Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115150
Name   oriT_NBSH29|unnamed2 in_silico
Organism   Mesorhizobium sp. NBSH29
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP045494 (56194..56230 [+], 37 nt)
oriT length   37 nt
IRs (inverted repeats)      22..27, 32..37  (CGTCGC..GCGACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 37 nt

>oriT_NBSH29|unnamed2
GGTTCCAAGGGCGCAGTAATACGTCGCTGAGGCGACG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9685 GenBank   WP_195165167
Name   traA_GA830_RS18810_NBSH29|unnamed2 insolico UniProt ID   _
Length   1100 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1100 a.a.        Molecular weight: 123946.76 Da        Isoelectric Point: 10.2479

>WP_195165167.1 Ti-type conjugative transfer relaxase TraA [Mesorhizobium sp. NBSH29]
MAITHFTPQLIGRGNGRSAVLSAAYRHCAKMTYEMEARTVDYSNKRNLAHEEFLLPPDSPKWATALIADR
SVSGAAEAFWNRIEAFEKREDAQFAKEFIIALPVELTKEQNVALMRQFVFEQVLARGQVADWVYHDEPGN
PHVHLMTTLRPLIEKGFGAKKIAVLGEDGQAVRTKTGKIQYRLWAGEKKEFLEQRNGWLDLQNQHLAFGG
FEIRVDGRSYAERGIDLVATVHIGVGAKAIQRKGERQGWTPALDRIELFEAQREENARRILRRPEIVLDK
ISSEKSVFDERDIAKVLHRYVDDAGTFQQLMARILQSPDLLRIERESVDFGTGERVRARYTTRELIRVEA
EMAKQTIWLSQRTSHGVRETVLQATFERHAQLSREQRVAIEHVVDEARIAAVVGRAGAGKTTMMKAAREA
WEMAGYRVVGGALAGKAAEGLEKEAGIASRTLASWELRWKQGRDALDAKTVFVLDEAGMVASKQMALFVE
AALRTGAKLVLVGDPEQLQPIAAGAAFRAIVERTGYAELETIYRQREEWMRVASLDLARGRVDQAVSAYR
KQGKVLGSELKAEAVVNLIDDWNRDYDAAKTTLILAHLRRDVRMLNVMAREKLVERGIVGEGHAFRTEDG
MRRFDAGDQIVFLKNDSALGVKNGMIGRVVEAAANRIVAEVGEGDQRHRVSVDQRFYRNVDHGYATTIHK
SQGSTVDRVKVLASLSLDRHLTYVAMTRHREDMALYYGRRSFGFNGGLIKILSKRNAKETTLDYERGTLY
RQGLRFAENRGLHIARVARTLVRDRVDWTLRQKQRLADLALRLRTLSERIGLVDTRQTQTLKEAAPMVKG
VTLFPKSAQDTVEEKLLADPAVKKQWEEVSTRFRFVFANPETAFRAMKFDAVLADPSTARTTLERLVNEP
ATIGPLKGRVGLLAGKSDREDRRIAEVNAPALKRDIERYLRLREGAVQKIEVDEQTLRRRVSIDIPALSP
AAHLVLERVRDAIDRNDLPAALGYALADRMAKAEIDGFNKAITERFGERTLITNAAREPSGKLFDRAAEG
LAPGQRQKLAEAWPVMRAAQRIAAHERTAETLKQTENLRLSQRQIPVLKQ

  Protein domains


Predicted by InterproScan.

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(855-951)

MobA/MobL family (MobA_MobL)

(17-243)

AAA domain (AAA_30)

(381-565)


  Protein structure



No available structure.




T4CP

ID   11294 GenBank   WP_195165164
Name   traG_GA830_RS18795_NBSH29|unnamed2 insolico UniProt ID   _
Length   641 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 641 a.a.        Molecular weight: 69519.45 Da        Isoelectric Point: 9.3565

>WP_195165164.1 Ti-type conjugative transfer system protein TraG [Mesorhizobium sp. NBSH29]
MTANRILLAGAAVTLMLAACIGISGIETWLVTFGTSDTARLMLRRIGIVLPYAIAAGTGIVFLFAAKGAL
AIRSAGAGVLIGAMAVTLIAGTREARRLAAFYDALPVGDTILEYADPAIGMGAILACIVGAFAGRVAIRG
NAAFAASTPRRVRGKRAIHGEANWMTMNDAKRLFPKTGGILVGEAYRVDKGAVADRGFRADNRETWGRGG
KSPLLCFDASFGSTHGLVFAGSGGFKTTSVTVPTALKWGGGLVALDPSNEVAPMVIEHRRKAGRDVFVLD
PKNPATGFNVLDWIGRFGGTREEDVVAVASWVVTDTGRSTSVRDDFFRATALQLITALIADVCLSGHTEK
ENQHLRQMRANLSEPEPKLRERLQKIYDNSESEFVKENVAPFIAMTPETFSGVYANAVKETHWLSYPNYA
ALVSGSSFATDDIADGKTDIFVNLDLKTLETHPGLARTIIGALLNAIYNRNGEVTGRTLFLLDEVARLGY
LRILETARDAGRKYGISLVLLFQSIGQMRETYGGRDASSKWFESASWISFAAINDPETAEYISRRCGNTT
VEVDQLSRSSQMSGSSRTRSRQLASRPLILPDEVLRMRGDEQIVFTAGNAPLRCGRAIWFRRDDMKSVTG
ENRFHRKEEEA

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(163-637)

  Protein structure



No available structure.



ID   11295 GenBank   WP_195165181
Name   t4cp2_GA830_RS18895_NBSH29|unnamed2 insolico UniProt ID   _
Length   818 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 818 a.a.        Molecular weight: 91642.22 Da        Isoelectric Point: 5.7509

>WP_195165181.1 conjugal transfer protein TrbE [Mesorhizobium sp. NBSH29]
MVALSSFRHSGPSFSDLVPYAALVANGVILLKDGSLMAGWYFAGPDSDSSTDPERNEVSRQINLILARLG
SGWMIQVEAVRVATSDYPAREQCHFPDPVTRAIDDERRAHFQEGRGHFESTHALILTWRPPEKTRSGLAR
YVYSDTESRVARYADTVLDSFQTSIREVEQYLGNLLSIRRMVTQLVEERGGFQTARYDELFQFIRFCITG
ENHPIRLPEIPMYLDWLATAEFHHGLSPMVENHYLAVVAIDGFPAESWPGILNSLDLMPMTYRWSSRFIV
LDEQEARQKLERTRKKWQQKVRPFFDQLFQTQSRSVDQDAMMMVAETEDAIAQASSQLVAYGYYTPAIVL
FDDNETRLREKAEAVRRLIQAEGFGARIETMNATEAFLGSLPGNWYANIREPLINTRNLADLIPLNSVWS
GAPTAPCPFYPPASPPLMLVASGSTPFRLNLHVDDVGHTLIFGPTGSGKSTLLALIAAQFRRYAHSQIFA
FDKGRSMLPLTLAAGGDHYEIGESEGEGGPRLAFCPLSDLASDGDRAWASEWIETLVVLQGVTITPDHRN
AISRQVALMADSSGRSLSDFVSGVQLREIKEALHHYTVDGPLGQLLDAESDGLSLRSFQTFEIEQLMNMG
ERSLVPVLLYLFRRIEKRLTGEPSLIILDEAWLMLGHPTFRDKIREWLKVLRKANCAVVLATQSISDAER
SGIIDVLKESCPTKICLPNGAARERGTREFYERIGFNERQIEIVATALPKREYYVVSADGRRLFDMALGP
IALSFAGASGKDDLKRIVALKAEQGGDWPIHWLQQRGIGDAETILHRG

  Protein domains


Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(188-400)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 53629..76688

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GA830_RS18755 (GA830_18830) 48820..50649 + 1830 WP_258045732 integrase -
GA830_RS18760 (GA830_18835) 50618..51100 + 483 WP_195165158 hypothetical protein -
GA830_RS18765 (GA830_18840) 51582..51878 + 297 WP_195165159 DUF736 domain-containing protein -
GA830_RS18770 (GA830_18845) 52007..52294 + 288 Protein_58 DUF736 domain-containing protein -
GA830_RS18775 (GA830_18850) 52411..52680 + 270 WP_195165160 type II toxin-antitoxin system prevent-host-death family antitoxin -
GA830_RS18780 (GA830_18855) 52677..53090 + 414 WP_195165161 hypothetical protein -
GA830_RS18785 (GA830_18860) 53108..53359 - 252 WP_195165162 WGR domain-containing protein -
GA830_RS18790 (GA830_18865) 53402..53632 - 231 WP_195165163 hypothetical protein -
GA830_RS18795 (GA830_18870) 53629..55554 - 1926 WP_195165164 Ti-type conjugative transfer system protein TraG virb4
GA830_RS18800 (GA830_18875) 55541..55756 - 216 WP_195165165 type IV conjugative transfer system coupling protein TraD -
GA830_RS18805 (GA830_18880) 55761..56057 - 297 WP_195165166 conjugal transfer protein TraC -
GA830_RS18810 (GA830_18885) 56309..59611 + 3303 WP_195165167 Ti-type conjugative transfer relaxase TraA -
GA830_RS18815 (GA830_18890) 59608..60138 + 531 WP_195165168 conjugative transfer signal peptidase TraF -
GA830_RS18820 (GA830_18895) 60155..61294 + 1140 WP_258045734 conjugal transfer protein TraB -
GA830_RS18825 (GA830_18900) 61309..61923 + 615 WP_195165170 TraH family protein virB1
GA830_RS18830 (GA830_18905) 62226..62546 + 321 WP_195165171 transcriptional repressor TraM -
GA830_RS18835 (GA830_18910) 62561..63265 - 705 WP_258045735 autoinducer binding domain-containing protein -
GA830_RS18840 (GA830_18915) 63328..63690 - 363 WP_195165172 hypothetical protein -
GA830_RS18845 (GA830_18920) 63687..64322 - 636 Protein_73 transposase -
GA830_RS18850 (GA830_18925) 64438..66075 + 1638 WP_195165173 RcgA family putative transporter -
GA830_RS18855 (GA830_18930) 66065..67090 + 1026 WP_195165174 S9 family peptidase -
GA830_RS18860 (GA830_18935) 67123..68424 - 1302 WP_195165175 IncP-type conjugal transfer protein TrbI virB10
GA830_RS18865 (GA830_18940) 68436..68870 - 435 WP_195165176 conjugal transfer protein TrbH -
GA830_RS18870 (GA830_18945) 68873..69712 - 840 WP_195165206 P-type conjugative transfer protein TrbG virB9
GA830_RS18875 (GA830_18950) 69730..70392 - 663 WP_195165177 conjugal transfer protein TrbF virB8
GA830_RS18880 (GA830_18955) 70411..71601 - 1191 WP_195165178 P-type conjugative transfer protein TrbL virB6
GA830_RS18885 (GA830_18960) 71595..71813 - 219 WP_195165179 entry exclusion protein TrbK -
GA830_RS18890 (GA830_18965) 71810..72550 - 741 WP_258045739 P-type conjugative transfer protein TrbJ virB5
GA830_RS18895 (GA830_18970) 72582..75038 - 2457 WP_195165181 conjugal transfer protein TrbE virb4
GA830_RS18900 (GA830_18975) 75049..75363 - 315 WP_195165182 conjugal transfer protein TrbD virB3
GA830_RS18905 (GA830_18980) 75356..75730 - 375 WP_195165183 TrbC/VirB2 family protein virB2
GA830_RS18910 (GA830_18985) 75720..76688 - 969 WP_195165184 P-type conjugative transfer ATPase TrbB virB11
GA830_RS18915 (GA830_18990) 77247..78455 + 1209 WP_195165185 plasmid partitioning protein RepA -
GA830_RS18920 (GA830_18995) 78646..79647 + 1002 WP_195165186 plasmid partitioning protein RepB -
GA830_RS18925 (GA830_19000) 79829..81148 + 1320 WP_195165187 plasmid replication protein RepC -

Host bacterium

ID   15585 GenBank   NZ_CP045494
Plasmid name   NBSH29|unnamed2 Incompatibility group   -
Plasmid size   118320 bp Coordinate of oriT [Strand]   56194..56230 [+]
Host baterium   Mesorhizobium sp. NBSH29

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -