRAAEG
LAPGERQKLAEAWPNMRAAQQLAAHERTVETLKRTENLRLSQRQSPVLKQ

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(17-243)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(855-951)

AAA domain (AAA_30)

(381-565)


  Protein structure



No available structure.




T4CP

ID   11296 GenBank   WP_195165245
Name   traG_GA830_RS19365_NBSH29|unnamed3 insolico UniProt ID   _
Length   641 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 641 a.a.        Molecular weight: 68863.75 Da        Isoelectric Point: 8.5507

>WP_195165245.1 Ti-type conjugative transfer system protein TraG [Mesorhizobium sp. NBSH29]
MTANRILLAGSAATLMLGACIGISGIETWLVTFGTSDAARLMLGRTGIALPYIAAAVIGVIFLFASAGAN
AIRAAGWGVACGAASVIAIAVAREAVRLSALADCVPAGKAILSYVDPATMVGATVAMICGGFALRVTMHG
NAAFALAAPRRIRGKRAVHGEADWMSMPAAARLFPDSGGIVIGERYRVDRDSVAAQSFRADARETWGAGG
RSPLLCFDGSFGSSHGVVFAGSGGFKTTSVTVPTALKWGGSLVVLDPSSEVAPMVIDHRKRAGRNVFLLD
PGNAAIGFNALDWIGRFGSTKEEDIVAVATWIITDNPRQASARDDFFRASALQLLTVLIADVCLSGHTEE
KDQTLRRVRSNLSEPEPKLRERLTAIHDQSESAFVKENVAPFIAMTPETFSGVYANAVKETHWLSYSNYA
ALVSGSSFTTDELAAGETDVFIALDLKVLETHPGLARVIIGALMNAIYNRNGEVKGRTLFLLDEVARLGF
LRVLETARDAGRKYGITMTLLYQSIGQMREAYGGRDATSKWFESASWVSFAAINDPETADYISKRCGDTT
VEVDQTSRSSQMSGSSRTRSKQLTRRPLILSHEVMQMRADEQIVFTAGNPPLRCGRAIWFRRADMKACVG
ENRFHRKETEA

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(164-639)

  Protein structure



No available structure.



ID   11297 GenBank   WP_195165259
Name   t4cp2_GA830_RS19450_NBSH29|unnamed3 insolico UniProt ID   _
Length   818 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 818 a.a.        Molecular weight: 91501.91 Da        Isoelectric Point: 5.4835

>WP_195165259.1 conjugal transfer protein TrbE [Mesorhizobium sp. NBSH29]
MAALRSFRHSGPSFSDLVPYAALVANGVILLKDGSLMAGWYFAGPDSESSTDAERNEVSRQINTVLSRLG
SGWMIQVEAVRVETSDYPAREQCHFPDPVTLAIDGERQAHFQKERGHYESRHALILTWRPPEKTRSGLAR
YVYSDSESRAARYADTVLDSFQTSIREVEQYLGNLLSIRRMVTQSVEERGGFRIARYDELFQFIRFCITG
ENHPIRLPEIPMYLDWLATAEFDHGLSPMVENHYLAVVAIDGFPAESWPGILNSLDLMPLTYRWSSRFIF
LDDQEARQKLERTRKKWQQKVRPFFDQLFQTQSRSVDQDAMMMVAETEDAIAQASSQLVAYGYYTPVIVL
FDDDEARLREKAEAVRRLIQAEGFGARIETVNATEAFLGSLPGNWYANIREPLINTRNLADLIPLNSVWS
GAAAAPCPFYPPASPPLMLVASGSTPFRLNLHVDDVGHTLIFGPTGSGKSTLLSLIAAQFRRYTFAQIFA
FDKGRSMLPLTLAAGGDHYEIGETEGEGGPRLAFCPLSDLATDGDRAWASEWIETLVVLQGVPITPDHRN
AISRQVALMADSSGRSLSDFVSGVQLREVKEALHHYTVDGPLGQLLDAERDGLSLGSFQTFEIEQLMNMG
ERSLVPVLLYLFRRIETRLTGAPSLIILDEAWLMLGHATFRDKIREWLKVLRKANCAVVLATQSISDAER
SGIIDVLKESCPTKICLPNGAARERGTREFYERIGFNERQIEIVATALPKREYYVVSPDGRRLFDMALGP
VALAFAGASGKEDLKRIQALSAEHGGDWPVHWLQQRGIGDAETILQRG

  Protein domains


Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(193-400)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 44122..65425

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GA830_RS19330 (GA830_19430) 39351..39935 + 585 WP_195165240 hypothetical protein -
GA830_RS19335 (GA830_19435) 40288..41214 + 927 WP_195165241 ArdC family protein -
GA830_RS19340 (GA830_19440) 41214..41402 + 189 WP_195165291 hypothetical protein -
GA830_RS19345 (GA830_19450) 42012..42326 + 315 WP_195165242 DUF736 domain-containing protein -
GA830_RS19350 (GA830_19455) 42407..42598 + 192 WP_195165243 hypothetical protein -
GA830_RS19355 (GA830_19460) 42918..43163 - 246 WP_195165244 WGR domain-containing protein -
GA830_RS19360 (GA830_19465) 43248..43605 - 358 Protein_52 thermonuclease family protein -
GA830_RS19365 (GA830_19470) 44122..46047 - 1926 WP_195165245 Ti-type conjugative transfer system protein TraG virb4
GA830_RS19370 (GA830_19475) 46034..46249 - 216 WP_195165246 type IV conjugative transfer system coupling protein TraD -
GA830_RS19375 (GA830_19480) 46254..46550 - 297 WP_195165247 conjugal transfer protein TraC -
GA830_RS19380 (GA830_19485) 46803..50105 + 3303 WP_195165248 Ti-type conjugative transfer relaxase TraA -
GA830_RS19385 (GA830_19490) 50102..50632 + 531 WP_195165249 conjugative transfer signal peptidase TraF -
GA830_RS19390 (GA830_19495) 50553..51788 + 1236 WP_195165250 conjugal transfer protein TraB -
GA830_RS19395 (GA830_19500) 51803..52435 + 633 WP_195165251 TraH family protein virB1
GA830_RS19400 (GA830_19505) 52743..53069 + 327 WP_195165252 transcriptional repressor TraM -
GA830_RS20230 53088..53414 - 327 WP_258045743 LuxR C-terminal-related transcriptional regulator -
GA830_RS20235 53392..53721 - 330 WP_258045744 autoinducer binding domain-containing protein -
GA830_RS19410 (GA830_19515) 54348..55415 + 1068 WP_195165253 HPP family protein -
GA830_RS19415 (GA830_19520) 55856..57163 - 1308 WP_195165254 IncP-type conjugal transfer protein TrbI virB10
GA830_RS19420 (GA830_19525) 57176..57628 - 453 WP_195165255 conjugal transfer protein TrbH -
GA830_RS19425 (GA830_19530) 57632..58471 - 840 WP_195165287 P-type conjugative transfer protein TrbG virB9
GA830_RS19430 (GA830_19535) 58489..59151 - 663 WP_195165256 conjugal transfer protein TrbF virB8
GA830_RS19435 (GA830_19540) 59169..60356 - 1188 WP_195165257 P-type conjugative transfer protein TrbL virB6
GA830_RS19440 (GA830_19545) 60350..60559 - 210 WP_258045720 entry exclusion protein TrbK -
GA830_RS19445 (GA830_19550) 60556..61296 - 741 WP_258045749 P-type conjugative transfer protein TrbJ virB5
GA830_RS19450 (GA830_19555) 61328..63784 - 2457 WP_195165259 conjugal transfer protein TrbE virb4
GA830_RS19455 (GA830_19560) 63795..64106 - 312 WP_258045721 conjugal transfer protein TrbD virB3
GA830_RS19460 (GA830_19565) 64099..64467 - 369 WP_195165134 TrbC/VirB2 family protein virB2
GA830_RS19465 (GA830_19570) 64457..65425 - 969 WP_195165135 P-type conjugative transfer ATPase TrbB virB11
GA830_RS19470 65444..65593 - 150 WP_195165136 hypothetical protein -
GA830_RS19475 (GA830_19575) 65989..67167 + 1179 WP_195165288 plasmid partitioning protein RepA -
GA830_RS19480 (GA830_19580) 67243..68241 + 999 WP_195165137 plasmid partitioning protein RepB -
GA830_RS19485 (GA830_19585) 68412..69755 + 1344 WP_195165260 plasmid replication protein RepC -

Host bacterium

ID   15586 GenBank   NZ_CP045495
Plasmid name   NBSH29|unnamed3 Incompatibility group   -
Plasmid size   140849 bp Coordinate of oriT [Strand]   46687..46723 [+]
Host baterium   Mesorhizobium sp. NBSH29

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   mreA, ncrA, dmeF
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -