Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114959
Name   oriT_Carbapenemase(IMP-26)_IncN in_silico
Organism   Enterobacter cloacae
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP050159 (43611..43711 [+], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      80..85, 91..96  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_Carbapenemase(IMP-26)_IncN
TATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9582 GenBank   WP_012561166
Name   mobF_HBA71_RS00250_Carbapenemase(IMP-26)_IncN insolico UniProt ID   D7RU03
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120141.30 Da        Isoelectric Point: 6.5229

>WP_012561166.1 MULTISPECIES: MobF family relaxase [Gammaproteobacteria]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKNEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure


Source ID Structure
AlphaFold DB D7RU03


Auxiliary protein

ID   5626 GenBank   WP_001749975
Name   WP_001749975_Carbapenemase(IMP-26)_IncN insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   11172 GenBank   WP_000342688
Name   t4cp2_HBA71_RS00255_Carbapenemase(IMP-26)_IncN insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 16140..26465

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HBA71_RS00065 11985..13418 - 1434 WP_001288432 DNA cytosine methyltransferase -
HBA71_RS00070 13517..13789 + 273 Protein_14 IS1 family transposase -
HBA71_RS00075 13800..14006 - 207 WP_001749967 hypothetical protein -
HBA71_RS00080 14011..14658 - 648 WP_015344958 restriction endonuclease -
HBA71_RS00085 14708..14941 - 234 WP_001191790 hypothetical protein -
HBA71_RS00090 15055..15369 - 315 WP_001749965 TrbM/KikA/MpfK family conjugal transfer protein -
HBA71_RS00095 15366..15710 - 345 WP_001749964 hypothetical protein -
HBA71_RS00100 15726..16097 - 372 WP_011867773 H-NS family nucleoid-associated regulatory protein -
HBA71_RS00105 16140..16874 + 735 WP_001749963 lytic transglycosylase domain-containing protein virB1
HBA71_RS00110 16883..17164 + 282 WP_000440698 transcriptional repressor KorA -
HBA71_RS00115 17174..17467 + 294 WP_001749962 hypothetical protein virB2
HBA71_RS00120 17517..17834 + 318 WP_000496058 VirB3 family type IV secretion system protein virB3
HBA71_RS00125 17834..20434 + 2601 WP_001749961 VirB4 family type IV secretion/conjugal transfer ATPase virb4
HBA71_RS00130 20452..21165 + 714 WP_001749960 type IV secretion system protein virB5
HBA71_RS00135 21173..21400 + 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
HBA71_RS00140 21416..22456 + 1041 WP_001749958 type IV secretion system protein virB6
HBA71_RS00145 22527..22685 + 159 WP_012561180 hypothetical protein -
HBA71_RS00150 22675..23373 + 699 WP_000646594 virB8 family protein virB8
HBA71_RS00155 23384..24268 + 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
HBA71_RS00160 24268..25428 + 1161 WP_000101710 type IV secretion system protein VirB10 virB10
HBA71_RS00165 25470..26465 + 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
HBA71_RS00170 26465..26998 + 534 WP_000792636 phospholipase D family protein -
HBA71_RS00370 27172..27477 - 306 WP_011742557 DUF6710 family protein -
HBA71_RS00185 27726..27905 + 180 Protein_36 helix-turn-helix domain-containing protein -
HBA71_RS00190 27970..28674 + 705 WP_001067855 IS6-like element IS26 family transposase -
HBA71_RS00195 28728..29359 - 632 Protein_38 transposase -
HBA71_RS00200 29754..30410 + 657 WP_001516695 quinolone resistance pentapeptide repeat protein QnrS1 -
HBA71_RS00205 30729..31343 - 615 WP_014839983 recombinase family protein -

Host bacterium

ID   15394 GenBank   NZ_CP050159
Plasmid name   Carbapenemase(IMP-26)_IncN Incompatibility group   IncN
Plasmid size   56780 bp Coordinate of oriT [Strand]   43611..43711 [+]
Host baterium   Enterobacter cloacae

Cargo genes

Drug resistance gene   blaIMP-26, qnrS1, blaTEM-1B
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -