Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115060 |
| Name | oriT_NZ_OW849493  |
| Organism | Serratia marcescens isolate 0 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OW849493 (55940..56045 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_NZ_OW849493
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 9638 | GenBank | WP_004187323 |
| Name | traI_LQ146_RS27865_NZ_OW849493 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 11245 | GenBank | WP_004206885 |
| Name | t4cp2_LQ146_RS27475_NZ_OW849493 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 58841..78130
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LQ146_RS27845 (AI2617V1_5322) | 53925..54236 | + | 312 | WP_004187333 | hypothetical protein | - |
| LQ146_RS27850 (AI2617V1_5323) | 54369..54905 | + | 537 | WP_004187332 | hypothetical protein | - |
| LQ146_RS27855 (AI2617V1_5324) | 55407..55802 | - | 396 | WP_019725163 | hypothetical protein | - |
| LQ146_RS27860 (AI2617V1_5325) | 55837..56364 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| LQ146_RS27865 (AI2617V1_5326) | 56351..58330 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| LQ146_RS27870 (AI2617V1_5327) | 58344..58844 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| LQ146_RS27875 (AI2617V1_5328) | 58841..59620 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| LQ146_RS27880 (AI2617V1_5329) | 59631..60794 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| LQ146_RS27885 (AI2617V1_5330) | 60784..61044 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| LQ146_RS27890 (AI2617V1_5331) | 61069..64506 | + | 3438 | WP_225373171 | LPD7 domain-containing protein | - |
| LQ146_RS27895 (AI2617V1_5332) | 64472..64984 | + | 513 | WP_011091071 | hypothetical protein | traL |
| LQ146_RS27900 | 64985..65197 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| LQ146_RS27905 | 65169..65579 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| LQ146_RS27910 (AI2617V1_5334) | 65647..66360 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| LQ146_RS27915 (AI2617V1_5335) | 66369..67520 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| LQ146_RS27920 (AI2617V1_5336) | 67532..68881 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| LQ146_RS27925 (AI2617V1_5337) | 68893..69597 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| LQ146_RS27930 (AI2617V1_5338) | 69621..70151 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| LQ146_RS27935 (AI2617V1_5339) | 70168..70557 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| LQ146_RS27940 (AI2617V1_5340) | 70603..71097 | + | 495 | WP_004187480 | hypothetical protein | - |
| LQ146_RS27945 (AI2617V1_5341) | 71094..74144 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| LQ146_RS27950 (AI2617V1_5342) | 74141..75349 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| LQ146_RS27955 (AI2617V1_5343) | 75346..75942 | + | 597 | WP_015060003 | hypothetical protein | - |
| LQ146_RS27960 (AI2617V1_5344) | 75935..78130 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| LQ146_RS27965 (AI2617V1_5345) | 78132..78785 | + | 654 | WP_015060005 | hypothetical protein | - |
| LQ146_RS27970 (AI2617V1_5346) | 78866..79096 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 15495 | GenBank | NZ_OW849493 |
| Plasmid name | NZ_OW849493 | Incompatibility group | IncL/M |
| Plasmid size | 79391 bp | Coordinate of oriT [Strand] | 55940..56045 [+] |
| Host baterium | Serratia marcescens isolate 0 |
Cargo genes
| Drug resistance gene | catA1, blaVIM-1, dfrB1, ant(3'')-Ia, catB2, qacE, sul1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |