Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 115026 |
| Name | oriT_K15/93|unnamed2  |
| Organism | Rhizobium sp. K15/93 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP072155 (248128..248164 [+], 37 nt) |
| oriT length | 37 nt |
| IRs (inverted repeats) | 22..27, 32..37 (CGTCGC..GCGACG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 37 nt
>oriT_K15/93|unnamed2
GGTTCCAAGGGCGCAATTATACGTCGCTGACGCGACG
GGTTCCAAGGGCGCAATTATACGTCGCTGACGCGACG
Visualization of oriT structure
Relaxase
| ID | 9620 | GenBank | WP_209186698 |
| Name | traA_J5280_RS31490_K15/93|unnamed2 |
UniProt ID | _ |
| Length | 1100 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1100 a.a. Molecular weight: 122896.23 Da Isoelectric Point: 10.0197
>WP_209186698.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [unclassified Rhizobium]
MAIAHFSASIVSRGDGRSALLSAAYRHCAKMEYEREARTIDYTRKQGLLHEEFVLPADAPKWARAMIADR
SVSGAVEAFWNKVEAFEKRSDAQFARDVTIALPLELTPEQNIALVRDFVERHILAKGMVADWVYHDNPGN
PHIHLMTTLRPLTDAGFGAKKVAVTGEDGQPLRTKAGKIAYELWAGSNDDFNKLREGWFERQNHHLALGG
IDLRIDGRSYEMQGIELEPTIHLGVGAKAIARKAESKGVRPELERIELNEQRRSENARRILRSPGTVLDL
ITGEKSVFDERDVAKVLLRYIDDPGIFQQLLARILQSPETLRLQRDIIEFATGQRLPSRYTTRELIRLEA
EIARRSLWLSERDTHGVSKTVLKTTFARHARLSDEQRSAIEHVAGSARIAAVVGRAGAGKTTMMKAAREA
WELAGYRVVGGALAGKAAEGLEKEAGIQSRTLASWELRWKKDRDTLDARTVFVMDEAGMVASRQMAGFVE
AVVRSGAKLVLVGDPEQLQPIEAGAAFRAIADRVGYAELETIYRQREDWMRKASLDLARGRVGEALAAYR
SEGRVLGSELKAKAVEHLIANWNRDYDPEKSMLMLAHLRRDVRMLNVMAREKLVERGIVSEGHAFRTADG
IRHFDAGDQIVFLKNEGSLGVKNGMIGRVVEAAPNRISVVVGEGDQRRQVSVEQRFYANLDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHHEDLALYYGRRSFSKAGGLTEILSRRNTKETTLDYERGSLY
RPALAFAENRGLHIVQVARTLLRDHLDWTLRQSSKLADLATRLRAAGERLGLLQTLKSQTNKETRPMVSG
VKLFPVPLSDAVDRKVADDPAVKKQWEEVSTRFRYVFVDPETAFRAINFDAVLADKKVAVQTLDKLAADP
ASTGPLKGRTGILASKSDREARRVAEVNVPALKRDIETYLRIREAAVQRIEGDEKTQRQRVAIDIPALSP
AAQSVLERVRDAIDRNDLPAAMAYALSNCETKSEIAGFSKAVTERFGERTLLTNTAREPQGQLFDKLSEG
LAPQEKARLKEAWPVMRTAQQLATHERTVAKLSQAEDLKLVQRQTPVIKQ
MAIAHFSASIVSRGDGRSALLSAAYRHCAKMEYEREARTIDYTRKQGLLHEEFVLPADAPKWARAMIADR
SVSGAVEAFWNKVEAFEKRSDAQFARDVTIALPLELTPEQNIALVRDFVERHILAKGMVADWVYHDNPGN
PHIHLMTTLRPLTDAGFGAKKVAVTGEDGQPLRTKAGKIAYELWAGSNDDFNKLREGWFERQNHHLALGG
IDLRIDGRSYEMQGIELEPTIHLGVGAKAIARKAESKGVRPELERIELNEQRRSENARRILRSPGTVLDL
ITGEKSVFDERDVAKVLLRYIDDPGIFQQLLARILQSPETLRLQRDIIEFATGQRLPSRYTTRELIRLEA
EIARRSLWLSERDTHGVSKTVLKTTFARHARLSDEQRSAIEHVAGSARIAAVVGRAGAGKTTMMKAAREA
WELAGYRVVGGALAGKAAEGLEKEAGIQSRTLASWELRWKKDRDTLDARTVFVMDEAGMVASRQMAGFVE
AVVRSGAKLVLVGDPEQLQPIEAGAAFRAIADRVGYAELETIYRQREDWMRKASLDLARGRVGEALAAYR
SEGRVLGSELKAKAVEHLIANWNRDYDPEKSMLMLAHLRRDVRMLNVMAREKLVERGIVSEGHAFRTADG
IRHFDAGDQIVFLKNEGSLGVKNGMIGRVVEAAPNRISVVVGEGDQRRQVSVEQRFYANLDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHHEDLALYYGRRSFSKAGGLTEILSRRNTKETTLDYERGSLY
RPALAFAENRGLHIVQVARTLLRDHLDWTLRQSSKLADLATRLRAAGERLGLLQTLKSQTNKETRPMVSG
VKLFPVPLSDAVDRKVADDPAVKKQWEEVSTRFRYVFVDPETAFRAINFDAVLADKKVAVQTLDKLAADP
ASTGPLKGRTGILASKSDREARRVAEVNVPALKRDIETYLRIREAAVQRIEGDEKTQRQRVAIDIPALSP
AAQSVLERVRDAIDRNDLPAAMAYALSNCETKSEIAGFSKAVTERFGERTLLTNTAREPQGQLFDKLSEG
LAPQEKARLKEAWPVMRTAQQLATHERTVAKLSQAEDLKLVQRQTPVIKQ
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 11224 | GenBank | WP_247749463 |
| Name | traG_J5280_RS31475_K15/93|unnamed2 |
UniProt ID | _ |
| Length | 658 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 658 a.a. Molecular weight: 70152.20 Da Isoelectric Point: 9.6846
>WP_247749463.1 MULTISPECIES: Ti-type conjugative transfer system protein TraG [unclassified Rhizobium]
MTANRLLLGTLPAIIMLAVMFGTTGMEHRLATWGTSAQAKLLLGRAGLVLPYIAAAAIGVISLFAAYGSA
NIKTAALSVLAGNGAVMVVAVVREAIRLAGIASSVPAGQSALAYTDPATAAGASAALFCGVFAMRVAIKG
NAAFAQGGPTRVSGKRAVHGDAHWMSMAEAAKLFPDAGGIVVGELYRVDRDGVAAMAFRTGNPQSWGAGG
KSPLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGGLVVLDPSSEVAPMVVEHRRKAGRKVIVLD
PASPATGFNALDWIGRFGGTKEEDIVAVATWIMTDNARTASARDDFFRASAMQLLTALIADVCLSGHTDA
KDQTLRRVRANLSEPEPKLRERLTRIYEQSESTFVRENVAVFVNMTPETFSGVYANAVKETHWLSYTNYA
ALVSGDSFTTDELATGITDIFIAFDLKVMESHPGLARVVIGSFLNAIYNRNGEVAGRTLFLLDEVARLGY
LRILETARDAGRKYGISLALVFQSIGQMREAYGGRDASSKWFESASWISFAAINDPETAEYISKRCGDTT
VEVDQTNRSSGMKGSSRSRSKQLTRRPLILPHEVLRMRGDEQIVFTAGNAPLRCGRAIWFRRKDMSASVG
ENRFHRQAIKGSGANETGPATGIEKITD
MTANRLLLGTLPAIIMLAVMFGTTGMEHRLATWGTSAQAKLLLGRAGLVLPYIAAAAIGVISLFAAYGSA
NIKTAALSVLAGNGAVMVVAVVREAIRLAGIASSVPAGQSALAYTDPATAAGASAALFCGVFAMRVAIKG
NAAFAQGGPTRVSGKRAVHGDAHWMSMAEAAKLFPDAGGIVVGELYRVDRDGVAAMAFRTGNPQSWGAGG
KSPLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGGLVVLDPSSEVAPMVVEHRRKAGRKVIVLD
PASPATGFNALDWIGRFGGTKEEDIVAVATWIMTDNARTASARDDFFRASAMQLLTALIADVCLSGHTDA
KDQTLRRVRANLSEPEPKLRERLTRIYEQSESTFVRENVAVFVNMTPETFSGVYANAVKETHWLSYTNYA
ALVSGDSFTTDELATGITDIFIAFDLKVMESHPGLARVVIGSFLNAIYNRNGEVAGRTLFLLDEVARLGY
LRILETARDAGRKYGISLALVFQSIGQMREAYGGRDASSKWFESASWISFAAINDPETAEYISKRCGDTT
VEVDQTNRSSGMKGSSRSRSKQLTRRPLILPHEVLRMRGDEQIVFTAGNAPLRCGRAIWFRRKDMSASVG
ENRFHRQAIKGSGANETGPATGIEKITD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 11225 | GenBank | WP_209186672 |
| Name | t4cp2_J5280_RS31625_K15/93|unnamed2 |
UniProt ID | _ |
| Length | 820 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 820 a.a. Molecular weight: 91600.37 Da Isoelectric Point: 5.9910
>WP_209186672.1 MULTISPECIES: conjugal transfer protein TrbE [unclassified Rhizobium]
MVALKPFRHSGPSFADLAPYAGLVDNGVVLLKDGSLMAGWYFAGPDSESSTDAERNEVSRQINAILSRLG
SGWMIQVEAIRVPTGDYPTEAESHFTDPVTRAIDDERRAHFQKERGHFESRHALILTWRPPEPRRSGLTR
YVYSDAASRSATYADTMLQSFLTKIREVEQYLANVLSIRRMMTREKSERGGFRVARYDELFQFIRFCITG
ENHPVRLPEIPMYLDWLVTAELQHGLTPMVENRFLGLVAIDGLPAESWPGILNTLDLMPLTYRWSSRFLF
LDAEEARARLERTRKKWQQKVRPFFDQLFQTQSRSMDQDAMLMVAETEDAIAEASSQLVAYGYYTPVIVL
FEEDQHRLQEKCEAVRRLIQAEGFGARIETLNATDAFLGSLPGVSYANIREPLVNTRNLADLIPLNSVWS
GSPNAPCPFYPQGSPPLMQVASGSTAFCLNLHVDDVGHTLAFGPTGSGKSTLLALIAAQFRRYADAQIFA
FDKGGSMLPLTLGVDGDHYQIGGGATGEGEGRALAFCPLAELSSDGDRAWATEWIETLVALQGVTITPDY
RNAISRQIALMATSRARSLSDFVSGVQMREIKDALHHYTVDGSMGQLLDAEQDGLALGSFQCFEIEELMN
MGERNLVPVLTYLFRRIEKRLTGAPSLIILDEAWLMLGHPVFRDKIREWLKVLRKANCAVVLATQSISDA
ERSGIIDVLKESCPTKICLPNGAAREPGTREFYERIGFNERQIEIVATAIPKRDYYVVSPEGRRLFNMAL
GPVALSFVGATGKEDLKRIRSLHSEHGAAWPLYWLQQRGIAHADTLISTS
MVALKPFRHSGPSFADLAPYAGLVDNGVVLLKDGSLMAGWYFAGPDSESSTDAERNEVSRQINAILSRLG
SGWMIQVEAIRVPTGDYPTEAESHFTDPVTRAIDDERRAHFQKERGHFESRHALILTWRPPEPRRSGLTR
YVYSDAASRSATYADTMLQSFLTKIREVEQYLANVLSIRRMMTREKSERGGFRVARYDELFQFIRFCITG
ENHPVRLPEIPMYLDWLVTAELQHGLTPMVENRFLGLVAIDGLPAESWPGILNTLDLMPLTYRWSSRFLF
LDAEEARARLERTRKKWQQKVRPFFDQLFQTQSRSMDQDAMLMVAETEDAIAEASSQLVAYGYYTPVIVL
FEEDQHRLQEKCEAVRRLIQAEGFGARIETLNATDAFLGSLPGVSYANIREPLVNTRNLADLIPLNSVWS
GSPNAPCPFYPQGSPPLMQVASGSTAFCLNLHVDDVGHTLAFGPTGSGKSTLLALIAAQFRRYADAQIFA
FDKGGSMLPLTLGVDGDHYQIGGGATGEGEGRALAFCPLAELSSDGDRAWATEWIETLVALQGVTITPDY
RNAISRQIALMATSRARSLSDFVSGVQMREIKDALHHYTVDGSMGQLLDAEQDGLALGSFQCFEIEELMN
MGERNLVPVLTYLFRRIEKRLTGAPSLIILDEAWLMLGHPVFRDKIREWLKVLRKANCAVVLATQSISDA
ERSGIIDVLKESCPTKICLPNGAAREPGTREFYERIGFNERQIEIVATAIPKRDYYVVSPEGRRLFNMAL
GPVALSFVGATGKEDLKRIRSLHSEHGAAWPLYWLQQRGIAHADTLISTS
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 271296..280964
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J5280_RS31565 (J5280_31520) | 266951..268210 | + | 1260 | WP_209186701 | peptidase T | - |
| J5280_RS31570 (J5280_31525) | 268288..268554 | - | 267 | WP_209186661 | helix-turn-helix domain-containing protein | - |
| J5280_RS31575 (J5280_31530) | 268791..269099 | + | 309 | WP_209186662 | transcriptional repressor TraM | - |
| J5280_RS31580 (J5280_31535) | 269109..269813 | - | 705 | WP_209186663 | transcriptional regulator TraR | - |
| J5280_RS31585 (J5280_31540) | 270323..271273 | + | 951 | WP_209186664 | LysR family transcriptional regulator | - |
| J5280_RS31590 (J5280_31545) | 271296..272612 | - | 1317 | WP_209186665 | IncP-type conjugal transfer protein TrbI | virB10 |
| J5280_RS31595 (J5280_31550) | 272624..273100 | - | 477 | WP_209186666 | conjugal transfer protein TrbH | - |
| J5280_RS31600 (J5280_31555) | 273100..273921 | - | 822 | WP_247749466 | P-type conjugative transfer protein TrbG | virB9 |
| J5280_RS31605 (J5280_31560) | 273969..274646 | - | 678 | WP_209186668 | conjugal transfer protein TrbF | virB8 |
| J5280_RS31610 (J5280_31565) | 274664..275854 | - | 1191 | WP_209186669 | P-type conjugative transfer protein TrbL | virB6 |
| J5280_RS31615 (J5280_31570) | 275848..276060 | - | 213 | WP_209186670 | entry exclusion protein TrbK | - |
| J5280_RS31620 (J5280_31575) | 276057..276863 | - | 807 | WP_209186671 | P-type conjugative transfer protein TrbJ | virB5 |
| J5280_RS31625 (J5280_31580) | 276835..279297 | - | 2463 | WP_209186672 | conjugal transfer protein TrbE | virb4 |
| J5280_RS31630 (J5280_31585) | 279307..279606 | - | 300 | WP_209186673 | conjugal transfer protein TrbD | virB3 |
| J5280_RS31635 (J5280_31590) | 279599..280003 | - | 405 | WP_209186674 | conjugal transfer pilin TrbC | virB2 |
| J5280_RS31640 (J5280_31595) | 279993..280964 | - | 972 | WP_209186675 | P-type conjugative transfer ATPase TrbB | virB11 |
| J5280_RS31645 (J5280_31600) | 280961..281599 | - | 639 | WP_209186676 | acyl-homoserine-lactone synthase TraI | - |
Host bacterium
| ID | 15461 | GenBank | NZ_CP072155 |
| Plasmid name | K15/93|unnamed2 | Incompatibility group | - |
| Plasmid size | 281888 bp | Coordinate of oriT [Strand] | 248128..248164 [+] |
| Host baterium | Rhizobium sp. K15/93 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |