Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   115023
Name   oriT_B230/85|unnamed2 in_silico
Organism   Rhizobium sp. B230/85
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP072159 (180109..180145 [+], 37 nt)
oriT length   37 nt
IRs (inverted repeats)      22..27, 32..37  (CGTCGT..ACGACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 37 nt

>oriT_B230/85|unnamed2
GGTTCCAAGGGCGCAGTTATACGTCGTTGACACGACG

Visualization of oriT structure



Relaxase

ID   9617 GenBank   WP_209207936
Name   traA_J5289_RS27530_B230/85|unnamed2 insolico UniProt ID   _
Length   1100 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1100 a.a.        Molecular weight: 123284.78 Da        Isoelectric Point: 9.7171

>WP_209207936.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [unclassified Rhizobium]
MAIAHFSAGIVSRGDGRIAVLSAAYRHCAKMEYEREARTIDYTLKQGLLHEEFVLPADAPKWARAMIADR
SVSGAVEAFWNKVETFEKRSDAQLARDLTIALPLELTPEQNIALVRDFVERHILAKGMVADWVYHDNPGN
PHIHLMTTLRPLTEDGFGAKKVAVIGEDGQPLRTNSGKIVYELWAGSTDDFNKLRDGWFERQNHHLALGG
IDLRIDGRSYEKQGIELEPTIHLGVGAKAIERKAESRGVRPELERIELNEQRRSENVRRIVRSPGIVLDL
ITREKSVFDIQDVAKVLHRYVDDPGIFQQLLARILQSPETLRLQRDTIESATGQRLPSRYTTRELIRLEA
EMARRSLWLSERDTHGVSKTVLEATFARHVRLSDEQRAAIEHVAGSARITAVVGRAGAGKTTMMKAAREA
WELGGYRVVGGALAGKAAEGLEKEAGIQSRTLASWELRWKKDRDTLDAKTVFVMDEAGMVASRQMAGFVE
AVVKTGAKLVLVGDPEQLQPIEAGAAFRAIADRVGYAELETIYRQREDWMRKASLDLARGRVGEALAAYC
SEGRVLGSELKAQAVEHLIADWNRDYDPAKSMLMLAHLRRDVRTLNVMAREKLVERGILSEGHAFRTADG
LRHFDAGDQIVFLKNEGSLGVKNGMIGCVVEATWNRISVVVGEGDHRRQVSVEQRFYNNLDHGYATTIHK
SQGATVDRVKLLASLSLDRHLTYVAMTRHREDLALYYGRRSFSKAGGLTQILSRRNAKETTLDYERSGLY
RPALAFAQNRGLHIVQVARTLLRDRLDWTLRQKSKFSDLAVRLRAVGERLGLLQTLKTQTTKETRPMVSG
VKLFPLPLSDAVDQKVANDPAVKKQWEEVSTRFRYVFADPETAFRAMNFDIVLADKQVAMQTLDKLATEP
ASIGPLKGMTGILTSKSDREARRVAEVNAPALQRDIETYLRIREAAVQRIEDEEKTMRERVSIDIPALSP
AAQTVLERVRDAIDRNDLPTAAAYALSNRETKVEIDGLNKAVAERFGERTLLANSARTPDGQLFDKLSEG
LAPQEKARLKEAWPVMRTAQQLAAHERTVTTLKQVEDLKLAQRQTPVIKQ

  Protein domains


Predicted by InterproScan.

Viral (Superfamily 1) RNA helicase (Viral_helicase1)

(692-735)

AAA domain (AAA_30)

(382-565)

MobA/MobL family (MobA_MobL)

(18-243)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(855-951)


  Protein structure



No available structure.




T4CP

ID   11218 GenBank   WP_209206909
Name   t4cp2_J5289_RS27100_B230/85|unnamed2 insolico UniProt ID   _
Length   820 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 820 a.a.        Molecular weight: 91373.07 Da        Isoelectric Point: 6.1584

>WP_209206909.1 MULTISPECIES: conjugal transfer protein TrbE [unclassified Rhizobium]
MVALKTFRHSGPSFADLAPYAGLVDNGVILLKDGSLMAGWYFAGPDSESSTDAERNEVSRQINAVLSRLG
SGWMIQVEAVRVPTGDYPDEAVSHFPDPVTRAIDNERRAHFQKERGHFESRHALILTWRPPEPRRSGLTR
YVYSDAASRSATYADTMLQGFLTSIREVEQYLSNVVSIRRMTTRETSERGGFRVARYDELFQFIRFCITG
ENHPVRLPEIPMYLDWLVTAELQHGLTPLVENRFLGVVAIDGLPAESWPGILNTLDRMPLTYRWSSRFVF
LDAEEARARLERTRKKWQQKVRPFFDQLFQTHSRSVDQDAMLMVAETEDAIAEASSQLIAYGYYTPVIVL
FEQDQHRLQEKCEAIRRLIQAEGFGARIETLNATDAFIGSLPGVSYANIREPLINTRNLADLIPLNSVWS
GSPNAPCPFYPPASPPLMQVASGSTPFRLNLHVDDVGHTLAFGPTGSGKSTLLALIAAQFRRYADAQIFA
FDKGGSMMPLTLGVGGDHYQIGGDATGEGVGGALAFCPLAELSSDGDRAWASEWIEMLVALQGVTITPDY
RNAISRQIALMATSRARSLSDFVSGVQMREIKDALHHYTVDGPVGQLLDAEQDGLALGAFQCFEIEELMN
MGERNLVPVLTYLFRRIEKRLTGAPSLIILDEAWLMLGHPVFRDKIREWLKVLRKANCAVVLATQSISDA
ERSGIIDVLKESCPTKICLPNGAAREPGTREFYERIGFNERQIEIVATAIPKREYYVVSPEGRRLFNMAL
GPVALSFVGATGKEDLKQIRSLHSEHGAAWPLYWLQQRGIAHADTLISTS

  Protein domains


Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(186-400)

  Protein structure



No available structure.



ID   11219 GenBank   WP_209207926
Name   traG_J5289_RS27515_B230/85|unnamed2 insolico UniProt ID   _
Length   638 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 638 a.a.        Molecular weight: 68068.90 Da        Isoelectric Point: 9.8006

>WP_209207926.1 MULTISPECIES: Ti-type conjugative transfer system protein TraG [unclassified Rhizobium]
MTANRLLRGILPALIMLIAMALTTGMEHKLAALGTSPEAKLFLGRVGLALPYIAAAAVGVISLFAANGSP
NIKAAALSVLAGNGAVIVIAVVREAIRLAGIASSAPAGQSALAYTDPATMVGASAALFGGLFALRVAIKG
NAAFAQSGPTRIGGKRAVHGDASWMGMAEAGKLFPDTGGIVIGERYRVDRDSVAAMAFRTDDPHSWGDGG
KSPLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGGLVVLDPSSEVAPMVVEHRRKAGRKVIVLD
PGDRATGFNALDWIGRFGGTKEEDIVAVATWIMTDNARTASARDDFFRASAMQLLTALIADVCLSGNTDA
KDQTLRRVRANLSEPEPKLRERLTRIYEQSESTFVRENVAVFVNMTPETFSGVYANAVKETHWLSYTNYA
ALVSGDSFTTDELATGITDIFIALDLKVLESHPGLARVVIGSFLNAIYNRNGEVAGRTLFLLDEVARLGY
LRILETARDAGRKYGISLALIFQSIGQMRQSYGGRDASSKWFESASWISFAAINDPETAEYISKRCGDTT
VEVDQTNRSSGMKGSSRSRSKQLTRRPLILPHEVLRMRGDEQIVFTAGNAPLRCGRAIWFRRNDMSASVG
KTGFTGKP

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(164-632)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 85957..95619

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5289_RS27065 (J5289_27065) 81269..82480 - 1212 WP_209206924 plasmid replication protein RepC -
J5289_RS27070 (J5289_27070) 82649..83653 - 1005 WP_209206923 plasmid partitioning protein RepB -
J5289_RS27075 (J5289_27075) 83757..84965 - 1209 WP_209206918 plasmid partitioning protein RepA -
J5289_RS27080 (J5289_27080) 85322..85960 + 639 WP_209206916 acyl-homoserine-lactone synthase -
J5289_RS27085 (J5289_27085) 85957..86928 + 972 WP_209206914 P-type conjugative transfer ATPase TrbB virB11
J5289_RS27090 (J5289_27090) 86918..87322 + 405 WP_209206912 conjugal transfer pilin TrbC virB2
J5289_RS27095 (J5289_27095) 87315..87614 + 300 WP_209206910 conjugal transfer protein TrbD virB3
J5289_RS27100 (J5289_27100) 87624..90086 + 2463 WP_209206909 conjugal transfer protein TrbE virb4
J5289_RS27105 (J5289_27105) 90058..90864 + 807 WP_209206908 P-type conjugative transfer protein TrbJ virB5
J5289_RS27110 (J5289_27110) 90861..91076 + 216 WP_209206905 entry exclusion protein TrbK -
J5289_RS27115 (J5289_27115) 91070..92260 + 1191 WP_209206903 P-type conjugative transfer protein TrbL virB6
J5289_RS27120 (J5289_27120) 92278..92940 + 663 WP_209206901 conjugal transfer protein TrbF virB8
J5289_RS27125 (J5289_27125) 92988..93809 + 822 WP_247750685 P-type conjugative transfer protein TrbG virB9
J5289_RS27130 (J5289_27130) 93833..94285 + 453 WP_209206939 conjugal transfer protein TrbH -
J5289_RS27135 (J5289_27135) 94297..95619 + 1323 WP_209206890 IncP-type conjugal transfer protein TrbI virB10
J5289_RS27140 (J5289_27140) 96336..97394 + 1059 WP_209206888 HoxN/HupN/NixA family nickel/cobalt transporter -
J5289_RS27145 (J5289_27145) 97606..97899 - 294 WP_209206886 hypothetical protein -
J5289_RS27150 (J5289_27150) 98489..99202 - 714 WP_209206884 pentapeptide repeat-containing protein -
J5289_RS27155 (J5289_27155) 99311..99856 + 546 WP_209206882 TetR/AcrR family transcriptional regulator -

Host bacterium

ID   15458 GenBank   NZ_CP072159
Plasmid name   B230/85|unnamed2 Incompatibility group   -
Plasmid size   226706 bp Coordinate of oriT [Strand]   180109..180145 [+]
Host baterium   Rhizobium sp. B230/85

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -