Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115056 |
| Name | oriT_NZ_OW849156  |
| Organism | Serratia marcescens isolate 0 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OW849156 (45256..45361 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_NZ_OW849156
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 9636 | GenBank | WP_004187323 |
| Name | traI_LQ123_RS26335_NZ_OW849156 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 11242 | GenBank | WP_004206885 |
| Name | t4cp2_LQ123_RS25990_NZ_OW849156 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 48157..67446
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LQ123_RS26315 (AI2618V1_5033) | 43241..43552 | + | 312 | WP_004187333 | hypothetical protein | - |
| LQ123_RS26320 (AI2618V1_5034) | 43685..44221 | + | 537 | WP_004187332 | hypothetical protein | - |
| LQ123_RS26325 (AI2618V1_5035) | 44723..45118 | - | 396 | WP_019725163 | hypothetical protein | - |
| LQ123_RS26330 (AI2618V1_5036) | 45153..45680 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| LQ123_RS26335 (AI2618V1_5037) | 45667..47646 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| LQ123_RS26340 (AI2618V1_5038) | 47660..48160 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| LQ123_RS26345 (AI2618V1_5039) | 48157..48936 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| LQ123_RS26350 (AI2618V1_5040) | 48947..50110 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| LQ123_RS26355 (AI2618V1_5041) | 50100..50360 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| LQ123_RS26360 (AI2618V1_5042) | 50385..53822 | + | 3438 | WP_225373171 | LPD7 domain-containing protein | - |
| LQ123_RS26365 (AI2618V1_5043) | 53788..54300 | + | 513 | WP_011091071 | hypothetical protein | traL |
| LQ123_RS26370 | 54301..54513 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| LQ123_RS26375 | 54485..54895 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| LQ123_RS26380 (AI2618V1_5045) | 54963..55676 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| LQ123_RS26385 (AI2618V1_5046) | 55685..56836 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| LQ123_RS26390 (AI2618V1_5047) | 56848..58197 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| LQ123_RS26395 (AI2618V1_5048) | 58209..58913 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| LQ123_RS26400 (AI2618V1_5049) | 58937..59467 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| LQ123_RS26405 (AI2618V1_5050) | 59484..59873 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| LQ123_RS26410 (AI2618V1_5051) | 59919..60413 | + | 495 | WP_004187480 | hypothetical protein | - |
| LQ123_RS26415 (AI2618V1_5052) | 60410..63460 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| LQ123_RS26420 (AI2618V1_5053) | 63457..64665 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| LQ123_RS26425 (AI2618V1_5054) | 64662..65258 | + | 597 | WP_015060003 | hypothetical protein | - |
| LQ123_RS26430 (AI2618V1_5055) | 65251..67446 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| LQ123_RS26435 (AI2618V1_5056) | 67448..68101 | + | 654 | WP_015060005 | hypothetical protein | - |
| LQ123_RS26440 (AI2618V1_5057) | 68182..68412 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 15491 | GenBank | NZ_OW849156 |
| Plasmid name | NZ_OW849156 | Incompatibility group | IncL/M |
| Plasmid size | 68707 bp | Coordinate of oriT [Strand] | 45256..45361 [+] |
| Host baterium | Serratia marcescens isolate 0 |
Cargo genes
| Drug resistance gene | catA1, blaVIM-1, dfrB1, ant(3'')-Ia, catB2, qacE, sul1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |