Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114679
Name   oriT_MGH 14|unnamed2 in_silico
Organism   Enterobacter sp. MGH 14
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP072963 (144182..144284 [-], 103 nt)
oriT length   103 nt
IRs (inverted repeats)      89..94, 96..101  (AAATAA..TTATTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 103 nt

>oriT_MGH 14|unnamed2
CTCACCACCAAAAGCTCCACACCCCACGCAAAATTAAAGTTTTTCTGATTGGGTATTCAAATCATGCAGTTAGTGAAAAAGTAATGTGAAATAATTTATTTTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9417 GenBank   WP_022652257
Name   traI_IBG17_RS26040_MGH 14|unnamed2 insolico UniProt ID   A0A3S5I3M3
Length   1746 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1746 a.a.        Molecular weight: 189292.49 Da        Isoelectric Point: 6.1121

>WP_022652257.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMGERWAGKGAEALGLSGGVDQKVFTKVLEGRLPDGSDLSRTQ
DGANKHRPGYDFTFSAPKSVSVMAMLGGDKRLIEAHNRAVETAIKQIEAMASTRVMTEGRSETQLTGNLV
MALFNHDTSRDQEPQLHTHAVVANATQHGDEWRTLSSDKVGKTGFIENIYANQIAFGRLYRAALKDDVAA
MGYETETVGKHGMWELKGVPTEPYSSRSRTISEAVGDDASLKSRDVAALDTRQSKQKVDPEQRMAEWMHT
LKETGFDIKAYREAADLRVVQGNIPATTPEAIDINSSVGQAIAMLSDRRARFTYSELLATTLGQLPARSG
MVEMARDGIDAAIKNEQLIPLDKEKGLFTSNIHVLDELSVSAMTRDLQRTGQADIFPDKSVPRSRSYSDA
VSVLAQDRPPVAIISGQGGAAGQRERVAELTMMAREQGRDVQIIAADRRSRSALMQDERLSGEHITDRRG
LTEGMAFTPGSTLIVDQGEKLTLKETLTLLDGALRHNVQLLIADSGQRTGTGSALTVMKEAGVSTLAWQG
GEKTQVSVISEPDRRQRYDRLAADFARSVRAGEHSVAQVTGPREQAVLAGVVREALKAEKVLGEREVSIT
TLEPVWLDSRHQQVRDHYREGMVMERWNAEERSRERFVIDRVTGRNNSLTLRNAQGETRVTRLSELNSSW
SLYRTGTLQVAEGDRLAVLGQAQGTRLRGGDTVTVKALGEAGLVVSLPGRKADVVLPAGDSPFTAAKVVQ
GWVESPGRSVSDSATVFASLSQRELDNTTLNKLALSGRAVRLYSAQTAQKTTEKLSRQSAWSVVSEQIKD
AAGHDRLDDALAHQKAGLHTPEQQAIHLAIPVLEGNGLAFTKPQLMAAAKEFEQDSLSLQAIEKEADRQV
RSGDLLSVPVSPGNGLQLLVSRQSYNAEKSIIRHVLEGKEAVTPLMDKVPDAQLAGLTDGQRNATRMILE
SPDRFTLVQGYAGVGKTTQFRAVMSAIGTLPEREQPRVIGVAPTHRAVSEMRDAGVPETQTLAAFIHDTQ
QQLRGGERTDFSNVLFLVDESSMVGNADMAKAYSLIAGGGGRAVSSGDTDQLQSIAPGQPFRLLQKRSAI
DVAVMQEIVRQTPELKPAVYSLISRDIGSALTTIESVVPAQVPRRADAWQPDSSVMEFSREREEAIAKAV
AAGDLMPGGQPATLLEAIVKDYTGRTSEAQAQTIVITALNADRRQVNAMIHDARQGAGEVGEKEVTLPVL
TPANIRDGELRRMATWEASRDSLVLLDSTYYSIEALDGEAHLVMLKDAQGNTRSLSPAQAATEGVTLYRQ
DTITVSEGDRMRFSKSDNERGFVANSVWSVSEIKGDSVTLSDGKQTRTLTPSAEQAEQHIDLAYAVTVEG
SQGASEPFSISLQGTDGGRKRMVSFESAYVALSRMKQHAQVYTDNRDKWVAAMEKSQAKSTAHDILEPRG
DRAVANAARLTATAKALGEVPAGRAALRQAGLQPEGSMAKYISPGRKYPQPHVALPAFDRNGRKAGVWLS
ALTSGDGQLKGLAGEGRVMGSGDAAFAGLQASRNGESLLARDMEEGVRLARENPQSGVVVRLEGEARPWN
PGAITGGRVWADGLPDATGTQPAETVPPEVLAQQAREAEVQRELDKRAEEAVREMARSGDRPAGGADVAV
RDVVRDMDRIKETPASPLTLPDAPEERRRDEAVSQVVRESVQRDRLQQMERETVRDLEREKTLGGD

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(631-710)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(573-625)

AAA domain (AAA_30)

(967-1148)

TrwC relaxase (TrwC)

(10-283)

DNA helicase TraI (TraI_C)

(1430-1551)


  Protein structure



No available structure.




T4CP

ID   10943 GenBank   WP_022652256
Name   traD_IBG17_RS26045_MGH 14|unnamed2 insolico UniProt ID   _
Length   736 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 736 a.a.        Molecular weight: 82566.18 Da        Isoelectric Point: 5.1609

>WP_022652256.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIASMRIRMFSQIANIIVYCLVILFGIIVAITLSVKLTWQTFVNGMVYYWCQTLAPMR
DIFKSQPVYTIRYYGHELKYNAQQILQDKYIQWCADQVWTAFILAAIIAAVVCVITFFVTTWILGHQGKQ
QSEDEITGGRQLTDDPKAVAKMLRRDGLASDIKIGELPLIKDSEIQNFCLHGTVGAGKSEVIRRLMNYAR
QRGDMVIVYDRSCEFVKSYYDPATDKILNPLDARCAAWDLWRECLTQPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDKDRSYSKLVDTLLSIKIEKLRSYLKNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIENNGESFTIRDWMRGVREDGKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVIGIQSYAQLEDIYGQKPAETLFDVLNTRAFFRSPSRNIA
EFAAGEIGEKEILKASEQYSYGVDPVRDGTSVGKDMERVTLVSYSDVQTLPDLSCYVTLPGPYPAVKMAL
KYQQRPKVAPEFIPRTIDGKAEERLNAALAAREHESRSIAMLFEPDADVVAPEGGAENVGQPQQPQQPQQ
PQQPQQPQQPQQPQQLTPAPRAADAASVAGAAGAGGVEPELKTKAEEAEQLPPGISESGEVVDMAAWEAW
QAEQDELSPQERQRREEVNINVHRAPEKDLEPGGYI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.



ID   10944 GenBank   WP_008324134
Name   traC_IBG17_RS26120_MGH 14|unnamed2 insolico UniProt ID   _
Length   876 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 876 a.a.        Molecular weight: 98841.51 Da        Isoelectric Point: 5.7676

>WP_008324134.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacterales]
MSNPLDSVTRTVNSLLTALKMPDASSEANDVLGQMRFPQFSRVLPYRDYDAETGLFMNEKTMGFMLEAQP
INGANETIVSSLENLMRTKLPREVPVAVHLVSSHLVGQDIEYGLRELSWTGEKADTFNAITRAYYLRAAE
EGFPLPDHLNLPLTLRNYRVYISCCVPRKRGSKASVMEMENQVKIIRASLQGAQIATRSVDADAFIRIAG
EMINHNPDALYPTERELDPLKDLNYQCVDDTFDLKVKADYMTLGLRGNGRNSTARIMNFQLTKNPELAFL
WNMADNYSNLLNPELSVSCPFILTLVLSVEDQVKAQNEANLKFMDLEKKSKTSYAKFFPGVAQEAKEWGD
LRQRLSSGQSSLVSYFLNVTLFCRDDQETALKVEQDVLNSFRKNGFELISPRFNHMRNFLVNLPFMAGGG
LFKQLRESGVLQRAESFNVANLMPVVADSPLATSGLLAPTYRNQLAFIDLFSRGMGNTNFNMAVCGTSGA
GKTGLIQPLIRSVIDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLKFNPFANVTDDTIDEMAERLRDQL
SVMASPNGNLDEVHEGLLLKAVKATWLTKKNQARIDDVVDYLMMARDSEEYSGSPTIRSRLDEMVVLLTQ
YTRDGVYGSYFNSDEPSLKDEARMVVLELGGLEDRPSLLVAVMFSLILYIENRMYRTPRSLKKLNVIDEG
WRLLDFKNNKVGAFIEKGYRTARRHTGSYITITQNIVDFDSETASSAARAAWGNSSYKIILKQSAKEFAK
YNQLYPDQYSQLQRDMIKGFGSAKDNWFSSFMLSVEHLTSWHRLFVDPLSRAMYSSDGPDFEFLQQRRRE
GLSIHEAVWELAWKKSGPEMAALERWLHEHEGKTIS

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(289-446)

TraG P-loop domain (P-loop_TraG)

(470-765)

TraC protein (TraC_F_IV)

(38-276)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 119698..144832

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IBG17_RS26045 119698..121908 - 2211 WP_022652256 type IV conjugative transfer system coupling protein TraD virb4
IBG17_RS26050 122042..122716 - 675 WP_008324909 tetratricopeptide repeat protein -
IBG17_RS26055 122791..123222 - 432 WP_008324118 entry exclusion protein -
IBG17_RS26060 123244..126066 - 2823 WP_008324119 conjugal transfer mating-pair stabilization protein TraG traG
IBG17_RS26065 126066..127436 - 1371 WP_016247527 conjugal transfer pilus assembly protein TraH traH
IBG17_RS26070 127423..127851 - 429 WP_008324122 hypothetical protein -
IBG17_RS26075 127844..128398 - 555 WP_008324125 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
IBG17_RS26080 128388..128636 - 249 WP_008324126 type-F conjugative transfer system pilin chaperone TraQ -
IBG17_RS26085 128653..129402 - 750 WP_008324127 type-F conjugative transfer system pilin assembly protein TraF traF
IBG17_RS26090 129433..129621 - 189 WP_008324128 conjugal transfer protein TrbE -
IBG17_RS26095 129653..131506 - 1854 WP_022652255 type-F conjugative transfer system mating-pair stabilization protein TraN traN
IBG17_RS26100 131503..132132 - 630 WP_008324130 type-F conjugative transfer system pilin assembly protein TrbC trbC
IBG17_RS26105 132149..133135 - 987 WP_032160671 conjugal transfer pilus assembly protein TraU traU
IBG17_RS26110 133138..133785 - 648 WP_008324132 type-F conjugative transfer system protein TraW traW
IBG17_RS26115 133785..134132 - 348 WP_008324133 type-F conjugative transfer system protein TrbI -
IBG17_RS26120 134129..136759 - 2631 WP_008324134 type IV secretion system protein TraC virb4
IBG17_RS26125 136752..137318 - 567 WP_008324135 conjugal transfer pilus-stabilizing protein TraP -
IBG17_RS26130 137308..137709 - 402 WP_008324137 hypothetical protein -
IBG17_RS26135 137693..137878 - 186 WP_022652254 hypothetical protein -
IBG17_RS26140 137882..138106 - 225 WP_008324141 TraR/DksA C4-type zinc finger protein -
IBG17_RS26145 138226..138765 - 540 WP_008324154 type IV conjugative transfer system lipoprotein TraV traV
IBG17_RS26150 138787..140181 - 1395 WP_008324156 F-type conjugal transfer pilus assembly protein TraB traB
IBG17_RS26155 140168..140911 - 744 WP_008324157 type-F conjugative transfer system secretin TraK traK
IBG17_RS26160 140898..141464 - 567 WP_008324158 type IV conjugative transfer system protein TraE traE
IBG17_RS26165 141479..141784 - 306 WP_008324160 type IV conjugative transfer system protein TraL traL
IBG17_RS26170 141935..142285 - 351 WP_008324162 type IV conjugative transfer system pilin TraA -
IBG17_RS26175 142343..142567 - 225 WP_008324165 TraY domain-containing protein -
IBG17_RS26180 142654..143343 - 690 WP_008324166 hypothetical protein -
IBG17_RS26185 143532..143924 - 393 WP_008324167 conjugal transfer relaxosome DNA-binding protein TraM -
IBG17_RS26190 144356..144832 + 477 WP_008324168 transglycosylase SLT domain-containing protein virB1
IBG17_RS26195 144879..145409 - 531 WP_008324170 antirestriction protein -
IBG17_RS26200 146036..146866 - 831 WP_022652253 N-6 DNA methylase -
IBG17_RS26205 146909..147172 - 264 WP_008324174 hypothetical protein -
IBG17_RS26210 147258..147602 - 345 WP_022652252 hypothetical protein -
IBG17_RS26215 147649..147936 - 288 WP_008324178 hypothetical protein -
IBG17_RS26220 147990..148364 - 375 WP_008324180 hypothetical protein -
IBG17_RS26225 149085..149351 - 267 WP_022652251 hypothetical protein -
IBG17_RS26230 149413..149736 - 324 WP_008324183 hypothetical protein -

Host bacterium

ID   15114 GenBank   NZ_CP072963
Plasmid name   MGH 14|unnamed2 Incompatibility group   IncFIB
Plasmid size   199350 bp Coordinate of oriT [Strand]   144182..144284 [-]
Host baterium   Enterobacter sp. MGH 14

Cargo genes

Drug resistance gene   tet(D), blaTEM-1A, aph(3')-Ia
Virulence gene   -
Metal resistance gene   merE, merD, merA, merC, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -