Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 114678 |
| Name | oriT_HC6|p1  |
| Organism | Raoultella sp. HC6 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP093277 (79861..79910 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | 33..34 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_HC6|p1
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 9416 | GenBank | WP_241723813 |
| Name | traI_MMW58_RS28850_HC6|p1 |
UniProt ID | _ |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190272.95 Da Isoelectric Point: 6.1047
>WP_241723813.1 conjugative transfer relaxase/helicase TraI [Raoultella sp. HC6]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGLEGKPVDKAVFTEILKGKLPDGSDLTRM
QDGTNKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTKALQQVETLAATRVMTDGKSETVLTGNL
IVAKFTHDTNRNEEPQLHTHAVVMNATQNGDKWQSLGTDTVGKTGFIENVYANQIAFGKIYREELKPLVE
ILGYKTEVVGKHGMWEMNEVPVEPFSTRTQEVREAAGPDASLKSRDVAALDTRKSKAAIDPAEKMVEWMN
TMKETGFDMRSYREDADKRAAELASAPVSPVKMDGPDISDVVTKAIASLSDRKVQFTYADQLARTVSQLE
AKPGVFEQARTGIDAAIEREQLIPLDREKGLFTSNIHVLDELSVKALSQEVQRQNHVSVTPDASVVRQAP
FSDAVSVLAQDRPAMGIVSGQGGAAGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDARLAGEAVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQVTADIISEPDKGARYSLLAQEFAASVREGQESVAQISGTREQNVLNSVIRDTLKNEGVLGEKE
MTVTALTPVWLDSKSRGVRDYYREGMVMERWDPETRKHDRFVIDRVTASSNMLTLKDKEGERLDMKVSAV
DSQWTLFRADKLPVAEGERLAVLGKIPDTRLKGGESITVLKSGDGQLTVQRPGQKTTQSLSVGSSPFDGI
KVGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTMEKLARHTAFSVVSE
QLKARSGETDLDAAIAQQKAGLHTPAEQAIHLSVPLLESDDLTFTRPQLLATALETGGGKVSMADIDTTI
QAQIRSGQLLNVSVSSGHGNDLLISRQTWDAEKSILTRVLEGKDAVAPLMDRVPASLMTGLTAGQRASTR
MILETPDRFTVVQGYAGVGKTTQFRAVTSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLLRNGQTPDFSNTLFLIDESSMVGLSDKAKALSLIAAGGGRAVSSGDTDQLQSISPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIDRDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTRAQEKAIQ
EALKKGESVPAGQPATLYEALVKDYAGRTPEAQSQTLVITHLNKDRRALNGLIHDARRENGETGKEEITL
PVLVTSNIRDGELRRLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRREDITVSEGDRMRFSKSDPERGYVANSVWEVKSVSGDSVTLSDGKTSRTLNPKADEAQRHIDLAYAIT
AHGAQGASEPFAIALEGVAEGRKAMASFESAYVGLSRMKQHVQVYTDSREGWIKAINASPSKATAHDILA
PRNDRAVHTAEQLFSRARPMNETAAGRAALQQSGLAKGISPGKFISPGKQYPQPHVALPVYDKNGKEAGI
WLSSLTDSDGRLQVMGGEGRVMGNEDARFVALQGSRNGESLLAGNMGEGVRVARDNPDSGVVVRLAGDDR
PWNPGAITGGRVWADPIPVVSPEAAGTDIPLPPEVLAQRAAEEAQRQNMEKQAEQTAREVAGDGRKAGES
EDRVKEVIGDVIRGLEREKPGEEKMTLPDDPQTRKQEAAVQQVAQESLQRERLQQMERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGLEGKPVDKAVFTEILKGKLPDGSDLTRM
QDGTNKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTKALQQVETLAATRVMTDGKSETVLTGNL
IVAKFTHDTNRNEEPQLHTHAVVMNATQNGDKWQSLGTDTVGKTGFIENVYANQIAFGKIYREELKPLVE
ILGYKTEVVGKHGMWEMNEVPVEPFSTRTQEVREAAGPDASLKSRDVAALDTRKSKAAIDPAEKMVEWMN
TMKETGFDMRSYREDADKRAAELASAPVSPVKMDGPDISDVVTKAIASLSDRKVQFTYADQLARTVSQLE
AKPGVFEQARTGIDAAIEREQLIPLDREKGLFTSNIHVLDELSVKALSQEVQRQNHVSVTPDASVVRQAP
FSDAVSVLAQDRPAMGIVSGQGGAAGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDARLAGEAVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQVTADIISEPDKGARYSLLAQEFAASVREGQESVAQISGTREQNVLNSVIRDTLKNEGVLGEKE
MTVTALTPVWLDSKSRGVRDYYREGMVMERWDPETRKHDRFVIDRVTASSNMLTLKDKEGERLDMKVSAV
DSQWTLFRADKLPVAEGERLAVLGKIPDTRLKGGESITVLKSGDGQLTVQRPGQKTTQSLSVGSSPFDGI
KVGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTMEKLARHTAFSVVSE
QLKARSGETDLDAAIAQQKAGLHTPAEQAIHLSVPLLESDDLTFTRPQLLATALETGGGKVSMADIDTTI
QAQIRSGQLLNVSVSSGHGNDLLISRQTWDAEKSILTRVLEGKDAVAPLMDRVPASLMTGLTAGQRASTR
MILETPDRFTVVQGYAGVGKTTQFRAVTSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLLRNGQTPDFSNTLFLIDESSMVGLSDKAKALSLIAAGGGRAVSSGDTDQLQSISPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIDRDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTRAQEKAIQ
EALKKGESVPAGQPATLYEALVKDYAGRTPEAQSQTLVITHLNKDRRALNGLIHDARRENGETGKEEITL
PVLVTSNIRDGELRRLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRREDITVSEGDRMRFSKSDPERGYVANSVWEVKSVSGDSVTLSDGKTSRTLNPKADEAQRHIDLAYAIT
AHGAQGASEPFAIALEGVAEGRKAMASFESAYVGLSRMKQHVQVYTDSREGWIKAINASPSKATAHDILA
PRNDRAVHTAEQLFSRARPMNETAAGRAALQQSGLAKGISPGKFISPGKQYPQPHVALPVYDKNGKEAGI
WLSSLTDSDGRLQVMGGEGRVMGNEDARFVALQGSRNGESLLAGNMGEGVRVARDNPDSGVVVRLAGDDR
PWNPGAITGGRVWADPIPVVSPEAAGTDIPLPPEVLAQRAAEEAQRQNMEKQAEQTAREVAGDGRKAGES
EDRVKEVIGDVIRGLEREKPGEEKMTLPDDPQTRKQEAAVQQVAQESLQRERLQQMERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 10942 | GenBank | WP_032716692 |
| Name | traD_MMW58_RS28855_HC6|p1 |
UniProt ID | _ |
| Length | 776 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 776 a.a. Molecular weight: 86758.82 Da Isoelectric Point: 4.9734
>WP_032716692.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Klebsiella/Raoultella group]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFIIFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLAPMR
DIIRSQPVYTINYYGRSLEYNAEQILADKYTIWCGEQLWTSFVLAAVVSLVICIVTFFVASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILLNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLSLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIQRSLDTRVDTRLSALLEAREAEGSLARALFTPDAPAPDPAEKDVKVGNTPEQSQPAE
SIESPATVKVPATAKTPAADEIGQSPETPEPAAPVTKVVTVPLIRPRSATATGTAAVATTAASTTSATTV
PAGGTEQELAQQPTEQGQDRLPAGMNDDGVIEDMDAYDAWLAGEQTQRDMQRREEVNINHSHRRDEQDDI
EIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFIIFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLAPMR
DIIRSQPVYTINYYGRSLEYNAEQILADKYTIWCGEQLWTSFVLAAVVSLVICIVTFFVASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILLNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLSLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIQRSLDTRVDTRLSALLEAREAEGSLARALFTPDAPAPDPAEKDVKVGNTPEQSQPAE
SIESPATVKVPATAKTPAADEIGQSPETPEPAAPVTKVVTVPLIRPRSATATGTAAVATTAASTTSATTV
PAGGTEQELAQQPTEQGQDRLPAGMNDDGVIEDMDAYDAWLAGEQTQRDMQRREEVNINHSHRRDEQDDI
EIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 53117..77353
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MMW58_RS28855 | 53117..55447 | - | 2331 | WP_032716692 | type IV conjugative transfer system coupling protein TraD | virb4 |
| MMW58_RS28860 | 55641..56372 | - | 732 | WP_032716670 | conjugal transfer complement resistance protein TraT | - |
| MMW58_RS28865 | 56567..57181 | - | 615 | WP_032732092 | hypothetical protein | - |
| MMW58_RS28870 | 57207..60029 | - | 2823 | WP_241723814 | conjugal transfer mating-pair stabilization protein TraG | traG |
| MMW58_RS28875 | 60029..61399 | - | 1371 | WP_032700807 | conjugal transfer pilus assembly protein TraH | traH |
| MMW58_RS28880 | 61386..61814 | - | 429 | WP_032700808 | conjugal transfer protein TrbF | - |
| MMW58_RS28885 | 61807..62373 | - | 567 | WP_064361004 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| MMW58_RS28890 | 62348..62584 | - | 237 | WP_064361003 | type-F conjugative transfer system pilin chaperone TraQ | - |
| MMW58_RS28895 | 62595..63347 | - | 753 | WP_241723815 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| MMW58_RS28900 | 63370..63696 | - | 327 | WP_064361001 | hypothetical protein | - |
| MMW58_RS28905 | 63709..63969 | - | 261 | WP_227629323 | conjugal transfer protein TrbE | - |
| MMW58_RS28910 | 63926..64306 | - | 381 | WP_064360999 | hypothetical protein | - |
| MMW58_RS28915 | 64323..66278 | - | 1956 | WP_064360998 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| MMW58_RS28920 | 66278..66907 | - | 630 | WP_064360997 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| MMW58_RS28925 | 66920..67879 | - | 960 | WP_233428435 | conjugal transfer pilus assembly protein TraU | traU |
| MMW58_RS28930 | 67920..68549 | - | 630 | WP_064360996 | type-F conjugative transfer system protein TraW | traW |
| MMW58_RS28935 | 68546..68938 | - | 393 | WP_064360995 | type-F conjugative transfer system protein TrbI | - |
| MMW58_RS28940 | 68938..71577 | - | 2640 | WP_064360994 | type IV secretion system protein TraC | virb4 |
| MMW58_RS28945 | 71662..72042 | - | 381 | WP_064361017 | hypothetical protein | - |
| MMW58_RS28950 | 72057..72395 | - | 339 | WP_064360993 | hypothetical protein | - |
| MMW58_RS28955 | 72392..72796 | - | 405 | WP_241723816 | hypothetical protein | - |
| MMW58_RS28960 | 72793..73014 | - | 222 | WP_064360991 | hypothetical protein | - |
| MMW58_RS28965 | 73039..73254 | - | 216 | WP_241723817 | hypothetical protein | - |
| MMW58_RS28970 | 73684..74253 | - | 570 | WP_072351833 | type IV conjugative transfer system lipoprotein TraV | traV |
| MMW58_RS28975 | 74316..75740 | - | 1425 | WP_064360990 | F-type conjugal transfer pilus assembly protein TraB | traB |
| MMW58_RS28980 | 75740..76477 | - | 738 | WP_064360989 | type-F conjugative transfer system secretin TraK | traK |
| MMW58_RS28985 | 76464..77030 | - | 567 | WP_064349707 | type IV conjugative transfer system protein TraE | traE |
| MMW58_RS28990 | 77048..77353 | - | 306 | WP_241723818 | type IV conjugative transfer system protein TraL | traL |
| MMW58_RS28995 | 77367..77735 | - | 369 | WP_064360988 | type IV conjugative transfer system pilin TraA | - |
| MMW58_RS29000 | 77810..78169 | - | 360 | WP_077266358 | TraY domain-containing protein | - |
| MMW58_RS29005 | 78250..78948 | - | 699 | WP_227629319 | conjugal transfer protein TraJ | - |
| MMW58_RS29010 | 79147..79548 | - | 402 | WP_064360986 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| MMW58_RS29015 | 79985..80461 | + | 477 | WP_064349718 | transglycosylase SLT domain-containing protein | - |
| MMW58_RS29020 | 80499..81029 | - | 531 | WP_064360985 | antirestriction protein | - |
| MMW58_RS29025 | 81806..82084 | - | 279 | WP_077265715 | helix-turn-helix transcriptional regulator | - |
Host bacterium
| ID | 15113 | GenBank | NZ_CP093277 |
| Plasmid name | HC6|p1 | Incompatibility group | IncFIB |
| Plasmid size | 167204 bp | Coordinate of oriT [Strand] | 79861..79910 [+] |
| Host baterium | Raoultella sp. HC6 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | arsH, arsR, arsB, arsC, merE, merD, merA, merC, merP, merT, merR, silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |