Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115059
Name   oriT_NZ_OW849492 in_silico
Organism   Serratia marcescens isolate 0
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OW849492 (143555..143705 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      12..17, 24..29  (AACCCG..CGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_NZ_OW849492
TGGTCGATGTGAACCCGTTTGACCGGGTTATGAATGAGTTGAAAAGTCGTGGCCGCAAGAACGCTCATATCCTGAGCATCCTTCAATTCGACTGGCCCGCATCGGAGGTCATCATCGAGAAGCTGAGCTGTTACATCACAGATGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9637 GenBank   WP_000909947
Name   mobH_LQ146_RS27185_NZ_OW849492 insolico UniProt ID   _
Length   987 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 987 a.a.        Molecular weight: 109404.96 Da        Isoelectric Point: 4.8922

>WP_000909947.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALHKLFGGRSGAIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWATQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAYRHGIDRYFIRWRDKRHKRHEQFSL
LAWHRIIPAETQEFLSKSGPSIIEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVNEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISQDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGPVKILMLRLESNDLVFTTEPPAAVTGEVVGDVEDAEIE
FVDPEEADEDQAEGDAGLNNDMLAAELEAEKALAGLGFGDAMAMLKSTSDIAEEKPEQEDTESTELPKPD
ASKKGKQQSKPGKARAEKQPQKPEVKEDLSPQDIAKNAPPLANDDPLQALKDVGGGLGDIDFPFDVFNAS
AETACTDATNLEIPDITEPEKQEEQPKPDFVPQEQNSLQNGDFPIFSDSDEPPSWAIEPLPMLTDTPEQT
IPAPDMQHAGKPKELEKDAKTLLSEMLAGYGEASTLLEQAIMPVLEGKTTLGEVLCLMKGQAVILYPEGA
RSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLVDQLSDAVVAAIKDAEASMGGYQEAFELV
SPPGSGVSKNKSAPKQQSRKKTQQQKPEVDAGAGSEQKTKDKSLQQQPKEKQGNVASLVEEQKRKPVQDQ
EKQNVARLPKREAQPVAPKPKVEHERELGHVEVRERDEPEVREFEPPKAKTNPKDINEEDFLPSGVTPEK
ALLMLKDMIQKRSGRWLVTPVLEEDGCLVTSVKAFDMIAGENVGISKHILYGLLSRAQRRPLLKKRQGKL
YLEVDKA

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure



No available structure.




T4CP

ID   11243 GenBank   WP_000178858
Name   traD_LQ146_RS27190_NZ_OW849492 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69248.76 Da        Isoelectric Point: 7.1093

>WP_000178858.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWMAASCAALAVEQVSTMPSEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPEDMWLGSGFLWENRHAQRVFEILKRDWTSIVGKESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVLTHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVKPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNTDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   11244 GenBank   WP_051479468
Name   traC_LQ146_RS27265_NZ_OW849492 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92523.78 Da        Isoelectric Point: 5.9204

>WP_051479468.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIITIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDQSVGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTSLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRIFAKTSKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQHLAQIRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYDDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSQKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSTFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSDGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRKQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 141639..166743

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ146_RS27175 137679..137912 - 234 WP_039272424 hypothetical protein -
LQ146_RS27180 (AI2617V1_5195) 137894..138511 - 618 WP_012766323 hypothetical protein -
LQ146_RS27185 (AI2617V1_5196) 138679..141642 + 2964 WP_000909947 MobH family relaxase -
LQ146_RS27190 (AI2617V1_5197) 141639..143504 + 1866 WP_000178858 conjugative transfer system coupling protein TraD virb4
LQ146_RS27195 (AI2617V1_5198) 143554..144099 + 546 WP_023356350 hypothetical protein -
LQ146_RS27200 (AI2617V1_5199) 144056..144685 + 630 WP_000743448 DUF4400 domain-containing protein tfc7
LQ146_RS27205 (AI2617V1_5200) 144696..145142 + 447 WP_039272421 hypothetical protein -
LQ146_RS27210 (AI2617V1_5201) 145152..145529 + 378 WP_000869260 hypothetical protein -
LQ146_RS27215 (AI2617V1_5202) 145529..146191 + 663 WP_039272419 hypothetical protein -
LQ146_RS27220 146526..146873 + 348 WP_039272417 hypothetical protein -
LQ146_RS27225 (AI2617V1_5204) 147016..147297 + 282 WP_000805626 type IV conjugative transfer system protein TraL traL
LQ146_RS27230 (AI2617V1_5205) 147294..147920 + 627 WP_039272415 TraE/TraK family type IV conjugative transfer system protein traE
LQ146_RS27235 (AI2617V1_5206) 147904..148821 + 918 WP_012766326 type-F conjugative transfer system secretin TraK traK
LQ146_RS27240 (AI2617V1_5207) 148818..150134 + 1317 WP_039272412 TraB/VirB10 family protein traB
LQ146_RS27245 (AI2617V1_5208) 150131..150709 + 579 WP_039272410 type IV conjugative transfer system lipoprotein TraV traV
LQ146_RS27250 (AI2617V1_5209) 150713..151105 + 393 WP_012766327 TraA family conjugative transfer protein -
LQ146_RS27255 (AI2617V1_5210) 151306..156792 + 5487 WP_039272407 Ig-like domain-containing protein -
LQ146_RS27260 (AI2617V1_5211) 156941..157648 + 708 WP_001259344 DsbC family protein -
LQ146_RS27265 (AI2617V1_5212) 157645..160092 + 2448 WP_051479468 type IV secretion system protein TraC virb4
LQ146_RS27270 (AI2617V1_5213) 160107..160424 + 318 WP_000364398 hypothetical protein -
LQ146_RS27275 (AI2617V1_5214) 160421..160951 + 531 WP_000672456 S26 family signal peptidase -
LQ146_RS27280 (AI2617V1_5215) 160914..162182 + 1269 WP_039272404 TrbC family F-type conjugative pilus assembly protein traW
LQ146_RS27285 (AI2617V1_5216) 162179..162856 + 678 WP_039272402 EAL domain-containing protein -
LQ146_RS27290 (AI2617V1_5217) 162850..163854 + 1005 WP_000911304 TraU family protein traU
LQ146_RS27295 (AI2617V1_5218) 164005..166743 + 2739 WP_039272401 conjugal transfer mating pair stabilization protein TraN traN
LQ146_RS27300 (AI2617V1_5219) 166782..167642 - 861 WP_230122741 hypothetical protein -
LQ146_RS27305 (AI2617V1_5220) 167754..168404 - 651 WP_000796641 hypothetical protein -
LQ146_RS27310 (AI2617V1_5221) 168696..169016 + 321 WP_012766334 hypothetical protein -
LQ146_RS27315 (AI2617V1_5222) 169327..169512 + 186 WP_001186892 hypothetical protein -
LQ146_RS27320 (AI2617V1_5223) 169733..170701 + 969 WP_001553257 AAA family ATPase -
LQ146_RS27325 (AI2617V1_5224) 170713..171618 + 906 WP_039272396 hypothetical protein -

Host bacterium

ID   15494 GenBank   NZ_OW849492
Plasmid name   NZ_OW849492 Incompatibility group   IncA/C
Plasmid size   194555 bp Coordinate of oriT [Strand]   143555..143705 [+]
Host baterium   Serratia marcescens isolate 0

Cargo genes

Drug resistance gene   blaSHV-12, tet(A), aadA2, ant(2'')-Ia
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -