Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 114976 |
| Name | oriT_pEh15_OXA48  |
| Organism | Enterobacter hormaechei strain Ehh_15 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP126801 (39986..40091 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pEh15_OXA48
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 9592 | GenBank | WP_004187323 |
| Name | traI_QPR56_RS26905_pEh15_OXA48 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 11189 | GenBank | WP_004206885 |
| Name | t4cp2_QPR56_RS26610_pEh15_OXA48 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 42887..62176
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QPR56_RS26885 (QPR56_26885) | 37971..38282 | + | 312 | WP_004187333 | hypothetical protein | - |
| QPR56_RS26890 (QPR56_26890) | 38415..38951 | + | 537 | WP_004187332 | hypothetical protein | - |
| QPR56_RS26895 (QPR56_26895) | 39453..39848 | - | 396 | WP_019725163 | hypothetical protein | - |
| QPR56_RS26900 (QPR56_26900) | 39883..40410 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| QPR56_RS26905 (QPR56_26905) | 40397..42376 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| QPR56_RS26910 (QPR56_26910) | 42390..42890 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| QPR56_RS26915 (QPR56_26915) | 42887..43666 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| QPR56_RS26920 (QPR56_26920) | 43677..44840 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| QPR56_RS26925 (QPR56_26925) | 44830..45090 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| QPR56_RS26930 (QPR56_26930) | 45115..48552 | + | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
| QPR56_RS26935 (QPR56_26935) | 48518..49030 | + | 513 | WP_011091071 | hypothetical protein | traL |
| QPR56_RS26940 (QPR56_26940) | 49031..49243 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| QPR56_RS26945 (QPR56_26945) | 49215..49625 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| QPR56_RS26950 (QPR56_26950) | 49693..50406 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| QPR56_RS26955 (QPR56_26955) | 50415..51566 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| QPR56_RS26960 (QPR56_26960) | 51578..52927 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| QPR56_RS26965 (QPR56_26965) | 52939..53643 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| QPR56_RS26970 (QPR56_26970) | 53667..54197 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| QPR56_RS26975 (QPR56_26975) | 54214..54603 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| QPR56_RS26980 (QPR56_26980) | 54649..55143 | + | 495 | WP_004187480 | hypothetical protein | - |
| QPR56_RS26985 (QPR56_26985) | 55140..58190 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| QPR56_RS26990 (QPR56_26990) | 58187..59395 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| QPR56_RS26995 (QPR56_26995) | 59392..59988 | + | 597 | WP_015060003 | hypothetical protein | - |
| QPR56_RS27000 (QPR56_27000) | 59981..62176 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| QPR56_RS27005 (QPR56_27005) | 62178..62831 | + | 654 | WP_015060005 | hypothetical protein | - |
| QPR56_RS27010 (QPR56_27010) | 62912..63142 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 15411 | GenBank | NZ_CP126801 |
| Plasmid name | pEh15_OXA48 | Incompatibility group | IncL/M |
| Plasmid size | 63437 bp | Coordinate of oriT [Strand] | 39986..40091 [+] |
| Host baterium | Enterobacter hormaechei strain Ehh_15 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |