Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 114999
• Name: oriT_pHD66_2
• Organism: Staphylococcus epidermidis strain HD66
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP040870 (9660..9757 [+], 98 nt)
• oriT length: 98 nt
• IRs (inverted repeats): _
• Location of nic site: 66..67
• Conserved sequence flanking the nic site: GATTGGTCGC
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 98 nt

>oriT_pHD66_2
TCAGCTGAAACTTTAAATATGAGTGTGCCTGCTTTCGTTAAGAAAAAGGCACAAGGCGCCCGATTGGTCGCACCCAAATTGGATCAAGCAACGCGACA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_FH488_RS12595_pHD66_2

• ID: 9605
• GenBank: WP_000246206
• Name: Relaxase_FH488_RS12595_pHD66_2
• UniProt ID: D2JGE5
• Length: 336 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 336 a.a.
• Molecular weight: 38461.30 Da
• Isoelectric Point: 7.3199

>WP_000246206.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Bacilli]
MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASRELYGKTDGNQGHVIIQSFKPGEVTPE
QCNQLGLALAEKLVPNHQVAVYTHNDTDHVHNHIVINSIDLETGKKFNNNKQALRNVRNFNDEVCMEHGL
SVPEKNTARLRYTQTEKAIADPNTKSTAQYSWKDEIREAIDQSQATNMDEFKDHLNQHGIEIERVTPKSI
TYRHLAEDKKVRGRKLGEDYNKGGIEDGFERQIQHQQQTERVSDTDVQPKRRTSSRDESTERDTGVTQAD
WDQFAQDTNELERSRQAAESARLADEKARRDREKRERAKQTSRRIINNDRDHGLEL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-242)

Protein structure

• AlphaFold DB: D2JGE5

Host bacterium

• ID: 15434
• GenBank: NZ_CP040870
• Plasmid name: pHD66_2
• Incompatibility group: -
• Plasmid size: 13701 bp
• Coordinate of oriT [Strand]: 9660..9757 [+]
• Host baterium: Staphylococcus epidermidis strain HD66

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -