Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 115000
• Name: oriT_LJBS17|unnamed2
• Organism: Bacillus sp. LJBS17
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP079761 (50473..50833 [+], 361 nt)
• oriT length: 361 nt
• IRs (inverted repeats): 291..296, 302..307 (AAAAAG..CTTTTT) 188..193, 198..203 (CACCAT..ATGGTG) 171..178, 180..187 (AAACCGCA..TGCGGTTT) 117..122, 130..135 (CATAAA..TTTATG) 103..110, 123..130 (AAAAAATG..CATTTTTT) 84..89, 92..97 (ATTTAG..CTAAAT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 361 nt

>oriT_LJBS17|unnamed2
AAAGAGCAATCTCGTCCTCGAAGATTAATTTTTAGTGTGATATACAGTTATTTTACGTGTGCATAAATTGAAGATTTAAGTGCATTTAGCTCTAAATCACCTAAAAAATGGTTGGTCATAAACATTTTTTTTATGTGCATTCAGCAATAAATCTGGTACCACGAAAAAACAAACCGCACTGCGGTTTCACCATGCAAATGGTGCCAGTTTCCCCTTATGCTCTTTCCTTTTCCACCCCCTCCTTCCTTCGGAATCGGGGGCCGGCTTTTTGCTGCCGCAAAAAGCCTTGGAAAAAGAAACACTTTTTTGGACAGATCGTTGCAAAGTATGTGCAGAAATCGATGAAAAGGAGCGTTAACAA

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobP2_KXZ66_RS22405_LJBS17|unnamed2

• ID: 9606
• GenBank: WP_072176350
• Name: mobP2_KXZ66_RS22405_LJBS17|unnamed2
• UniProt ID: _
• Length: 410 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 410 a.a.
• Molecular weight: 48472.99 Da
• Isoelectric Point: 10.0803

>WP_072176350.1 MULTISPECIES: MobP2 family relaxase [Bacillus]
MGSPGVVLVSKYVSGKSTKFSKYVNYINRDEAVRTEKFQTYNVNKLDGYNQYMGNPEKSSGIFTQHKDSL
SPKEKNQLKEIFRQAQKNDSVMWQDVISFDNKWLEERGIYNSKTGWVNEGAIQNSIRKGMEVLLREEQLE
QSGVWSAAIHYNTDNIHVHIALVEPNPTKEYGVFTNKKTGEVYQARRGNRKLKTLDKMKSKVANTLMDRD
KELSKISQLIHDRIAPKGLKFQPRLDTYMTKMYNHIYENLPEDMRLWKYNNNALNNIRPEIDSVITMYIQ
KYHPEDYKELDQSLKEEMEFRKSVYGDGPKQVERYKEYRKNKHKELYTKLGNSMLKEMAEIRRSESQSKQ
INRSGTYAPGSVSHRQWDTKGRRLKRSDINKIKHALDKDYQSMKNMRKYQQMQYEMEQSR

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (3-389)

Protein structure

No available structure.

T4CP

t4cp2_KXZ66_RS22355_LJBS17|unnamed2

• ID: 11206
• GenBank: WP_219144993
• Name: t4cp2_KXZ66_RS22355_LJBS17|unnamed2
• UniProt ID: _
• Length: 786 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 786 a.a.
• Molecular weight: 90635.18 Da
• Isoelectric Point: 4.9890

>WP_219144993.1 VirD4-like conjugal transfer protein, CD1115 family [Bacillus sp. LJBS17]
MHKEFKQWKKVFADKYFLIAFTLFCYVAVTCITNFFIHLLKQIPVILSSFKNFDQSQLSSPFSSMTWKWF
FEFDWSMGIVYGVVYVLASIFILRQVYRFRIAFRDINKQTKGTARWTEIKEIQETYKAVKDDDNEYEGSA
GMPIVHFNDHLYVDTNSTHTLVVASTQSGKTETYSYPYLDVIMRAKEKDSVVITDIKGDMLKNTRAEFEK
YGYEVMCFNLLNPYWGMAYNPLELVKQAYLKKDYSKAQMLCNTLSYSLFHNDKSHADPMWENASIALVNA
LILAVCDLCIKNGQPENITMYTLTVMLNELGSNPDEDGYTRLDHFFGNLPPSHPAKLQYGTIQFSQGITR
SGIYTGTMAKLKNYTYDTIARMTARNDLNIEDLAYGEKPVALFIVYPDWDDSNYTIISTFLSQVNAVLSE
KATLSKESTLPRKVRFLFEEVANIPPIEGLNRSLAVGLSRGMLYTLVIQNVSQLRDVYGDDMAVAIMGNL
GNQIYIMSDEWEDAEKFSEKLGVTTVISADRQGDLMDVHKSYSEREEERPLMLPDELRRLKKGEWVVLRT
KKREDLKRNRVVPYPIFASLDNGTNMLHRYEYLMHRFDNKIALGDLGFRGEHETLDLESLLIEFEFNEPV
KEERKSKPAKGKKQKAKKAPGEPVSSEFPLPEEPIGFTESSEGMDSLFASIPVENMNEHGFYEDPPMNES
LHEDDLEASEEDIIMNSLEEVYETSTPISDAIKPDHYVYIKLLAHKQLSESEYAYFDSLKTVEELRAFFR
APEKKEIYEQIKTHIE

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (145-567)

Protein structure

No available structure.

Host bacterium

• ID: 15435
• GenBank: NZ_CP079761
• Plasmid name: LJBS17|unnamed2
• Incompatibility group: -
• Plasmid size: 59827 bp
• Coordinate of oriT [Strand]: 50473..50833 [+]
• Host baterium: Bacillus sp. LJBS17

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -