Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114688
Name   oriT_pMRSN791417_7 in_silico
Organism   Enterobacter hormaechei strain MRSN791417
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP132000 (86341..86443 [-], 103 nt)
oriT length   103 nt
IRs (inverted repeats)      89..94, 96..101  (AAATAA..TTATTT)
 28..33, 40..45  (GCAAAA..TTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 103 nt

>oriT_pMRSN791417_7
CTCACCACCAAAAGCTCCACACCCCACGCAAAATTAAAGTTTTGCTGATTGGGTATTCAAATCATGCAGTTAGTGAAAAAGTAATGTGAAATAATTTATTTTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9423 GenBank   WP_022652257
Name   traI_Q8Z25_RS27050_pMRSN791417_7 insolico UniProt ID   A0A3S5I3M3
Length   1746 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1746 a.a.        Molecular weight: 189292.49 Da        Isoelectric Point: 6.1121

>WP_022652257.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMGERWAGKGAEALGLSGGVDQKVFTKVLEGRLPDGSDLSRTQ
DGANKHRPGYDFTFSAPKSVSVMAMLGGDKRLIEAHNRAVETAIKQIEAMASTRVMTEGRSETQLTGNLV
MALFNHDTSRDQEPQLHTHAVVANATQHGDEWRTLSSDKVGKTGFIENIYANQIAFGRLYRAALKDDVAA
MGYETETVGKHGMWELKGVPTEPYSSRSRTISEAVGDDASLKSRDVAALDTRQSKQKVDPEQRMAEWMHT
LKETGFDIKAYREAADLRVVQGNIPATTPEAIDINSSVGQAIAMLSDRRARFTYSELLATTLGQLPARSG
MVEMARDGIDAAIKNEQLIPLDKEKGLFTSNIHVLDELSVSAMTRDLQRTGQADIFPDKSVPRSRSYSDA
VSVLAQDRPPVAIISGQGGAAGQRERVAELTMMAREQGRDVQIIAADRRSRSALMQDERLSGEHITDRRG
LTEGMAFTPGSTLIVDQGEKLTLKETLTLLDGALRHNVQLLIADSGQRTGTGSALTVMKEAGVSTLAWQG
GEKTQVSVISEPDRRQRYDRLAADFARSVRAGEHSVAQVTGPREQAVLAGVVREALKAEKVLGEREVSIT
TLEPVWLDSRHQQVRDHYREGMVMERWNAEERSRERFVIDRVTGRNNSLTLRNAQGETRVTRLSELNSSW
SLYRTGTLQVAEGDRLAVLGQAQGTRLRGGDTVTVKALGEAGLVVSLPGRKADVVLPAGDSPFTAAKVVQ
GWVESPGRSVSDSATVFASLSQRELDNTTLNKLALSGRAVRLYSAQTAQKTTEKLSRQSAWSVVSEQIKD
AAGHDRLDDALAHQKAGLHTPEQQAIHLAIPVLEGNGLAFTKPQLMAAAKEFEQDSLSLQAIEKEADRQV
RSGDLLSVPVSPGNGLQLLVSRQSYNAEKSIIRHVLEGKEAVTPLMDKVPDAQLAGLTDGQRNATRMILE
SPDRFTLVQGYAGVGKTTQFRAVMSAIGTLPEREQPRVIGVAPTHRAVSEMRDAGVPETQTLAAFIHDTQ
QQLRGGERTDFSNVLFLVDESSMVGNADMAKAYSLIAGGGGRAVSSGDTDQLQSIAPGQPFRLLQKRSAI
DVAVMQEIVRQTPELKPAVYSLISRDIGSALTTIESVVPAQVPRRADAWQPDSSVMEFSREREEAIAKAV
AAGDLMPGGQPATLLEAIVKDYTGRTSEAQAQTIVITALNADRRQVNAMIHDARQGAGEVGEKEVTLPVL
TPANIRDGELRRMATWEASRDSLVLLDSTYYSIEALDGEAHLVMLKDAQGNTRSLSPAQAATEGVTLYRQ
DTITVSEGDRMRFSKSDNERGFVANSVWSVSEIKGDSVTLSDGKQTRTLTPSAEQAEQHIDLAYAVTVEG
SQGASEPFSISLQGTDGGRKRMVSFESAYVALSRMKQHAQVYTDNRDKWVAAMEKSQAKSTAHDILEPRG
DRAVANAARLTATAKALGEVPAGRAALRQAGLQPEGSMAKYISPGRKYPQPHVALPAFDRNGRKAGVWLS
ALTSGDGQLKGLAGEGRVMGSGDAAFAGLQASRNGESLLARDMEEGVRLARENPQSGVVVRLEGEARPWN
PGAITGGRVWADGLPDATGTQPAETVPPEVLAQQAREAEVQRELDKRAEEAVREMARSGDRPAGGADVAV
RDVVRDMDRIKETPASPLTLPDAPEERRRDEAVSQVVRESVQRDRLQQMERETVRDLEREKTLGGD

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(631-710)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(573-625)

AAA domain (AAA_30)

(967-1148)

TrwC relaxase (TrwC)

(10-283)

DNA helicase TraI (TraI_C)

(1430-1551)


  Protein structure



No available structure.




T4CP

ID   10952 GenBank   WP_022652256
Name   traD_Q8Z25_RS27055_pMRSN791417_7 insolico UniProt ID   _
Length   736 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 736 a.a.        Molecular weight: 82566.18 Da        Isoelectric Point: 5.1609

>WP_022652256.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIASMRIRMFSQIANIIVYCLVILFGIIVAITLSVKLTWQTFVNGMVYYWCQTLAPMR
DIFKSQPVYTIRYYGHELKYNAQQILQDKYIQWCADQVWTAFILAAIIAAVVCVITFFVTTWILGHQGKQ
QSEDEITGGRQLTDDPKAVAKMLRRDGLASDIKIGELPLIKDSEIQNFCLHGTVGAGKSEVIRRLMNYAR
QRGDMVIVYDRSCEFVKSYYDPATDKILNPLDARCAAWDLWRECLTQPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDKDRSYSKLVDTLLSIKIEKLRSYLKNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIENNGESFTIRDWMRGVREDGKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVIGIQSYAQLEDIYGQKPAETLFDVLNTRAFFRSPSRNIA
EFAAGEIGEKEILKASEQYSYGVDPVRDGTSVGKDMERVTLVSYSDVQTLPDLSCYVTLPGPYPAVKMAL
KYQQRPKVAPEFIPRTIDGKAEERLNAALAAREHESRSIAMLFEPDADVVAPEGGAENVGQPQQPQQPQQ
PQQPQQPQQPQQPQQLTPAPRAADAASVAGAAGAGGVEPELKTKAEEAEQLPPGISESGEVVDMAAWEAW
QAEQDELSPQERQRREEVNINVHRAPEKDLEPGGYI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.



ID   10953 GenBank   WP_008324134
Name   traC_Q8Z25_RS27130_pMRSN791417_7 insolico UniProt ID   _
Length   876 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 876 a.a.        Molecular weight: 98841.51 Da        Isoelectric Point: 5.7676

>WP_008324134.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacterales]
MSNPLDSVTRTVNSLLTALKMPDASSEANDVLGQMRFPQFSRVLPYRDYDAETGLFMNEKTMGFMLEAQP
INGANETIVSSLENLMRTKLPREVPVAVHLVSSHLVGQDIEYGLRELSWTGEKADTFNAITRAYYLRAAE
EGFPLPDHLNLPLTLRNYRVYISCCVPRKRGSKASVMEMENQVKIIRASLQGAQIATRSVDADAFIRIAG
EMINHNPDALYPTERELDPLKDLNYQCVDDTFDLKVKADYMTLGLRGNGRNSTARIMNFQLTKNPELAFL
WNMADNYSNLLNPELSVSCPFILTLVLSVEDQVKAQNEANLKFMDLEKKSKTSYAKFFPGVAQEAKEWGD
LRQRLSSGQSSLVSYFLNVTLFCRDDQETALKVEQDVLNSFRKNGFELISPRFNHMRNFLVNLPFMAGGG
LFKQLRESGVLQRAESFNVANLMPVVADSPLATSGLLAPTYRNQLAFIDLFSRGMGNTNFNMAVCGTSGA
GKTGLIQPLIRSVIDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLKFNPFANVTDDTIDEMAERLRDQL
SVMASPNGNLDEVHEGLLLKAVKATWLTKKNQARIDDVVDYLMMARDSEEYSGSPTIRSRLDEMVVLLTQ
YTRDGVYGSYFNSDEPSLKDEARMVVLELGGLEDRPSLLVAVMFSLILYIENRMYRTPRSLKKLNVIDEG
WRLLDFKNNKVGAFIEKGYRTARRHTGSYITITQNIVDFDSETASSAARAAWGNSSYKIILKQSAKEFAK
YNQLYPDQYSQLQRDMIKGFGSAKDNWFSSFMLSVEHLTSWHRLFVDPLSRAMYSSDGPDFEFLQQRRRE
GLSIHEAVWELAWKKSGPEMAALERWLHEHEGKTIS

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(289-446)

TraG P-loop domain (P-loop_TraG)

(470-765)

TraC protein (TraC_F_IV)

(38-276)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 61857..86991

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Q8Z25_RS27055 (Q8Z25_27065) 61857..64067 - 2211 WP_022652256 type IV conjugative transfer system coupling protein TraD virb4
Q8Z25_RS27060 (Q8Z25_27070) 64201..64875 - 675 WP_008324909 tetratricopeptide repeat protein -
Q8Z25_RS27065 (Q8Z25_27075) 64950..65381 - 432 WP_008324118 entry exclusion protein -
Q8Z25_RS27070 (Q8Z25_27080) 65403..68225 - 2823 WP_008324119 conjugal transfer mating-pair stabilization protein TraG traG
Q8Z25_RS27075 (Q8Z25_27085) 68225..69595 - 1371 WP_016247527 conjugal transfer pilus assembly protein TraH traH
Q8Z25_RS27080 (Q8Z25_27090) 69582..70010 - 429 WP_008324122 hypothetical protein -
Q8Z25_RS27085 (Q8Z25_27095) 70003..70557 - 555 WP_008324125 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
Q8Z25_RS27090 (Q8Z25_27100) 70547..70795 - 249 WP_008324126 type-F conjugative transfer system pilin chaperone TraQ -
Q8Z25_RS27095 (Q8Z25_27105) 70812..71561 - 750 WP_008324127 type-F conjugative transfer system pilin assembly protein TraF traF
Q8Z25_RS27100 (Q8Z25_27110) 71592..71780 - 189 WP_008324128 conjugal transfer protein TrbE -
Q8Z25_RS27105 (Q8Z25_27115) 71812..73665 - 1854 WP_022652255 type-F conjugative transfer system mating-pair stabilization protein TraN traN
Q8Z25_RS27110 (Q8Z25_27120) 73662..74291 - 630 WP_008324130 type-F conjugative transfer system pilin assembly protein TrbC trbC
Q8Z25_RS27115 (Q8Z25_27125) 74308..75294 - 987 WP_032160671 conjugal transfer pilus assembly protein TraU traU
Q8Z25_RS27120 (Q8Z25_27130) 75297..75944 - 648 WP_008324132 type-F conjugative transfer system protein TraW traW
Q8Z25_RS27125 (Q8Z25_27135) 75944..76291 - 348 WP_008324133 type-F conjugative transfer system protein TrbI -
Q8Z25_RS27130 (Q8Z25_27140) 76288..78918 - 2631 WP_008324134 type IV secretion system protein TraC virb4
Q8Z25_RS27135 (Q8Z25_27145) 78911..79477 - 567 WP_008324135 conjugal transfer pilus-stabilizing protein TraP -
Q8Z25_RS27140 (Q8Z25_27150) 79467..79868 - 402 WP_008324137 hypothetical protein -
Q8Z25_RS27145 (Q8Z25_27155) 79852..80037 - 186 WP_022652254 hypothetical protein -
Q8Z25_RS27150 (Q8Z25_27160) 80041..80265 - 225 WP_008324141 TraR/DksA C4-type zinc finger protein -
Q8Z25_RS27155 (Q8Z25_27165) 80385..80924 - 540 WP_008324154 type IV conjugative transfer system lipoprotein TraV traV
Q8Z25_RS27160 (Q8Z25_27170) 80946..82340 - 1395 WP_008324156 F-type conjugal transfer pilus assembly protein TraB traB
Q8Z25_RS27165 (Q8Z25_27175) 82327..83070 - 744 WP_008324157 type-F conjugative transfer system secretin TraK traK
Q8Z25_RS27170 (Q8Z25_27180) 83057..83623 - 567 WP_008324158 type IV conjugative transfer system protein TraE traE
Q8Z25_RS27175 (Q8Z25_27185) 83638..83943 - 306 WP_008324160 type IV conjugative transfer system protein TraL traL
Q8Z25_RS27180 (Q8Z25_27190) 84094..84444 - 351 WP_008324162 type IV conjugative transfer system pilin TraA -
Q8Z25_RS27185 (Q8Z25_27195) 84502..84726 - 225 WP_008324165 TraY domain-containing protein -
Q8Z25_RS27190 (Q8Z25_27200) 84813..85502 - 690 WP_008324166 hypothetical protein -
Q8Z25_RS27195 (Q8Z25_27205) 85691..86083 - 393 WP_008324167 conjugal transfer relaxosome DNA-binding protein TraM -
Q8Z25_RS27200 (Q8Z25_27210) 86515..86991 + 477 WP_008324168 transglycosylase SLT domain-containing protein virB1
Q8Z25_RS27205 (Q8Z25_27215) 87038..87568 - 531 WP_008324170 antirestriction protein -
Q8Z25_RS27210 (Q8Z25_27220) 88195..89025 - 831 WP_008324171 N-6 DNA methylase -
Q8Z25_RS27215 (Q8Z25_27225) 89068..89331 - 264 WP_008324174 hypothetical protein -
Q8Z25_RS27220 (Q8Z25_27230) 89417..89761 - 345 WP_008324177 hypothetical protein -
Q8Z25_RS27225 (Q8Z25_27235) 89808..90095 - 288 WP_008324178 hypothetical protein -
Q8Z25_RS27230 (Q8Z25_27240) 90149..90523 - 375 WP_008324180 hypothetical protein -
Q8Z25_RS27235 (Q8Z25_27245) 91150..91509 - 360 WP_008324181 hypothetical protein -
Q8Z25_RS27240 (Q8Z25_27250) 91571..91894 - 324 WP_008324183 hypothetical protein -

Host bacterium

ID   15123 GenBank   NZ_CP132000
Plasmid name   pMRSN791417_7 Incompatibility group   IncFIB
Plasmid size   117297 bp Coordinate of oriT [Strand]   86341..86443 [-]
Host baterium   Enterobacter hormaechei strain MRSN791417

Cargo genes

Drug resistance gene   tet(D)
Virulence gene   -
Metal resistance gene   merR, merT, merP, merA, merD, merE, merC
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -