Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115025
Name   oriT_K1/93|unnamed2 in_silico
Organism   Rhizobium sp. K1/93
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP072150 (55823..55859 [+], 37 nt)
oriT length   37 nt
IRs (inverted repeats)      22..27, 32..37  (CGTCGC..GCGACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 37 nt

>oriT_K1/93|unnamed2
GGTTCCAAGGGCGCAATTATACGTCGCTGACGCGACG

Visualization of oriT structure



Relaxase

ID   9619 GenBank   WP_209186698
Name   traA_J5287_RS30665_K1/93|unnamed2 insolico UniProt ID   _
Length   1100 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1100 a.a.        Molecular weight: 122896.23 Da        Isoelectric Point: 10.0197

>WP_209186698.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [unclassified Rhizobium]
MAIAHFSASIVSRGDGRSALLSAAYRHCAKMEYEREARTIDYTRKQGLLHEEFVLPADAPKWARAMIADR
SVSGAVEAFWNKVEAFEKRSDAQFARDVTIALPLELTPEQNIALVRDFVERHILAKGMVADWVYHDNPGN
PHIHLMTTLRPLTDAGFGAKKVAVTGEDGQPLRTKAGKIAYELWAGSNDDFNKLREGWFERQNHHLALGG
IDLRIDGRSYEMQGIELEPTIHLGVGAKAIARKAESKGVRPELERIELNEQRRSENARRILRSPGTVLDL
ITGEKSVFDERDVAKVLLRYIDDPGIFQQLLARILQSPETLRLQRDIIEFATGQRLPSRYTTRELIRLEA
EIARRSLWLSERDTHGVSKTVLKTTFARHARLSDEQRSAIEHVAGSARIAAVVGRAGAGKTTMMKAAREA
WELAGYRVVGGALAGKAAEGLEKEAGIQSRTLASWELRWKKDRDTLDARTVFVMDEAGMVASRQMAGFVE
AVVRSGAKLVLVGDPEQLQPIEAGAAFRAIADRVGYAELETIYRQREDWMRKASLDLARGRVGEALAAYR
SEGRVLGSELKAKAVEHLIANWNRDYDPEKSMLMLAHLRRDVRMLNVMAREKLVERGIVSEGHAFRTADG
IRHFDAGDQIVFLKNEGSLGVKNGMIGRVVEAAPNRISVVVGEGDQRRQVSVEQRFYANLDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHHEDLALYYGRRSFSKAGGLTEILSRRNTKETTLDYERGSLY
RPALAFAENRGLHIVQVARTLLRDHLDWTLRQSSKLADLATRLRAAGERLGLLQTLKSQTNKETRPMVSG
VKLFPVPLSDAVDRKVADDPAVKKQWEEVSTRFRYVFVDPETAFRAINFDAVLADKKVAVQTLDKLAADP
ASTGPLKGRTGILASKSDREARRVAEVNVPALKRDIETYLRIREAAVQRIEGDEKTQRQRVAIDIPALSP
AAQSVLERVRDAIDRNDLPAAMAYALSNCETKSEIAGFSKAVTERFGERTLLTNTAREPQGQLFDKLSEG
LAPQEKARLKEAWPVMRTAQQLATHERTVAKLSQAEDLKLVQRQTPVIKQ

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(382-565)

MobA/MobL family (MobA_MobL)

(17-243)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(855-951)


  Protein structure



No available structure.




T4CP

ID   11222 GenBank   WP_247749463
Name   traG_J5287_RS30650_K1/93|unnamed2 insolico UniProt ID   _
Length   658 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 658 a.a.        Molecular weight: 70152.20 Da        Isoelectric Point: 9.6846

>WP_247749463.1 MULTISPECIES: Ti-type conjugative transfer system protein TraG [unclassified Rhizobium]
MTANRLLLGTLPAIIMLAVMFGTTGMEHRLATWGTSAQAKLLLGRAGLVLPYIAAAAIGVISLFAAYGSA
NIKTAALSVLAGNGAVMVVAVVREAIRLAGIASSVPAGQSALAYTDPATAAGASAALFCGVFAMRVAIKG
NAAFAQGGPTRVSGKRAVHGDAHWMSMAEAAKLFPDAGGIVVGELYRVDRDGVAAMAFRTGNPQSWGAGG
KSPLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGGLVVLDPSSEVAPMVVEHRRKAGRKVIVLD
PASPATGFNALDWIGRFGGTKEEDIVAVATWIMTDNARTASARDDFFRASAMQLLTALIADVCLSGHTDA
KDQTLRRVRANLSEPEPKLRERLTRIYEQSESTFVRENVAVFVNMTPETFSGVYANAVKETHWLSYTNYA
ALVSGDSFTTDELATGITDIFIAFDLKVMESHPGLARVVIGSFLNAIYNRNGEVAGRTLFLLDEVARLGY
LRILETARDAGRKYGISLALVFQSIGQMREAYGGRDASSKWFESASWISFAAINDPETAEYISKRCGDTT
VEVDQTNRSSGMKGSSRSRSKQLTRRPLILPHEVLRMRGDEQIVFTAGNAPLRCGRAIWFRRKDMSASVG
ENRFHRQAIKGSGANETGPATGIEKITD

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(164-640)

  Protein structure



No available structure.



ID   11223 GenBank   WP_209186672
Name   t4cp2_J5287_RS30800_K1/93|unnamed2 insolico UniProt ID   _
Length   820 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 820 a.a.        Molecular weight: 91600.37 Da        Isoelectric Point: 5.9910

>WP_209186672.1 MULTISPECIES: conjugal transfer protein TrbE [unclassified Rhizobium]
MVALKPFRHSGPSFADLAPYAGLVDNGVVLLKDGSLMAGWYFAGPDSESSTDAERNEVSRQINAILSRLG
SGWMIQVEAIRVPTGDYPTEAESHFTDPVTRAIDDERRAHFQKERGHFESRHALILTWRPPEPRRSGLTR
YVYSDAASRSATYADTMLQSFLTKIREVEQYLANVLSIRRMMTREKSERGGFRVARYDELFQFIRFCITG
ENHPVRLPEIPMYLDWLVTAELQHGLTPMVENRFLGLVAIDGLPAESWPGILNTLDLMPLTYRWSSRFLF
LDAEEARARLERTRKKWQQKVRPFFDQLFQTQSRSMDQDAMLMVAETEDAIAEASSQLVAYGYYTPVIVL
FEEDQHRLQEKCEAVRRLIQAEGFGARIETLNATDAFLGSLPGVSYANIREPLVNTRNLADLIPLNSVWS
GSPNAPCPFYPQGSPPLMQVASGSTAFCLNLHVDDVGHTLAFGPTGSGKSTLLALIAAQFRRYADAQIFA
FDKGGSMLPLTLGVDGDHYQIGGGATGEGEGRALAFCPLAELSSDGDRAWATEWIETLVALQGVTITPDY
RNAISRQIALMATSRARSLSDFVSGVQMREIKDALHHYTVDGSMGQLLDAEQDGLALGSFQCFEIEELMN
MGERNLVPVLTYLFRRIEKRLTGAPSLIILDEAWLMLGHPVFRDKIREWLKVLRKANCAVVLATQSISDA
ERSGIIDVLKESCPTKICLPNGAAREPGTREFYERIGFNERQIEIVATAIPKRDYYVVSPEGRRLFNMAL
GPVALSFVGATGKEDLKRIRSLHSEHGAAWPLYWLQQRGIAHADTLISTS

  Protein domains


Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(188-400)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 78991..88659

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5287_RS30740 (J5287_30710) 74646..75905 + 1260 WP_209186701 peptidase T -
J5287_RS30745 (J5287_30715) 75983..76249 - 267 WP_209186661 helix-turn-helix domain-containing protein -
J5287_RS30750 (J5287_30720) 76486..76794 + 309 WP_209186662 transcriptional repressor TraM -
J5287_RS30755 (J5287_30725) 76804..77508 - 705 WP_209186663 transcriptional regulator TraR -
J5287_RS30760 (J5287_30730) 78018..78968 + 951 WP_209186664 LysR family transcriptional regulator -
J5287_RS30765 (J5287_30735) 78991..80307 - 1317 WP_209186665 IncP-type conjugal transfer protein TrbI virB10
J5287_RS30770 (J5287_30740) 80319..80795 - 477 WP_209186666 conjugal transfer protein TrbH -
J5287_RS30775 (J5287_30745) 80795..81616 - 822 WP_247749466 P-type conjugative transfer protein TrbG virB9
J5287_RS30780 (J5287_30750) 81664..82341 - 678 WP_209186668 conjugal transfer protein TrbF virB8
J5287_RS30785 (J5287_30755) 82359..83549 - 1191 WP_209186669 P-type conjugative transfer protein TrbL virB6
J5287_RS30790 (J5287_30760) 83543..83755 - 213 WP_209186670 entry exclusion protein TrbK -
J5287_RS30795 (J5287_30765) 83752..84558 - 807 WP_209186671 P-type conjugative transfer protein TrbJ virB5
J5287_RS30800 (J5287_30770) 84530..86992 - 2463 WP_209186672 conjugal transfer protein TrbE virb4
J5287_RS30805 (J5287_30775) 87002..87301 - 300 WP_209186673 conjugal transfer protein TrbD virB3
J5287_RS30810 (J5287_30780) 87294..87698 - 405 WP_209186674 conjugal transfer pilin TrbC virB2
J5287_RS30815 (J5287_30785) 87688..88659 - 972 WP_209186675 P-type conjugative transfer ATPase TrbB virB11
J5287_RS30820 (J5287_30790) 88656..89294 - 639 WP_209186676 acyl-homoserine-lactone synthase TraI -
J5287_RS30825 (J5287_30795) 89650..90858 + 1209 WP_209186702 plasmid partitioning protein RepA -
J5287_RS30830 (J5287_30800) 90962..91966 + 1005 WP_209186677 plasmid partitioning protein RepB -
J5287_RS30835 (J5287_30805) 92135..93346 + 1212 WP_209186678 plasmid replication protein RepC -

Host bacterium

ID   15460 GenBank   NZ_CP072150
Plasmid name   K1/93|unnamed2 Incompatibility group   -
Plasmid size   281888 bp Coordinate of oriT [Strand]   55823..55859 [+]
Host baterium   Rhizobium sp. K1/93

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -