Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114786
Name   oriT_1382|P1 in_silico
Organism   Enterobacter cloacae isolate 1382
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OW968329 (33310..33661 [+], 352 nt)
oriT length   352 nt
IRs (inverted repeats)      255..262, 271..278  (ACCGCTAG..CTAGCGGT)
 192..198, 206..212  (TATAAAA..TTTTATA)
 40..47, 50..57  (GCAAAAAC..GTTTTTGC)
 4..11, 16..23  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 352 nt

>oriT_1382|P1
AGGTTGGTGGTTCTCACCACCAAAAGCACCACACACTACGCAAAAACAAGTTTTTGCTGATTTTTCTTTATAAATAGAGTGTTATGAAAAATTAGTTTCTCTTACTCTCTTTATGATATTTAAAAAAGCGGTGTCGGCGCGGCTACAACAACGCGCCGACACCGCTTTGTAGGGGTGTTACTGACTATTTTTATAAAAAACATCATTTTATATTAGGGGCGCTGCTAGCGGCGCGGTGTGTTTTTTTATAGGATACCGCTAGGGGCGCTGCTAGCGGTGCGTCCCTGTTTGTATTATGAATTTTAGTGTTCCGAAATTAACTTTATTTTATGTTCAAAAAAGGTAATCTCTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9474 GenBank   WP_230137320
Name   traI_LQ108_RS23610_1382|P1 insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191757.41 Da        Isoelectric Point: 5.8509

>WP_230137320.1 conjugative transfer relaxase/helicase TraI [Enterobacter cloacae]
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMLKGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
EPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLSGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQEGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGRTEQAVREIAGQERDRAAITEREAALPESVLRESQREREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

DNA helicase TraI (TraI_C)

(1434-1557)

TrwC relaxase (TrwC)

(10-283)

AAA domain (AAA_30)

(968-1156)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(575-626)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(633-712)


  Protein structure



No available structure.




Auxiliary protein

ID   5555 GenBank   WP_001151538
Name   WP_001151538_1382|P1 insolico UniProt ID   A0A8B3KK21
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14559.51 Da        Isoelectric Point: 4.8662

>WP_001151538.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacteriaceae]
MAKVNLYISNDAYEKINSIIEKRRQEGAREKDVSFSATASMLLELGLRVYEAQMERKESAFNQTEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSSEMERFFPKNDDE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-126)


  Protein structure


Source ID Structure
AlphaFold DB A0A8B3KK21

ID   5556 GenBank   WP_230137317
Name   WP_230137317_1382|P1 insolico UniProt ID   _
Length   133 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 133 a.a.        Molecular weight: 15410.69 Da        Isoelectric Point: 9.7066

>WP_230137317.1 MULTISPECIES: conjugal transfer relaxosome DNA-bindin protein TraY [Enterobacteriaceae]
MLLKRFGTRSATGKMVKLKLPVDVESLLIEASNRSGRSRSFEAVIRLKDHLHRYPKFNRAGNIYGKSLVK
YLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVIDHLIKFPDFYNSEIFREADKEEDITFDTL

  Protein domains


Predicted by InterproScan.

TraY domain (TraY)

(18-61)

TraY domain (TraY)

(72-119)


  Protein structure



No available structure.




T4CP

ID   11022 GenBank   WP_021526637
Name   traC_LQ108_RS23505_1382|P1 insolico UniProt ID   _
Length   876 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 876 a.a.        Molecular weight: 99210.94 Da        Isoelectric Point: 6.2300

>WP_021526637.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacteriaceae]
MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAI
PINGANKSIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAA
ATQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIV
GEMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNPEIAF
LWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWG
ELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGK
GLFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSG
AGKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLS
VMASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMIVLLDQY
TANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGW
RLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKY
NQLFPDQFQPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEG
LSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

TraC protein (TraC_F_IV)

(39-277)

TraG P-loop domain (P-loop_TraG)

(468-768)

Family of unknown function (DUF5934) (DUF5934)

(290-447)

  Protein structure



No available structure.



ID   11023 GenBank   WP_128867106
Name   traD_LQ108_RS23605_1382|P1 insolico UniProt ID   _
Length   747 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 747 a.a.        Molecular weight: 85078.92 Da        Isoelectric Point: 5.2345

>WP_128867106.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLVLWVKISWQTFINGCIYWWCTSLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYAR
KRGDMVVIYDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVVSMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIA
EFAAGEIGEKEHLKASLQYSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQTRPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQAEQPQQPQQPQQPQQ
PQQPQQPQQPQQPQQPQQPQQSQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESG
EVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(173-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 32739..62509

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ108_RS23380 (AI2999V1_4532) 28035..28349 + 315 WP_000872086 hypothetical protein -
LQ108_RS23385 28499..28651 + 153 Protein_35 DUF5431 family protein -
LQ108_RS23390 (AI2999V1_4533) 28596..28718 + 123 WP_223200913 Hok/Gef family protein -
LQ108_RS23395 29019..29268 - 250 Protein_37 hypothetical protein -
LQ108_RS23400 (AI2999V1_4534) 29564..29887 + 324 WP_000533253 hypothetical protein -
LQ108_RS23405 (AI2999V1_4535) 29946..30188 + 243 WP_000540588 hypothetical protein -
LQ108_RS23410 30454..30744 + 291 WP_073972341 hypothetical protein -
LQ108_RS23415 (AI2999V1_4536) 31215..31511 + 297 WP_001272251 hypothetical protein -
LQ108_RS23420 (AI2999V1_4537) 31621..32442 + 822 WP_001234445 DUF932 domain-containing protein -
LQ108_RS23425 (AI2999V1_4538) 32739..33341 - 603 WP_077761845 transglycosylase SLT domain-containing protein virB1
LQ108_RS23430 (AI2999V1_4539) 33662..34045 + 384 WP_001151538 conjugal transfer relaxosome DNA-binding protein TraM -
LQ108_RS23435 (AI2999V1_4540) 34232..34921 + 690 WP_021542442 conjugal transfer transcriptional regulator TraJ -
LQ108_RS23440 35020..35415 + 396 WP_348637393 conjugal transfer relaxosome DNA-bindin protein TraY -
LQ108_RS23445 (AI2999V1_4541) 35448..35810 + 363 WP_021542441 type IV conjugative transfer system pilin TraA -
LQ108_RS23450 (AI2999V1_4542) 35825..36136 + 312 WP_000012100 type IV conjugative transfer system protein TraL traL
LQ108_RS23455 (AI2999V1_4543) 36158..36724 + 567 WP_021503373 type IV conjugative transfer system protein TraE traE
LQ108_RS23460 (AI2999V1_4544) 36711..37439 + 729 WP_001230769 type-F conjugative transfer system secretin TraK traK
LQ108_RS23465 (AI2999V1_4545) 37439..38866 + 1428 WP_115763536 F-type conjugal transfer pilus assembly protein TraB traB
LQ108_RS23470 (AI2999V1_4546) 38856..39446 + 591 WP_021542439 conjugal transfer pilus-stabilizing protein TraP -
LQ108_RS23475 (AI2999V1_4547) 39433..39753 + 321 WP_024188751 conjugal transfer protein TrbD -
LQ108_RS23480 (AI2999V1_4548) 39750..40265 + 516 WP_001681800 type IV conjugative transfer system lipoprotein TraV traV
LQ108_RS23485 40400..40621 + 222 WP_001278689 conjugal transfer protein TraR -
LQ108_RS23490 (AI2999V1_4549) 40614..41087 + 474 WP_021542437 hypothetical protein -
LQ108_RS23495 (AI2999V1_4550) 41167..41382 + 216 WP_021542436 hypothetical protein -
LQ108_RS23500 41410..41772 + 363 WP_021542435 hypothetical protein -
LQ108_RS23505 (AI2999V1_4551) 41898..44528 + 2631 WP_021526637 type IV secretion system protein TraC virb4
LQ108_RS23510 (AI2999V1_4552) 44525..44911 + 387 WP_000214084 type-F conjugative transfer system protein TrbI -
LQ108_RS23515 (AI2999V1_4553) 44908..45540 + 633 WP_021526638 type-F conjugative transfer system protein TraW traW
LQ108_RS23520 (AI2999V1_4554) 45537..46529 + 993 WP_000830838 conjugal transfer pilus assembly protein TraU traU
LQ108_RS23525 (AI2999V1_4555) 46559..46864 + 306 WP_000224418 hypothetical protein -
LQ108_RS23530 (AI2999V1_4556) 46873..47511 + 639 WP_001080251 type-F conjugative transfer system pilin assembly protein TrbC trbC
LQ108_RS23535 (AI2999V1_4557) 47508..49358 + 1851 WP_050009854 type-F conjugative transfer system mating-pair stabilization protein TraN traN
LQ108_RS23540 (AI2999V1_4558) 49385..49642 + 258 WP_000864314 conjugal transfer protein TrbE -
LQ108_RS23545 (AI2999V1_4559) 49635..50378 + 744 WP_001030385 type-F conjugative transfer system pilin assembly protein TraF traF
LQ108_RS23550 (AI2999V1_4560) 50392..50745 + 354 WP_089564714 conjugal transfer protein TrbA -
LQ108_RS23555 (AI2999V1_4562) 51177..52322 - 1146 WP_089564715 hypothetical protein -
LQ108_RS23560 (AI2999V1_4563) 52379..52660 + 282 WP_089564716 type-F conjugative transfer system pilin chaperone TraQ -
LQ108_RS23565 (AI2999V1_4564) 52647..53192 + 546 WP_000059830 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
LQ108_RS23570 (AI2999V1_4565) 53122..53469 + 348 WP_136747986 P-type conjugative transfer protein TrbJ -
LQ108_RS23575 (AI2999V1_4566) 53450..53842 + 393 WP_014106973 F-type conjugal transfer protein TrbF -
LQ108_RS23580 (AI2999V1_4567) 53829..55202 + 1374 WP_014106974 conjugal transfer pilus assembly protein TraH traH
LQ108_RS23585 (AI2999V1_4568) 55199..58024 + 2826 WP_113328789 conjugal transfer mating-pair stabilization protein TraG traG
LQ108_RS23590 (AI2999V1_4569) 58021..58530 + 510 WP_000628111 conjugal transfer entry exclusion protein TraS -
LQ108_RS23595 (AI2999V1_4570) 58544..59275 + 732 WP_012881123 conjugal transfer complement resistance protein TraT -
LQ108_RS23600 (AI2999V1_4571) 59478..60215 + 738 WP_012881122 hypothetical protein -
LQ108_RS23605 (AI2999V1_4572) 60266..62509 + 2244 WP_128867106 type IV conjugative transfer system coupling protein TraD prgC

Host bacterium

ID   15221 GenBank   NZ_OW968329
Plasmid name   1382|P1 Incompatibility group   -
Plasmid size   72965 bp Coordinate of oriT [Strand]   33310..33661 [+]
Host baterium   Enterobacter cloacae isolate 1382

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -