Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 114856 |
| Name | oriT_pL22-5  |
| Organism | Klebsiella quasipneumoniae strain L22 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP031262 (21419..21467 [-], 49 nt) |
| oriT length | 49 nt |
| IRs (inverted repeats) | 6..13, 16..23 (GCAAAATT..AATTTTGC) |
| Location of nic site | 32..33 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 49 nt
>oriT_pL22-5
AATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 9524 | GenBank | WP_013023833 |
| Name | traI_DV026_RS28330_pL22-5 |
UniProt ID | D4HQM5 |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190722.84 Da Isoelectric Point: 6.3385
>WP_013023833.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Klebsiella]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKDAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDAFVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTGSSNMLTLKDREGVRLDLKVSAV
DSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQLRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLTQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKANQLITLTDSEGKERYISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDLERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKDAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDAFVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTGSSNMLTLKDREGVRLDLKVSAV
DSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQLRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLTQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKANQLITLTDSEGKERYISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDLERGYVANSIWEVQSVSGDSVTLSDGKLTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 11105 | GenBank | WP_013023832 |
| Name | traD_DV026_RS28325_pL22-5 |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 85974.10 Da Isoelectric Point: 5.1149
>WP_013023832.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAEEPSVRATEPSVLRLTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDYVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAEEPSVRATEPSVLRLTTVPLIKPKAAAAAAAAATASSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDYVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 20861..50747
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| DV026_RS28090 | 16868..17224 | + | 357 | WP_013023814 | hypothetical protein | - |
| DV026_RS28095 | 17285..17497 | + | 213 | WP_013023815 | hypothetical protein | - |
| DV026_RS28100 | 17508..17732 | + | 225 | WP_004152719 | hypothetical protein | - |
| DV026_RS28105 | 17813..18133 | + | 321 | WP_004152720 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| DV026_RS28110 | 18123..18401 | + | 279 | WP_004152721 | helix-turn-helix transcriptional regulator | - |
| DV026_RS28115 | 18402..18815 | + | 414 | WP_013023817 | type II toxin-antitoxin system HigA family antitoxin | - |
| DV026_RS28135 | 19645..20466 | + | 822 | WP_004152492 | DUF932 domain-containing protein | - |
| DV026_RS28140 | 20499..20828 | + | 330 | WP_011977736 | DUF5983 family protein | - |
| DV026_RS28145 | 20861..21346 | - | 486 | WP_001568108 | transglycosylase SLT domain-containing protein | virB1 |
| DV026_RS28150 | 21778..22170 | + | 393 | WP_004194114 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| DV026_RS28155 | 22400..23101 | + | 702 | WP_004194113 | hypothetical protein | - |
| DV026_RS28160 | 23187..23387 | + | 201 | WP_004194116 | TraY domain-containing protein | - |
| DV026_RS28165 | 23456..23824 | + | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
| DV026_RS28170 | 23838..24143 | + | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| DV026_RS28175 | 24163..24729 | + | 567 | WP_004144423 | type IV conjugative transfer system protein TraE | traE |
| DV026_RS28180 | 24716..25456 | + | 741 | WP_013023821 | type-F conjugative transfer system secretin TraK | traK |
| DV026_RS28185 | 25456..26880 | + | 1425 | WP_004194260 | F-type conjugal transfer pilus assembly protein TraB | traB |
| DV026_RS28190 | 26994..27578 | + | 585 | WP_013023822 | type IV conjugative transfer system lipoprotein TraV | traV |
| DV026_RS28195 | 27710..28120 | + | 411 | WP_009309869 | hypothetical protein | - |
| DV026_RS28200 | 28226..28444 | + | 219 | WP_015344987 | hypothetical protein | - |
| DV026_RS28205 | 28445..28756 | + | 312 | WP_015344986 | hypothetical protein | - |
| DV026_RS28210 | 28823..29227 | + | 405 | WP_004197817 | hypothetical protein | - |
| DV026_RS28220 | 29491..30459 | - | 969 | WP_077256550 | IS5-like element IS903B family transposase | - |
| DV026_RS28225 | 30669..31067 | + | 399 | WP_013609531 | hypothetical protein | - |
| DV026_RS28230 | 31139..33778 | + | 2640 | WP_013023824 | type IV secretion system protein TraC | virb4 |
| DV026_RS28235 | 33778..34167 | + | 390 | WP_004197815 | type-F conjugative transfer system protein TrbI | - |
| DV026_RS28240 | 34167..34793 | + | 627 | WP_009309871 | type-F conjugative transfer system protein TraW | traW |
| DV026_RS28245 | 34835..35224 | + | 390 | WP_004194992 | hypothetical protein | - |
| DV026_RS28250 | 35221..36210 | + | 990 | WP_009309872 | conjugal transfer pilus assembly protein TraU | traU |
| DV026_RS28255 | 36223..36861 | + | 639 | WP_011977786 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| DV026_RS28260 | 36920..38875 | + | 1956 | WP_013023827 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| DV026_RS28265 | 38907..39161 | + | 255 | WP_004152674 | conjugal transfer protein TrbE | - |
| DV026_RS28270 | 39139..39387 | + | 249 | WP_004152675 | hypothetical protein | - |
| DV026_RS28275 | 39400..39726 | + | 327 | WP_004152676 | hypothetical protein | - |
| DV026_RS28280 | 39747..40499 | + | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| DV026_RS28285 | 40510..40749 | + | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| DV026_RS28290 | 40721..41278 | + | 558 | WP_013214031 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| DV026_RS28295 | 41324..41767 | + | 444 | WP_013023828 | F-type conjugal transfer protein TrbF | - |
| DV026_RS28300 | 41745..43124 | + | 1380 | WP_014343487 | conjugal transfer pilus assembly protein TraH | traH |
| DV026_RS28305 | 43124..45973 | + | 2850 | WP_013609534 | conjugal transfer mating-pair stabilization protein TraG | traG |
| DV026_RS28310 | 45976..46509 | + | 534 | WP_014343486 | conjugal transfer protein TraS | - |
| DV026_RS28315 | 46695..47426 | + | 732 | WP_013023830 | conjugal transfer complement resistance protein TraT | - |
| DV026_RS29450 | 47619..48308 | + | 690 | WP_013023831 | hypothetical protein | - |
| DV026_RS28325 | 48435..50747 | + | 2313 | WP_013023832 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
| ID | 15291 | GenBank | NZ_CP031262 |
| Plasmid name | pL22-5 | Incompatibility group | IncFII |
| Plasmid size | 95314 bp | Coordinate of oriT [Strand] | 21419..21467 [-] |
| Host baterium | Klebsiella quasipneumoniae strain L22 |
Cargo genes
| Drug resistance gene | blaCTX-M-15, blaTEM-1B |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |