Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 114855 |
| Name | oriT_pL22-4  |
| Organism | Klebsiella quasipneumoniae strain L22 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP031261 (47807..47888 [-], 82 nt) |
| oriT length | 82 nt |
| IRs (inverted repeats) | 27..32, 35..40 (GCCACC..GGTGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 82 nt
>oriT_pL22-4
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 9523 | GenBank | WP_032440569 |
| Name | traI_DV026_RS27760_pL22-4 |
UniProt ID | A0A7G3L4G1 |
| Length | 642 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 642 a.a. Molecular weight: 74384.24 Da Isoelectric Point: 8.3203
>WP_032440569.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7G3L4G1 |
T4CP
| ID | 11104 | GenBank | WP_032440593 |
| Name | trbC_DV026_RS27625_pL22-4 |
UniProt ID | _ |
| Length | 730 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 730 a.a. Molecular weight: 82679.78 Da Isoelectric Point: 6.3201
>WP_032440593.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 16747..38698
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| DV026_RS27615 | 12510..13205 | - | 696 | WP_254910806 | RepA family replication protein | - |
| DV026_RS29375 | 13711..13962 | - | 252 | WP_113394424 | hypothetical protein | - |
| DV026_RS27620 | 13974..14507 | - | 534 | WP_032440594 | phospholipase D family protein | - |
| DV026_RS27625 | 14553..16745 | - | 2193 | WP_032440593 | F-type conjugative transfer protein TrbC | - |
| DV026_RS27630 | 16747..17901 | - | 1155 | WP_157769471 | thioredoxin fold domain-containing protein | trbB |
| DV026_RS27635 | 17849..19267 | - | 1419 | WP_063666705 | hypothetical protein | trbA |
| DV026_RS27640 | 19369..19992 | - | 624 | WP_032440592 | plasmid IncI1-type surface exclusion protein ExcA | - |
| DV026_RS27645 | 20058..22202 | - | 2145 | WP_032440591 | DotA/TraY family protein | traY |
| DV026_RS27650 | 22244..22825 | - | 582 | WP_050597335 | conjugal transfer protein TraX | - |
| DV026_RS27655 | 22822..24018 | - | 1197 | WP_032440590 | conjugal transfer protein TraW | traW |
| DV026_RS27660 | 24028..27075 | - | 3048 | WP_032440589 | conjugal transfer protein | traU |
| DV026_RS29380 | 27085..27648 | - | 564 | WP_045325070 | hypothetical protein | traT |
| DV026_RS27670 | 27692..28093 | - | 402 | WP_032440588 | DUF6750 family protein | traR |
| DV026_RS27675 | 28149..28682 | - | 534 | WP_032440587 | conjugal transfer protein TraQ | traQ |
| DV026_RS27680 | 28692..29411 | - | 720 | WP_050597333 | conjugal transfer protein TraP | traP |
| DV026_RS27685 | 29418..30758 | - | 1341 | WP_032440586 | conjugal transfer protein TraO | traO |
| DV026_RS27690 | 30762..31865 | - | 1104 | WP_077264204 | DotH/IcmK family type IV secretion protein | traN |
| DV026_RS27695 | 31876..32544 | - | 669 | WP_032440585 | DotI/IcmL/TraM family protein | traM |
| DV026_RS27700 | 32616..32969 | - | 354 | WP_032440584 | hypothetical protein | traL |
| DV026_RS27705 | 33003..36434 | - | 3432 | WP_117985405 | LPD7 domain-containing protein | - |
| DV026_RS27710 | 36452..36718 | - | 267 | WP_032440577 | IcmT/TraK family protein | traK |
| DV026_RS27715 | 36715..37902 | - | 1188 | WP_077255878 | plasmid transfer ATPase TraJ | virB11 |
| DV026_RS27720 | 37880..38698 | - | 819 | WP_032440576 | type IV secretory system conjugative DNA transfer family protein | traI |
| DV026_RS27725 | 38695..39159 | - | 465 | WP_225546581 | DotD/TraH family lipoprotein | - |
| DV026_RS27730 | 39185..39664 | - | 480 | WP_032440574 | lytic transglycosylase domain-containing protein | - |
| DV026_RS27735 | 40396..40623 | - | 228 | WP_032440573 | hypothetical protein | - |
| DV026_RS27740 | 41233..42075 | + | 843 | WP_032440572 | helix-turn-helix domain-containing protein | - |
| DV026_RS27745 | 42135..43064 | + | 930 | WP_032440581 | glycosyltransferase family 9 protein | - |
| DV026_RS27750 | 43057..43551 | + | 495 | WP_032440571 | molybdopterin-dependent oxidoreductase | - |
Host bacterium
| ID | 15290 | GenBank | NZ_CP031261 |
| Plasmid name | pL22-4 | Incompatibility group | IncFII |
| Plasmid size | 66154 bp | Coordinate of oriT [Strand] | 47807..47888 [-] |
| Host baterium | Klebsiella quasipneumoniae strain L22 |
Cargo genes
| Drug resistance gene | qnrS1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |