Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 114676
• Name: oriT_pA
• Organism: Agrobacterium sp. CGMCC 11546
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP047389 (122892..122923 [-], 32 nt)
• oriT length: 32 nt
• IRs (inverted repeats): 18..23, 27..32 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 32 nt

>oriT_pA
CCAAGGGCGCAATTATACGTCGCGATGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_GSF67_RS23905_pA

• ID: 9415
• GenBank: WP_006315302
• Name: traA_GSF67_RS23905_pA
• UniProt ID: A0AA87AZE6
• Length: 1100 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1100 a.a.
• Molecular weight: 123738.41 Da
• Isoelectric Point: 9.7851

>WP_006315302.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [unclassified Agrobacterium]
MAIAHFSASIVSRGSGRSVVLSAAYRHCAKMEFEREARTIDYTRKQRLLHEEFALPADAPKWVRSLIADR
SVSGASEAFWNKVEAFEKRADAQLARDLTIALPLELSADQNIALVRDFVEKHILAKGMVADWVFHDNPGN
PHIHLMTTLRPLSEDGFGSKKIAVMGDDGQPVRTKSGKILYELWAGSTEDFNVLRDGWFERQNHHLALGG
IDLKIDGRSYEKQGIDLEPTIHLGVGTKAIERKAEQQGVRPELERIELNDARRAENARRILKDPAIVLDL
IMREKSVFNERDVAKVLHRYVDDPAVFQQLMLRIMLNPEVLRLQRDTIDFATGEMVPARYSTQAMIRLEA
TMARQATWLSEREGRAVSGTILEGTFQRHEQLSEEQRTAIERIAGPARIAAVVGRAGAGKTTMMKAAREA
WELAGYRVVGGALAGKAAEGLEKEAGIESRTLASWELRWNRGRDVLDDKTIFVMDEAGMVASKQMAGFVD
AVVRAGAKIVLVGDPEQLQPIEAGAAFRAIVDRIGYAELETIYRQRADWMRKASLDLARGNVEKALITYQ
REGRVLGSRLKSEAVEYLIADWNRDYDQTKTTLILAHLRRDVRMLNVMAREKLIERGVVGEGHVFKTADG
VRRFDAGDQIVFLKNEGSLGVKNGMIAHIAEAQPNRIVAVVGEGDHRRHVVVEQRFYNNLDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDLQLYYGTRSFSFNGGLAKVLSRRQAKETTLDYERGQLY
REALRFSENRGLYIVQVARTLVRDRLDWTLREKAKLVELGQRLAAFAARLGITQSPNPHTMKEAAPMVAG
TKTFAGSVADTVGDKLGADPALKRQWEEVSARFAYVFADPETAFRAMNFDAVLAEKEMAKQVLRKIEVEP
ASIGPLKGKTGILATRSAREARRVAEVNIPALKRDLEQYLRMREMVTQRLQADEQALRQRVSIDIPALSP
AAHVVLERVRDAIDRNDLPSAMAYALSNRETKLEIDEFNKAVTERFGERTLLSNAAHEPSGKLYEELSKG
MKPEQKEELKQAWPVMRTAQQLSAHERTVQSLRQAEEQRLTQRQTPVRKQ

Protein domains

Predicted by InterproScan.

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (855-951)

• AAA domain (AAA_30) (381-565)

• MobA/MobL family (MobA_MobL) (17-243)

• Viral (Superfamily 1) RNA helicase (Viral_helicase1) (691-736)

Protein structure

No available structure.

T4CP

traG_GSF67_RS23920_pA

• ID: 10939
• GenBank: WP_006315299
• Name: traG_GSF67_RS23920_pA
• UniProt ID: _
• Length: 656 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 656 a.a.
• Molecular weight: 70090.21 Da
• Isoelectric Point: 9.7397

>WP_006315299.1 MULTISPECIES: Ti-type conjugative transfer system protein TraG [unclassified Agrobacterium]
MTVNRLLLGVLPAIIMLVVMFGTTGMEHRLAALGTSAQVKLFLGRAGLGLPYVAGAAIGVISLFAANGSA
NIKTAALSVLAGNCAVIVIAVVREAIRLAGIASSVPVGQSVLGYADPATMVGASAAFFSAVFALRVAIKG
NAAFAQGGPTRVGGKRAVHGDANWMSLAKAAKLFPDAGGIVVGERYRVDHDRVAATAFRADDLQSWGGGG
KSPLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGGLVVLDPSSEVAPMVVDYRRKAGRKVIVLD
PANPATGFNALDWIGRFGGTKEEDIVAVATWIMTDNARSASARDDFFRASAMQLLTALIADVCLSGHTGA
KDQTLRRVRVNLSEPEPKLRERLTRIYEQSESTFVRENVAVFVNMTPETFSGVYANAVKETHWLSYTNYA
ALVSGDSFSTDELATGVTDIFIALDLKVLEAHPGLARVVIGSFLNAIYNRNGEVAGRTLFLLDEVARLGY
LRILETARDAGRKYGISLALIFQSIGQMREAYGGRDASSKWFESASWISFAAINDPETAEYISKRCGDTT
VEVDQTNRSSGMKGSSRSRSKQLTRRPLILPHEVLRMRGDEQIVFTAGNPPLRCGRAIWFRRKDMSACVG
GNRFHKQATDGVKSYKAAPNTDNEET

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (163-638)

Protein structure

No available structure.

Host bacterium

• ID: 15111
• GenBank: NZ_CP047389
• Plasmid name: pA
• Incompatibility group: -
• Plasmid size: 302198 bp
• Coordinate of oriT [Strand]: 122892..122923 [-]
• Host baterium: Agrobacterium sp. CGMCC 11546

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: PFL_4714
• Symbiosis gene: -
• Anti-CRISPR: -