Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 114684
• Name: oriT_pSR3
• Organism: Aminobacter sp. SR38
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP062115 (80942..80988 [+], 47 nt)
• oriT length: 47 nt
• IRs (inverted repeats): 22..27, 32..37 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 47 nt

>oriT_pSR3
GGGTCCAAGGGCGCAATTATACGTCGCTGACGCGACGACTTGCTTAG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_IG197_RS31405_pSR3

• ID: 9420
• GenBank: WP_192320957
• Name: traA_IG197_RS31405_pSR3
• UniProt ID: _
• Length: 1103 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1103 a.a.
• Molecular weight: 122381.58 Da
• Isoelectric Point: 9.8043

>WP_192320957.1 Ti-type conjugative transfer relaxase TraA [Aminobacter sp. SR38]
MAVPHFSVSIVARGAGRSAVLSAAYRHCAKMDYEREARTIDYTRKQGLLHEEFVVPPDAPEWLRSMIADR
SVSGASEAFWNKVESFERRTDAQLAKDLTIALPIELTAAQNIALVQEFVKRHITAKSMVADWVFHDTAGN
PHVHLMTTLRPLTEEGFGAKKVALLDPDGDPIRNAAGKIVYELWAGSVDDFNVLRDGWFACQNSHLARAG
LDIRVDGRSYEKQGIALTPTIHLGVGTKAIERQSTTASQDVSLERLELQEERRAENAHRIQRNPEIVLDL
ITREKSVFDERDVAKVLHRYIDDARLFQSLLARILQSPQTLRLDRERIDFTTGLRTPAKYTTRALIRLEA
EMAHRAIWLSYRSSHGIEDAVLKATFARHSRLSQEQMTAIQHIAGGERIAAVIGRAGAGKTTMMKAAREA
WEAAGYRVVGAALAGKAADGLEKEANIASRTLSAWELRWSQGRNQLDDKTIFVLDEAGMVSSRQMAHVIE
TATRAGAKLVLVGDPEQLPPIEAGAAFRAIADRIGYAELETIYRQREQWMRDASLDLARGNVGKAADVYG
ANGKVMGSELKAQAVSNLISDWSRDYDSAKSSLILAHLRRDVRMLNELARVRLIGRGIVDEGCVFKTADG
NRNFAVGDQIVFLKNEGSLGVKNGMLGKVVQATSGRIVAEIGDGERRRQVAVEQHFYNSVDHGYATTIHK
SQGATVDQVKVLASLSLDRHLTYVAMTRHREDLKLYYGRRSFAKAGGLIPILSRRNSKETTLDYETSTFY
RAALRFAEARGLHLMNVARTLVGDRLAWTVRQTQKLSELGNRLAEVARKLGLTPRAITTSTHIVAKEVKP
MVSGITTFPNTVEQTVEDKLVADPVLKTLWEEVSMRFRHVYAQPEAAFKAMNVDAMLKEQNVAQSTIAMI
AGEPESFGALKGKSGMFASKVDKQDRDRALKNVEALADGLRDYLRQRAETEQRTLAAQMAVRKQVSIEIP
ALSASAKTVLERVRDAIDRNDLPAGLEYALSDKMVKAELEGFAKAISERFGERTFLPLAARDADGETFKR
LTAGMTNTQKAEIQSAWSTMRVVQQLATHERTTLALKEADAMRQTKSQGLSLK

Protein domains

Predicted by InterproScan.

• AAA domain (AAA_30) (382-566)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (859-955)

• Viral (Superfamily 1) RNA helicase (Viral_helicase1) (691-736)

• MobA/MobL family (MobA_MobL) (17-242)

Protein structure

No available structure.

T4CP

traG_IG197_RS31390_pSR3

• ID: 10946
• GenBank: WP_192320954
• Name: traG_IG197_RS31390_pSR3
• UniProt ID: _
• Length: 640 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 640 a.a.
• Molecular weight: 69077.06 Da
• Isoelectric Point: 9.6462

>WP_192320954.1 Ti-type conjugative transfer system protein TraG [Aminobacter sp. SR38]
MSATRIVVAITPVVLMVGATILTTGMGQGLSDFGKSDAAKLMLGQIGLALPYLLAAVTGIIFLFAAAGSV
NIRSVGWGVVAGSGTTILIAGLRETTRLASFAGQVPAGKSIFAYLDPATMIGAAALLMVGCFALRVALIG
NAAFARTEPKRIRGKRAMHGEADWMKLVEAEKLFPAAGGIVIGERYRVDRDSVAAHSFRADRSETWGAGG
KSPLLCFDGAFGSSHGIVFAGSGGFKTTSVTIPTALKWGGTLIVLDPSNEVAPMVSKHREDVGRRIRILD
PKHPQTGFNALDWIGKFGGTKEEDIAAVASWIMSDSGRASGVRDDFFRASGLQLLTAMIADVCLSGHTDI
DHQTLRQVRANLSEPEPKLRQRLQDIYDNSGSDFVKENVAAFVNMTPETFSGVYANAIKETHWLSYPNYA
ALVSSSTFSTEDIASGKTDVFINVDLKTLETHAGLARVIIGSFLNAIYNRDGAVNGRALFLLDEVARLGY
IKILETARDAGRKYGITLTMIYQSIGQLRETYGGRDASSKWFESASWISFAAINDPETADYISKRCGMAT
VEIDQVSRTSQANGSSRTRSSQLAARPLIQPHEVLLMRADEQIVFTAGNAPLRCGRAVWFRRKDMRSVVG
TNRYHRERKT

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (163-633)

Protein structure

No available structure.

Host bacterium

• ID: 15119
• GenBank: NZ_CP062115
• Plasmid name: pSR3
• Incompatibility group: -
• Plasmid size: 170880 bp
• Coordinate of oriT [Strand]: 80942..80988 [+]
• Host baterium: Aminobacter sp. SR38

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: fixB, fixA
• Anti-CRISPR: -