Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114758
Name   oriT_1760953|unnamed2 in_silico
Organism   Shinella sumterensis strain 1760953
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP132304 (153630..153658 [+], 29 nt)
oriT length   29 nt
IRs (inverted repeats)      14..19, 24..29  (CGTCGC..GCGACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 29 nt

>oriT_1760953|unnamed2
AGGGCGCAATATACGTCGCTAGCGCGACG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9459 GenBank   WP_306040480
Name   traA_Q9313_RS25110_1760953|unnamed2 insolico UniProt ID   _
Length   1538 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1538 a.a.        Molecular weight: 169450.22 Da        Isoelectric Point: 9.5629

>WP_306040480.1 Ti-type conjugative transfer relaxase TraA [Shinella sumterensis]
MAIMFVRAQVISRGAGRSIVSAAAYRHRARMMDEQVGTSFSYRGGAAELKHEELALPDQVPAWLRTAIEG
KSVAAASEALWNAVEAFETRADAQLARELIIALPEELTRAENIALVQEFVRDNLTSKGMIADWVYHDKDG
NPHIHLMTALRPLMEEGFGPKKVPVLGEDGEPLRVVTPDRPKGKIVYKLWAGDKETMKAWKIGWAETANR
HLALAGHDIRLDGRSYAEQGLDGIAQKHLGPEKAALARKGVEMYFAPADLARRQDVADRLLADPELLLKQ
LGNERSTFDEKDIAKALHRTVDDPTDFANIRARLMASDDLVLLKPQRADRHTGKVAESAVFTTRGILRIE
YDMAQSAEALSKRTGFGVADTKVAAAVRQVETADPVKPFKLDQEQVDAIRHVTGDNGIAAVVGLAGAGKS
TLLAAARVAWEGEGHRIFGAALAGKAAEGLEDSSGIKSRTLASWELSWENGRDLLDRGDIFVVDEAGMVS
SQQMARVLKAAEDAGAKVVLVGDAMQLQPIQAGAAFRAISERIGSAELAGVRRQREAWARDASRLFARGK
VEEGLDAYAQRGRIVETEQRVEVVERIVADWSEARNGAIARSQAAGNEGKLRGDEMLVLAHTNEDVKRLN
ESLRGVMNAAGALTEAREFQTERGVREFAAGDRIIFLENARFLEPRARRLGPQYVKNGMLGTVVATGDVR
GGPLLSVRLDNGRDVVISEDSYRNVDHGYAATIHKSQGATVDRTFILATGMMDQHLTYVSMTRHRDQATL
YAAREDFEAKPEWGRKPRADHAAGVTGELVETGKAKFRPEDKDAKESPYADVKTDDGVVHRVWGVSLPKA
LAKGGVSEGDTVTLRKDGVETVKVDVVVTGPETGEKRTEKREVQRNVWTAKLKESADARQERIARESHRP
ELFRQMVERLGRSGAKTTTLDFEAEAGYQMHARDFARRRGIDLAAEVAAGMEEGAARRLAWLAEKRGQVA
KLWERASVALGFAIERERSVSYNEERSEHLAAEIPTDRKHLVPPTTRFARSIEEDARRAQLGSDRWKERE
AILRPVLEKIYRDPDGALARLNVLTSNSRIEPRQLAADLAATPQRLGRLHGSEHLVDGRIARDERNAAMA
ALLDLLPLARAHATEFRRQAERFGIREEQRRAHMSLSIPALSKPAMARLVEIEAVRERGGPDAYKTAFAY
AVEDRLLVQEVKAVNDALTARFGWSAFTEKADAIAARNMVERIPEDIAPDRREKLARLFTVVRRFAGEQH
LTERKDRSKMVAAASVEPGKETSAVLPMLAAVTEFKTSVEDEARVRAREVAHYGHNRAALVETAGRIWRD
PAGAVDKIEALVAKGFAGERIAAAVANDPAAYGALRGSDRIMDRLLAVGRERKDALQAVPEAESRVRSLG
ASYATALATETQAITEERQRMGVAIPGLSPAADEALRELAAAMKKDAKLNVAAGSLDARIWEEFAGVSRS
LDLRFGRDAILRGEKDVINRVSPAQRRPFEAMQERLRILQQTVRMGSSQRIVAERQRRAIDRGRGVIR

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(391-578)

MobA/MobL family (MobA_MobL)

(17-249)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(1310-1399)


  Protein structure



No available structure.




T4CP

ID   11003 GenBank   WP_306040471
Name   traG_Q9313_RS25095_1760953|unnamed2 insolico UniProt ID   _
Length   640 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 640 a.a.        Molecular weight: 70918.97 Da        Isoelectric Point: 8.9814

>WP_306040471.1 Ti-type conjugative transfer system protein TraG [Shinella sumterensis]
MMIRAKPHPSLLLVLVPIVVSGFAFYVANWRWPELATGMAGKTQYWFLRASPAPVLLFGPLAGLLTVWAL
PLHRRRPIAMVSFICFLLMTGFYGLREVARLSPYVGRGALSWDQALSFLDMVAVASAAAGFMAAAVSARI
SSVVPEPVKRAKTGTFGDADWLPMAAAARLFPEDGEIVVGERYRVDKELVHALPFDPNDPLTWGRGGNAP
LLTYKQDFDSTHMLFFAGSGGYKTTSNVVPTALRYTGPIICLDPSTEVAPMVVEHRRKTLRREVMVLDPT
NPIMGFNVLDGIETSTKKEEDIVGIAHMLLSESVRFESSTGSYFQNQAHNLLTGLLAHVMLSPEFADRRN
LRSLRQIVSEPETSVLAMLRDVQEHSASAFIRETLGVFVNMTEQTFSGVYSTASKDTQWLSLDNYAALVC
GNTFKSAEIAEGKKDVFLNIPASILRSYPGIGRVIIGSLINAMIEADGAFQRRALFMLDEVDLLGYMRVL
EEARDRGRKYGISMMLMYQSVGQLERHFGKDGAVSWIDGCAFASYAAIKALDTARNVSAQCGEMTVEVKG
SSRNIGWDTKNSSSRKSESLNFQRRPLIMPHEITQSMRKDEQIIIVQGHSPIRCGRAIYFRRKEMDMAAE
ANRFVKAARP

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(170-635)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 192719..202197

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Q9313_RS25260 (Q9313_25260) 189042..189269 - 228 WP_306040535 hypothetical protein -
Q9313_RS28960 189266..189535 - 270 WP_370360288 hypothetical protein -
Q9313_RS25265 (Q9313_25265) 189502..189882 - 381 WP_306040536 TetR/AcrR family transcriptional regulator -
Q9313_RS25270 (Q9313_25270) 190272..190556 - 285 WP_306040538 hypothetical protein -
Q9313_RS25275 (Q9313_25275) 190733..191281 - 549 WP_306040541 NADPH-dependent FMN reductase -
Q9313_RS25280 (Q9313_25280) 191318..191485 - 168 Protein_185 P-type DNA transfer ATPase VirB11 -
Q9313_RS25290 (Q9313_25290) 192764..193612 - 849 Protein_187 P-type DNA transfer ATPase VirB11 -
Q9313_RS25295 (Q9313_25295) 193622..194797 - 1176 WP_306040547 type IV secretion system protein VirB10 virB10
Q9313_RS25300 (Q9313_25300) 194809..195645 - 837 WP_306040727 P-type conjugative transfer protein VirB9 virB9
Q9313_RS25305 (Q9313_25305) 195666..196337 - 672 WP_306040549 virB8 family protein virB8
Q9313_RS25310 (Q9313_25310) 196342..196608 - 267 WP_306040551 hypothetical protein -
Q9313_RS25315 (Q9313_25315) 196643..197578 - 936 WP_306040553 type IV secretion system protein virB6
Q9313_RS25320 (Q9313_25320) 197585..197818 - 234 WP_306040555 EexN family lipoprotein -
Q9313_RS25325 (Q9313_25325) 197815..198513 - 699 WP_306040557 P-type DNA transfer protein VirB5 virB5
Q9313_RS25330 (Q9313_25330) 198513..200894 - 2382 WP_306040559 VirB4 family type IV secretion/conjugal transfer ATPase virb4
Q9313_RS25335 (Q9313_25335) 200887..201228 - 342 WP_306040561 type IV secretion system protein VirB3 virB3
Q9313_RS25340 (Q9313_25340) 201233..201532 - 300 WP_306040563 TrbC/VirB2 family protein virB2
Q9313_RS25345 (Q9313_25345) 201529..202197 - 669 WP_306040566 type IV secretion system protein VirB1 virB1
Q9313_RS25350 (Q9313_25350) 202200..202739 - 540 WP_306040568 hypothetical protein -
Q9313_RS25355 (Q9313_25355) 202835..203179 + 345 WP_306040570 transcriptional regulator -
Q9313_RS25360 (Q9313_25360) 203418..205061 - 1644 WP_306040573 hypothetical protein -

Host bacterium

ID   15193 GenBank   NZ_CP132304
Plasmid name   1760953|unnamed2 Incompatibility group   -
Plasmid size   205446 bp Coordinate of oriT [Strand]   153630..153658 [+]
Host baterium   Shinella sumterensis strain 1760953

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -