Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114689
Name   oriT_pMRSN791417_5 in_silico
Organism   Enterobacter hormaechei strain MRSN791417
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP132002 (7992..9697 [-], 1706 nt)
oriT length   1706 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 1706 nt

>oriT_pMRSN791417_5
GCCGTATGAATGACATCTATTTCCGTCCAATTCATCGAAACCAGTATTTTGAGGAAATGAAAGCATGATAAAGAAACTTAAAGTCATCATGTTGGCGGCAATAGTTACCGGATGTTCATCGCCACCGCCTCCCGTTCCTGTAGAGTGGGATAAAGCCGCCGACCCATTAAATACCAGTTTACCGCAATGGAGTGACAATAACGTTATAGTCCCTTCCCCGACAGTTAACGGTAAATGGACATCATCAATCAGCGCACAAAATTTCTACGATACAATATGGACTCCGGCAGTATTTTACGCAGTCGCCCATTCAACACGAATTGTCGTCACTGCACAATCCGGTACTGATTTCTTTAATACCAAAAACTGGCTCCGCCAGAATGGCGCAAAAGGCGTTATTGAATACCAGCCCGCCTTTAATTGTCTGACATGCAGAGAAACAACCATTTATTTCTCTCGCTAATCCATTTAAGCAATACAATCTAATAAGGATTTACCATGAAAAAAACCGTTATCGCGGCGGCTGTGTTATGCCTGACCGCAGGAATACCGATTTATTCATTTGCCGCTGACCCGTGCGAAGTTGTTCTTTGCATGTACGGAAAAGCTACGGGTAATAGTGGCGGGAGTGAATGCAGTTCAGCAGAACGCGCATTTTTTAAAATTAATGCATTTAAAAAACACCATCGATTTAATCCGGGTAAAACATTCGATATGCGAAAAGAATTTTTAGGCGAATGTAAAGCCGCAGACCCTGAATCAATAAGCAAAATCCTTTCAAAATTTGGCAGAGTGAAAGGATAACTGACATATCCATTTTGATATGAGAGGTTCGGAATGATAAACGAAGATTTATTCATTAAGAATATACACTCAAAAAATCAGGACAGAATATCTGTAGCTCTGGTATATGACACGTTATCAAAAGAAGCCCACAGAGGTTGTGGGCTGTATTACGAAATATACGAAAGTTGTTTTATCGGTCTTTTGCGCGATCATCTTTCAGAACTTAATGAAGCTGATGCAAATAAATTAAGACGCTATGCCGAAAGCAAAGGCACAAAAATTGATGATGCGTCCTACAGTGAAGCACTGGAAGCTGAAAGGGAGTGCAGGGCTGAAATATACCGCGAGCAAATGTAATCACACAAAATAAAAGTGTATTGTACAATACACAGACTAACAGGCGACGGGATTTTAAGGCTTCATTATTTTTGGCTTCAATTTCGAAGAAATTGTTAAGACGAAAAATAATGAAGTGGTACGCGGAAAGAAAATTCAATTTCGAAGAAATTGCTAATTTTCAGGAACCGCGCCCACCTGCGAACATTCGCCCACAGCGAATAGAGCAGGCCGGGGGGAGCCGCAAAAATTTTGCCGCCCCAGCGAGCAAAAAGGCACGCCCACGTGCCGTTTTTTGTGGGGGGCTTGCCCCGCACACCGTCGCAGCCAGGTAATATAACCCGCCCCAGCGGGGGATATTACCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCGATTTTTTCGAGCTTGCGAGGAAAAATAGGCTAAACGCAGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCAAGACGGTGTGCCGCCTTTGACCTTGGGGGTTTTGGCTCGGCGTTCAGCCGGGACATTACCCCCATGTGTCATGCAGTGAGGGCGTCCAGCCCGAACGTAATGGCACACGCCGT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


T4CP

ID   10954 GenBank   WP_041175934
Name   t4cp2_Q8Z25_RS28130_pMRSN791417_5 insolico UniProt ID   _
Length   578 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 578 a.a.        Molecular weight: 65641.81 Da        Isoelectric Point: 7.2144

>WP_041175934.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacterales]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLLAGLTLPFIFHLPREERKNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKSLDNNILFRVPLWIMTIIFTAAGLIFHIAARRLLAGEIDNLKHRMIKKSKLERNT
RTDVRKVKELLPDSIEYNPLDYIDLKKGVFIGLNKDDQPQYITIKEFKTQHAAIIGTTGSGKGVTATVLL
YQAILLGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKLNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYENGMTLRDLFNTDFVQSLREDVPAFCGELEEIALVNSINAKNGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDSVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKVDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTSTELPRATKMFQVQVKKENIELLSFEYK
QATPEKNESQEHKPSINL

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(336-537)

Helicase HerA, central domain (DUF87)

(174-275)

  Protein structure



No available structure.



ID   10955 GenBank   WP_001096398
Name   t4cp2_Q8Z25_RS28190_pMRSN791417_5 insolico UniProt ID   _
Length   912 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 912 a.a.        Molecular weight: 103084.02 Da        Isoelectric Point: 7.4625

>WP_001096398.1 MULTISPECIES: VirB3 family type IV secretion system protein [Enterobacterales]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGNAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGLEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL

  Protein domains


Predicted by InterproScan.

Type IV secretory pathway, VirB3-like protein (VirB3)

(3-79)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(269-470)

TraG P-loop domain (P-loop_TraG)

(535-813)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 6039..19876

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Q8Z25_RS28090 (Q8Z25_28100) 1571..1807 + 237 WP_025861853 type II toxin-antitoxin system CcdA family antitoxin -
Q8Z25_RS28095 (Q8Z25_28105) 1811..2125 + 315 WP_128299481 CcdB family protein -
Q8Z25_RS28100 (Q8Z25_28110) 2501..3283 + 783 WP_016603533 SprT-like domain-containing protein -
Q8Z25_RS28105 (Q8Z25_28115) 3403..4050 + 648 WP_004178130 ParA family protein -
Q8Z25_RS28110 (Q8Z25_28120) 4091..4324 + 234 WP_004178129 hypothetical protein -
Q8Z25_RS28115 (Q8Z25_28125) 4371..4703 - 333 WP_322436815 hypothetical protein -
Q8Z25_RS28120 (Q8Z25_28130) 4764..5237 - 474 WP_061586426 thermonuclease family protein -
Q8Z25_RS28125 (Q8Z25_28135) 5275..6030 - 756 WP_087638547 MobC family replication-relaxation protein -
Q8Z25_RS28130 (Q8Z25_28140) 6039..7775 - 1737 WP_041175934 type IV secretory system conjugative DNA transfer family protein virb4
Q8Z25_RS28135 (Q8Z25_28145) 8555..8860 - 306 WP_032152569 hypothetical protein -
Q8Z25_RS28140 (Q8Z25_28150) 8894..9199 - 306 WP_305871529 conjugal transfer protein -
Q8Z25_RS28145 (Q8Z25_28155) 9235..9633 - 399 WP_305871530 cag pathogenicity island Cag12 family protein -
Q8Z25_RS28150 (Q8Z25_28160) 9630..10655 - 1026 WP_000125558 P-type DNA transfer ATPase VirB11 virB11
Q8Z25_RS28155 (Q8Z25_28165) 10645..10965 - 321 WP_305871531 hypothetical protein -
Q8Z25_RS28160 (Q8Z25_28170) 10977..12221 - 1245 WP_305871532 VirB10/TraB/TrbI family type IV secretion system protein virB10
Q8Z25_RS28165 (Q8Z25_28175) 12265..13173 - 909 WP_305871526 P-type conjugative transfer protein VirB9 virB9
Q8Z25_RS28170 (Q8Z25_28180) 13170..13853 - 684 WP_004178111 type IV secretion system protein virB8
Q8Z25_RS28175 (Q8Z25_28185) 14076..15146 - 1071 WP_023213242 type IV secretion system protein virB6
Q8Z25_RS28180 (Q8Z25_28190) 15158..15382 - 225 WP_053271848 EexN family lipoprotein -
Q8Z25_RS28185 (Q8Z25_28195) 15394..16095 - 702 WP_094850971 type IV secretion system protein -
Q8Z25_RS28190 (Q8Z25_28200) 16113..18851 - 2739 WP_001096398 VirB3 family type IV secretion system protein virb4
Q8Z25_RS28195 (Q8Z25_28205) 18864..19169 - 306 WP_012457109 TrbC/VirB2 family protein virB2
Q8Z25_RS28200 (Q8Z25_28210) 19166..19876 - 711 WP_101989724 lytic transglycosylase domain-containing protein virB1
Q8Z25_RS28205 (Q8Z25_28215) 19994..20212 - 219 WP_004178172 hypothetical protein -
Q8Z25_RS28210 (Q8Z25_28220) 20217..20432 - 216 WP_004178170 hypothetical protein -
Q8Z25_RS28215 (Q8Z25_28225) 20504..20959 - 456 WP_009309974 transcription termination/antitermination NusG family protein -
Q8Z25_RS28220 (Q8Z25_28230) 21246..21584 + 339 WP_306460784 helix-turn-helix domain-containing protein -

Host bacterium

ID   15124 GenBank   NZ_CP132002
Plasmid name   pMRSN791417_5 Incompatibility group   IncFII
Plasmid size   22055 bp Coordinate of oriT [Strand]   7992..9697 [-]
Host baterium   Enterobacter hormaechei strain MRSN791417

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -