Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115024
Name   oriT_B21/90|unnamed2 in_silico
Organism   Rhizobium sp. B21/90
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP072166 (206296..206332 [-], 37 nt)
oriT length   37 nt
IRs (inverted repeats)      22..27, 32..37  (CGTCGC..GCGACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 37 nt

>oriT_B21/90|unnamed2
GGTTCCAAGGGCGCAATTATACGTCGCTGACGCGACG

Visualization of oriT structure



Relaxase

ID   9618 GenBank   WP_209186698
Name   traA_J5278_RS30475_B21/90|unnamed2 insolico UniProt ID   _
Length   1100 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1100 a.a.        Molecular weight: 122896.23 Da        Isoelectric Point: 10.0197

>WP_209186698.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [unclassified Rhizobium]
MAIAHFSASIVSRGDGRSALLSAAYRHCAKMEYEREARTIDYTRKQGLLHEEFVLPADAPKWARAMIADR
SVSGAVEAFWNKVEAFEKRSDAQFARDVTIALPLELTPEQNIALVRDFVERHILAKGMVADWVYHDNPGN
PHIHLMTTLRPLTDAGFGAKKVAVTGEDGQPLRTKAGKIAYELWAGSNDDFNKLREGWFERQNHHLALGG
IDLRIDGRSYEMQGIELEPTIHLGVGAKAIARKAESKGVRPELERIELNEQRRSENARRILRSPGTVLDL
ITGEKSVFDERDVAKVLLRYIDDPGIFQQLLARILQSPETLRLQRDIIEFATGQRLPSRYTTRELIRLEA
EIARRSLWLSERDTHGVSKTVLKTTFARHARLSDEQRSAIEHVAGSARIAAVVGRAGAGKTTMMKAAREA
WELAGYRVVGGALAGKAAEGLEKEAGIQSRTLASWELRWKKDRDTLDARTVFVMDEAGMVASRQMAGFVE
AVVRSGAKLVLVGDPEQLQPIEAGAAFRAIADRVGYAELETIYRQREDWMRKASLDLARGRVGEALAAYR
SEGRVLGSELKAKAVEHLIANWNRDYDPEKSMLMLAHLRRDVRMLNVMAREKLVERGIVSEGHAFRTADG
IRHFDAGDQIVFLKNEGSLGVKNGMIGRVVEAAPNRISVVVGEGDQRRQVSVEQRFYANLDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHHEDLALYYGRRSFSKAGGLTEILSRRNTKETTLDYERGSLY
RPALAFAENRGLHIVQVARTLLRDHLDWTLRQSSKLADLATRLRAAGERLGLLQTLKSQTNKETRPMVSG
VKLFPVPLSDAVDRKVADDPAVKKQWEEVSTRFRYVFVDPETAFRAINFDAVLADKKVAVQTLDKLAADP
ASTGPLKGRTGILASKSDREARRVAEVNVPALKRDIETYLRIREAAVQRIEGDEKTQRQRVAIDIPALSP
AAQSVLERVRDAIDRNDLPAAMAYALSNCETKSEIAGFSKAVTERFGERTLLTNTAREPQGQLFDKLSEG
LAPQEKARLKEAWPVMRTAQQLATHERTVAKLSQAEDLKLVQRQTPVIKQ

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(382-565)

MobA/MobL family (MobA_MobL)

(17-243)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(855-951)


  Protein structure



No available structure.




T4CP

ID   11220 GenBank   WP_209186672
Name   t4cp2_J5278_RS30340_B21/90|unnamed2 insolico UniProt ID   _
Length   820 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 820 a.a.        Molecular weight: 91600.37 Da        Isoelectric Point: 5.9910

>WP_209186672.1 MULTISPECIES: conjugal transfer protein TrbE [unclassified Rhizobium]
MVALKPFRHSGPSFADLAPYAGLVDNGVVLLKDGSLMAGWYFAGPDSESSTDAERNEVSRQINAILSRLG
SGWMIQVEAIRVPTGDYPTEAESHFTDPVTRAIDDERRAHFQKERGHFESRHALILTWRPPEPRRSGLTR
YVYSDAASRSATYADTMLQSFLTKIREVEQYLANVLSIRRMMTREKSERGGFRVARYDELFQFIRFCITG
ENHPVRLPEIPMYLDWLVTAELQHGLTPMVENRFLGLVAIDGLPAESWPGILNTLDLMPLTYRWSSRFLF
LDAEEARARLERTRKKWQQKVRPFFDQLFQTQSRSMDQDAMLMVAETEDAIAEASSQLVAYGYYTPVIVL
FEEDQHRLQEKCEAVRRLIQAEGFGARIETLNATDAFLGSLPGVSYANIREPLVNTRNLADLIPLNSVWS
GSPNAPCPFYPQGSPPLMQVASGSTAFCLNLHVDDVGHTLAFGPTGSGKSTLLALIAAQFRRYADAQIFA
FDKGGSMLPLTLGVDGDHYQIGGGATGEGEGRALAFCPLAELSSDGDRAWATEWIETLVALQGVTITPDY
RNAISRQIALMATSRARSLSDFVSGVQMREIKDALHHYTVDGSMGQLLDAEQDGLALGSFQCFEIEELMN
MGERNLVPVLTYLFRRIEKRLTGAPSLIILDEAWLMLGHPVFRDKIREWLKVLRKANCAVVLATQSISDA
ERSGIIDVLKESCPTKICLPNGAAREPGTREFYERIGFNERQIEIVATAIPKRDYYVVSPEGRRLFNMAL
GPVALSFVGATGKEDLKRIRSLHSEHGAAWPLYWLQQRGIAHADTLISTS

  Protein domains


Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(188-400)

  Protein structure



No available structure.



ID   11221 GenBank   WP_247749463
Name   traG_J5278_RS30490_B21/90|unnamed2 insolico UniProt ID   _
Length   658 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 658 a.a.        Molecular weight: 70152.20 Da        Isoelectric Point: 9.6846

>WP_247749463.1 MULTISPECIES: Ti-type conjugative transfer system protein TraG [unclassified Rhizobium]
MTANRLLLGTLPAIIMLAVMFGTTGMEHRLATWGTSAQAKLLLGRAGLVLPYIAAAAIGVISLFAAYGSA
NIKTAALSVLAGNGAVMVVAVVREAIRLAGIASSVPAGQSALAYTDPATAAGASAALFCGVFAMRVAIKG
NAAFAQGGPTRVSGKRAVHGDAHWMSMAEAAKLFPDAGGIVVGELYRVDRDGVAAMAFRTGNPQSWGAGG
KSPLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGGLVVLDPSSEVAPMVVEHRRKAGRKVIVLD
PASPATGFNALDWIGRFGGTKEEDIVAVATWIMTDNARTASARDDFFRASAMQLLTALIADVCLSGHTDA
KDQTLRRVRANLSEPEPKLRERLTRIYEQSESTFVRENVAVFVNMTPETFSGVYANAVKETHWLSYTNYA
ALVSGDSFTTDELATGITDIFIAFDLKVMESHPGLARVVIGSFLNAIYNRNGEVAGRTLFLLDEVARLGY
LRILETARDAGRKYGISLALVFQSIGQMREAYGGRDASSKWFESASWISFAAINDPETAEYISKRCGDTT
VEVDQTNRSSGMKGSSRSRSKQLTRRPLILPHEVLRMRGDEQIVFTAGNAPLRCGRAIWFRRKDMSASVG
ENRFHRQAIKGSGANETGPATGIEKITD

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(164-640)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 173497..183165

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5278_RS30305 (J5278_30305) 168810..170021 - 1212 WP_209186678 plasmid replication protein RepC -
J5278_RS30310 (J5278_30310) 170190..171194 - 1005 WP_209186677 plasmid partitioning protein RepB -
J5278_RS30315 (J5278_30315) 171298..172506 - 1209 WP_209186702 plasmid partitioning protein RepA -
J5278_RS30320 (J5278_30320) 172862..173500 + 639 WP_209186676 acyl-homoserine-lactone synthase TraI -
J5278_RS30325 (J5278_30325) 173497..174468 + 972 WP_209186675 P-type conjugative transfer ATPase TrbB virB11
J5278_RS30330 (J5278_30330) 174458..174862 + 405 WP_209186674 conjugal transfer pilin TrbC virB2
J5278_RS30335 (J5278_30335) 174855..175154 + 300 WP_209186673 conjugal transfer protein TrbD virB3
J5278_RS30340 (J5278_30340) 175164..177626 + 2463 WP_209186672 conjugal transfer protein TrbE virb4
J5278_RS30345 (J5278_30345) 177598..178404 + 807 WP_209186671 P-type conjugative transfer protein TrbJ virB5
J5278_RS30350 (J5278_30350) 178401..178613 + 213 WP_209186670 entry exclusion protein TrbK -
J5278_RS30355 (J5278_30355) 178607..179797 + 1191 WP_209186669 P-type conjugative transfer protein TrbL virB6
J5278_RS30360 (J5278_30360) 179815..180492 + 678 WP_209186668 conjugal transfer protein TrbF virB8
J5278_RS30365 (J5278_30365) 180540..181361 + 822 WP_247749466 P-type conjugative transfer protein TrbG virB9
J5278_RS30370 (J5278_30370) 181361..181837 + 477 WP_209186666 conjugal transfer protein TrbH -
J5278_RS30375 (J5278_30375) 181849..183165 + 1317 WP_209186665 IncP-type conjugal transfer protein TrbI virB10
J5278_RS30380 (J5278_30380) 183188..184138 - 951 WP_209186664 LysR family transcriptional regulator -
J5278_RS30385 (J5278_30385) 184648..185352 + 705 WP_209186663 transcriptional regulator TraR -
J5278_RS30390 (J5278_30390) 185362..185670 - 309 WP_209186662 transcriptional repressor TraM -
J5278_RS30395 (J5278_30395) 185907..186173 + 267 WP_209186661 helix-turn-helix domain-containing protein -
J5278_RS30400 (J5278_30400) 186251..187510 - 1260 WP_209186701 peptidase T -

Host bacterium

ID   15459 GenBank   NZ_CP072166
Plasmid name   B21/90|unnamed2 Incompatibility group   -
Plasmid size   276662 bp Coordinate of oriT [Strand]   206296..206332 [-]
Host baterium   Rhizobium sp. B21/90

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -