Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 114992 |
| Name | oriT_pKM98_pOXA48  |
| Organism | Enterobacter hormaechei strain 21KM1498 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP126867 (40150..40255 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pKM98_pOXA48
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 9601 | GenBank | WP_004187323 |
| Name | traI_QPR64_RS24265_pKM98_pOXA48 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 11202 | GenBank | WP_004206885 |
| Name | t4cp2_QPR64_RS23970_pKM98_pOXA48 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 43051..62340
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QPR64_RS24245 (QPR64_24215) | 38135..38446 | + | 312 | WP_004187333 | hypothetical protein | - |
| QPR64_RS24250 (QPR64_24220) | 38579..39115 | + | 537 | WP_004187332 | hypothetical protein | - |
| QPR64_RS24255 (QPR64_24225) | 39617..40012 | - | 396 | WP_019725163 | hypothetical protein | - |
| QPR64_RS24260 (QPR64_24230) | 40047..40574 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| QPR64_RS24265 (QPR64_24235) | 40561..42540 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| QPR64_RS24270 (QPR64_24240) | 42554..43054 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| QPR64_RS24275 (QPR64_24245) | 43051..43830 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| QPR64_RS24280 (QPR64_24250) | 43841..45004 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| QPR64_RS24285 (QPR64_24255) | 44994..45254 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| QPR64_RS24290 (QPR64_24260) | 45279..48716 | + | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
| QPR64_RS24295 (QPR64_24265) | 48682..49194 | + | 513 | WP_011091071 | hypothetical protein | traL |
| QPR64_RS24300 (QPR64_24270) | 49195..49407 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| QPR64_RS24305 (QPR64_24275) | 49379..49789 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| QPR64_RS24310 (QPR64_24280) | 49857..50570 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| QPR64_RS24315 (QPR64_24285) | 50579..51730 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| QPR64_RS24320 (QPR64_24290) | 51742..53091 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| QPR64_RS24325 (QPR64_24295) | 53103..53807 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| QPR64_RS24330 (QPR64_24300) | 53831..54361 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| QPR64_RS24335 (QPR64_24305) | 54378..54767 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| QPR64_RS24340 (QPR64_24310) | 54813..55307 | + | 495 | WP_004187480 | hypothetical protein | - |
| QPR64_RS24345 (QPR64_24315) | 55304..58354 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| QPR64_RS24350 (QPR64_24320) | 58351..59559 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| QPR64_RS24355 (QPR64_24325) | 59556..60152 | + | 597 | WP_015060003 | hypothetical protein | - |
| QPR64_RS24360 (QPR64_24330) | 60145..62340 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| QPR64_RS24365 (QPR64_24335) | 62342..62995 | + | 654 | WP_015060005 | hypothetical protein | - |
| QPR64_RS24370 (QPR64_24340) | 63064..63306 | + | 243 | WP_023893611 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 15427 | GenBank | NZ_CP126867 |
| Plasmid name | pKM98_pOXA48 | Incompatibility group | IncL/M |
| Plasmid size | 63589 bp | Coordinate of oriT [Strand] | 40150..40255 [+] |
| Host baterium | Enterobacter hormaechei strain 21KM1498 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |