Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114784
Name   oriT_Ah-HSP|unnamed in_silico
Organism   Aeromonas hydrophila strain Ah-HSP
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CM007660 (39449..39599 [-], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_Ah-HSP|unnamed
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9472 GenBank   WP_001337757
Name   mobH_BWQ95_RS00225_Ah-HSP|unnamed insolico UniProt ID   A0A709FRI2
Length   992 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A709FRI2


T4CP

ID   11019 GenBank   WP_000637386
Name   traC_BWQ95_RS00145_Ah-HSP|unnamed insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.



ID   11020 GenBank   WP_000178856
Name   traD_BWQ95_RS00220_Ah-HSP|unnamed insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 16324..41515

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWQ95_RS00085 (BWQ95_07245) 11451..12359 - 909 WP_000739139 hypothetical protein -
BWQ95_RS00090 (BWQ95_07250) 12370..13338 - 969 WP_000085162 AAA family ATPase -
BWQ95_RS00095 (BWQ95_07255) 13558..13743 - 186 WP_001186917 hypothetical protein -
BWQ95_RS00100 (BWQ95_07260) 14047..14367 - 321 WP_000547566 hypothetical protein -
BWQ95_RS00105 (BWQ95_07265) 14661..15302 + 642 WP_000796664 hypothetical protein -
BWQ95_RS00110 (BWQ95_07270) 15425..16285 + 861 WP_000709517 hypothetical protein -
BWQ95_RS00115 (BWQ95_07275) 16324..19122 - 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
BWQ95_RS00120 (BWQ95_07280) 19238..20242 - 1005 WP_227511115 TraU family protein traU
BWQ95_RS00125 (BWQ95_07285) 20242..20913 - 672 WP_001337754 EAL domain-containing protein -
BWQ95_RS00130 (BWQ95_07290) 20910..22175 - 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
BWQ95_RS00135 (BWQ95_07295) 22138..22668 - 531 WP_001010738 S26 family signal peptidase -
BWQ95_RS00140 (BWQ95_07300) 22665..22982 - 318 WP_000351984 hypothetical protein -
BWQ95_RS00145 (BWQ95_07305) 22997..25444 - 2448 WP_000637386 type IV secretion system protein TraC virb4
BWQ95_RS00150 (BWQ95_07310) 25441..26148 - 708 WP_001259347 DsbC family protein -
BWQ95_RS00155 (BWQ95_07315) 26297..31828 - 5532 WP_000606833 Ig-like domain-containing protein -
BWQ95_RS00160 (BWQ95_07320) 32029..32421 - 393 WP_000479535 TraA family conjugative transfer protein -
BWQ95_RS00165 (BWQ95_07325) 32425..33003 - 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
BWQ95_RS00170 (BWQ95_07330) 33000..34313 - 1314 WP_024131605 TraB/VirB10 family protein traB
BWQ95_RS00175 (BWQ95_07335) 34313..35230 - 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
BWQ95_RS00180 (BWQ95_07340) 35214..35840 - 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
BWQ95_RS00185 (BWQ95_07345) 35837..36118 - 282 WP_000805625 type IV conjugative transfer system protein TraL traL
BWQ95_RS00190 (BWQ95_07350) 36263..36628 - 366 WP_001052531 hypothetical protein -
BWQ95_RS00195 (BWQ95_07355) 36964..37626 - 663 WP_001231463 hypothetical protein -
BWQ95_RS00200 (BWQ95_07360) 37626..38003 - 378 WP_000869261 hypothetical protein -
BWQ95_RS00205 (BWQ95_07365) 38013..38459 - 447 WP_000122506 hypothetical protein -
BWQ95_RS00210 (BWQ95_07370) 38469..39098 - 630 WP_000743450 DUF4400 domain-containing protein tfc7
BWQ95_RS00215 (BWQ95_07375) 39055..39600 - 546 WP_000228720 hypothetical protein -
BWQ95_RS00220 (BWQ95_07380) 39650..41515 - 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
BWQ95_RS00225 (BWQ95_07385) 41512..44490 - 2979 WP_001337757 MobH family relaxase -
BWQ95_RS00230 (BWQ95_07390) 44658..45275 + 618 WP_001249396 hypothetical protein -
BWQ95_RS00235 (BWQ95_07395) 45257..45490 + 234 WP_001191892 hypothetical protein -

Host bacterium

ID   15219 GenBank   NZ_CM007660
Plasmid name   Ah-HSP|unnamed Incompatibility group   IncA/C2
Plasmid size   119875 bp Coordinate of oriT [Strand]   39449..39599 [-]
Host baterium   Aeromonas hydrophila strain Ah-HSP

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   merR, merT, merP, merC, merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -