Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114675
Name   oriT_ECL411|unnamed5 in_silico
Organism   Enterobacter cloacae complex sp. ECL411
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP091491 (86110..86212 [-], 103 nt)
oriT length   103 nt
IRs (inverted repeats)      89..94, 96..101  (AAATAA..TTATTT)
 28..33, 40..45  (GCAAAA..TTTTGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 103 nt

>oriT_ECL411|unnamed5
CTCACCACCAAAAGTTCCACACCCCACGCAAAATTAAAGTTTTGCTGATTGGGTATTCAAATCATGCAGTTAGTGAAAAAGTAATGTGAAATAATTTATTTTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9414 GenBank   WP_016479978
Name   traI_L3069_RS27125_ECL411|unnamed5 insolico UniProt ID   A0A0G3B767
Length   1746 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1746 a.a.        Molecular weight: 189285.41 Da        Isoelectric Point: 6.0099

>WP_016479978.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMGERWAGKGAEALGLSGGVDQKVFTKVLEGRLPDGSDLSRTQ
DGANKHRPGYDFTFSAPKSVSVMAMLGDDKRLIEAHNRAVETAIKQIEAMASTRVMTEGRSETQLTGNLV
MALFNHDTSRDQEPQLHTHAVVANATQHGDEWRTLSSDKVGKTGFIENIYANQIAFGRLYRAALKDDVAA
MGYETETVGKHGMWELKGVPTEPYSSRSRTISEAVGDDASLKSRDVAALDTRQSKQKVDPEQRMAEWMQT
LKETGFDIKAYREAADLRVVQGNIPATTPEAIDINSSVGQAIAMLSDRRARFTYSELLATTLGQLPARSG
MVEMARDGIDAAIKNEQLIPLDKEKGLFTSNIHVLDELSVSAMTRDLQRTGQADIFPDKSVPRSRSYSDA
VSVLAQDRPPVAIISGQGGAAGQRERVAELTMMAREQGRDVQIIAADRRSRSALMQDERLSGEHITDRRG
LTEGMAFTPGSTLIVDQGEKLTLKETLTLLDGALRHNVQLLIADSGQRTGTGSALTVMKEAGVSTLAWQG
GEKTQVSVISEPDRRQRYDRLAADFARSVRAGEHSVAQVTGPREQAVLAGVVREALKAEKVLGEREVSIT
TLEPVWLDSRHQQVRDHYREGMVMERWNAEERSRERFVIDRVTGRNNSLTLRNAQGETRVTRLSELNSSW
SLYRTGTLQVAEGDRLAVLGQAQGTRLRGGDTVTVKALGEAGLVVSLPGRKADVVLPAGDSPFTAAKVVQ
GWVESPGRSVSDSATVFASLSQRELDNTTLNKLALSGRAVRLYSAQTAQKTTEKLSRQSAWSVVSEQIKD
AAGHDRLDDALAHQKAGLHTPEQQAIHLAIPVLEGNGLAFTKPQLMAAAKEFEQDSLSLQAIEKEADRQV
RSGDLLSVPVSPGNGLQLLVSRQSYNAEKSIIRHVLEGKEAVTPLMDKVPDAQLAGLTDGQRNATRMILE
SPDRFTLVQGYAGVGKTTQFRAVMSAIGTLPEREQPRVIGVAPTHRAVSEMRDAGVPETQTLAAFIHDTQ
QQLRGGERTDFSNVLFLVDESSMVGNADMAKAYSLIAGGGGRAVSSGDTDQLQSIAPGQPFRLLQKRSAI
DVAVMQEIVRQTPELKPAVYSLISRDIGSALTTIESVAPAQVPRRADAWQPDSSVMEFSREREEAIAKAV
AAGDLMPGGQPATLLEAIVKDYTGRTSEAQAQTIVITALNADRRQVNAMIHDARQGAGEVGEKEVTLPVL
TPANIRDGELRRMATWEASRDSLVLLDSTYYSIEALDGEAHLVMLKDAQGNTRSLSPAQAATEGVTLYRQ
DTITVSEGDRMRFSKSDNERGFVANSVWSVSEIKGDSVTLSDGKQTRTLTPSAEQAEQHIDLAYAVTVEG
SQGASEPFSISLQGTDGGRKRMVSFESAYVALSRMKQHAQVYTDNRDKWAAAMEKSQAKSTAHDILEPRG
DRAVANAARLTATAKALGEVPAGRAALRQAGLQPEGSMAKYISPGRKYPQPHVALPAFDRNGRKAGVWLS
ALTSGDGQLKGLAGEGRVMGSGDAAFAGLQASRNGESLLARDMEEGVRLARENPQSGVVVRLEGEARPWN
PGAITGGRVWADGLPDATGTQPAETVPPEVLAQQAREAEVQRELDKRAEEAVREMARSGDRPAGGADVAV
RDVVRDMDRIKETPASPLTLPDAPEERRRDEAVSQVVRESVQRDRLQQMERETVRDLEREKTLGGD

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(631-710)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(573-625)

TrwC relaxase (TrwC)

(10-283)

DNA helicase TraI (TraI_C)

(1430-1551)

AAA domain (AAA_30)

(967-1150)


  Protein structure



No available structure.




T4CP

ID   10937 GenBank   WP_077022305
Name   traD_L3069_RS27130_ECL411|unnamed5 insolico UniProt ID   _
Length   742 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 742 a.a.        Molecular weight: 83244.88 Da        Isoelectric Point: 5.1609

>WP_077022305.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIASMRIRMFSQIANIIVYCLVILFGIIVAITLSVKLTWQTFVNGMVYYWCQTLAPMR
DIFKSQPVYTIRYYGHELKYNAQQILQDKYIQWCADQVWTAFILAAIIAAVVCVITFFVTTWILGHQGKQ
QSEDEITGGRQLTDDPKAVAKMLRRDGLASDIKIGELPLIKDSEIQNFCLHGTVGAGKSEVIRRLMNYAR
QRGDMVIVYDRSCEFVKSYYDPATDKILNPLDARCAAWDLWRECLTQPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDKDRSYSKLVDTLLSIKIEKLRSYLKNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIENNGESFTIRDWMRGVREDGKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVIGIQSYAQLEDIYGQKPAETLFDVLNTRAFFRSPSRNIA
EFAAGEIGEKEILKASEQYSYGVDPVRDGTSVGKDMERVTLVSYSDVQTLPDLSCYVTLPGPYPAVKMAL
KYQQRPKVAPEFIPRTIDGKAEERLNAALAAREHESRSIAMLFEPDADVVAPEGGAENAGQPQQPQQPQQ
PQQPQQPQQPQQPQQPQQPQQLTPAPRAADAASVAGAAGAGGVEPELKTKAEEAEQLPPGISESGEVVDM
AAWEAWQAEQDELSPQERQRREEVNINVHRAPEKDLEPGGYI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.



ID   10938 GenBank   WP_008324134
Name   traC_L3069_RS27210_ECL411|unnamed5 insolico UniProt ID   _
Length   876 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 876 a.a.        Molecular weight: 98841.51 Da        Isoelectric Point: 5.7676

>WP_008324134.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacterales]
MSNPLDSVTRTVNSLLTALKMPDASSEANDVLGQMRFPQFSRVLPYRDYDAETGLFMNEKTMGFMLEAQP
INGANETIVSSLENLMRTKLPREVPVAVHLVSSHLVGQDIEYGLRELSWTGEKADTFNAITRAYYLRAAE
EGFPLPDHLNLPLTLRNYRVYISCCVPRKRGSKASVMEMENQVKIIRASLQGAQIATRSVDADAFIRIAG
EMINHNPDALYPTERELDPLKDLNYQCVDDTFDLKVKADYMTLGLRGNGRNSTARIMNFQLTKNPELAFL
WNMADNYSNLLNPELSVSCPFILTLVLSVEDQVKAQNEANLKFMDLEKKSKTSYAKFFPGVAQEAKEWGD
LRQRLSSGQSSLVSYFLNVTLFCRDDQETALKVEQDVLNSFRKNGFELISPRFNHMRNFLVNLPFMAGGG
LFKQLRESGVLQRAESFNVANLMPVVADSPLATSGLLAPTYRNQLAFIDLFSRGMGNTNFNMAVCGTSGA
GKTGLIQPLIRSVIDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLKFNPFANVTDDTIDEMAERLRDQL
SVMASPNGNLDEVHEGLLLKAVKATWLTKKNQARIDDVVDYLMMARDSEEYSGSPTIRSRLDEMVVLLTQ
YTRDGVYGSYFNSDEPSLKDEARMVVLELGGLEDRPSLLVAVMFSLILYIENRMYRTPRSLKKLNVIDEG
WRLLDFKNNKVGAFIEKGYRTARRHTGSYITITQNIVDFDSETASSAARAAWGNSSYKIILKQSAKEFAK
YNQLYPDQYSQLQRDMIKGFGSAKDNWFSSFMLSVEHLTSWHRLFVDPLSRAMYSSDGPDFEFLQQRRRE
GLSIHEAVWELAWKKSGPEMAALERWLHEHEGKTIS

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(289-446)

TraG P-loop domain (P-loop_TraG)

(470-765)

TraC protein (TraC_F_IV)

(38-276)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 61607..86760

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3069_RS27130 (L3069_27130) 61607..63835 - 2229 WP_077022305 type IV conjugative transfer system coupling protein TraD virb4
L3069_RS27135 (L3069_27135) 63969..64298 - 330 WP_223856629 tetratricopeptide repeat protein -
L3069_RS27140 (L3069_27140) 64306..64644 - 339 WP_032072912 tetratricopeptide repeat protein -
L3069_RS27145 (L3069_27145) 64719..65150 - 432 WP_008324118 entry exclusion protein -
L3069_RS27150 (L3069_27150) 65172..67994 - 2823 WP_008324119 conjugal transfer mating-pair stabilization protein TraG traG
L3069_RS27155 (L3069_27155) 67994..69364 - 1371 WP_016247527 conjugal transfer pilus assembly protein TraH traH
L3069_RS27160 (L3069_27160) 69351..69779 - 429 WP_008324122 hypothetical protein -
L3069_RS27165 (L3069_27165) 69772..70326 - 555 WP_008324125 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
L3069_RS27170 (L3069_27170) 70316..70564 - 249 WP_016479982 type-F conjugative transfer system pilin chaperone TraQ -
L3069_RS27175 (L3069_27175) 70581..71330 - 750 WP_008324127 type-F conjugative transfer system pilin assembly protein TraF traF
L3069_RS27180 (L3069_27180) 71361..71549 - 189 WP_008324128 conjugal transfer protein TrbE -
L3069_RS27185 (L3069_27185) 71581..73434 - 1854 WP_016479983 type-F conjugative transfer system mating-pair stabilization protein TraN traN
L3069_RS27190 (L3069_27190) 73431..74060 - 630 WP_008324130 type-F conjugative transfer system pilin assembly protein TrbC trbC
L3069_RS27195 (L3069_27195) 74077..75063 - 987 WP_042934586 conjugal transfer pilus assembly protein TraU traU
L3069_RS27200 (L3069_27200) 75066..75713 - 648 WP_008324132 type-F conjugative transfer system protein TraW traW
L3069_RS27205 (L3069_27205) 75713..76060 - 348 WP_008324133 type-F conjugative transfer system protein TrbI -
L3069_RS27210 (L3069_27210) 76057..78687 - 2631 WP_008324134 type IV secretion system protein TraC virb4
L3069_RS27215 (L3069_27215) 78680..79246 - 567 WP_008324135 conjugal transfer pilus-stabilizing protein TraP -
L3069_RS27220 (L3069_27220) 79236..79637 - 402 WP_016479985 hypothetical protein -
L3069_RS27225 (L3069_27225) 79621..79806 - 186 WP_008324139 hypothetical protein -
L3069_RS27230 (L3069_27230) 79810..80034 - 225 WP_008324141 TraR/DksA C4-type zinc finger protein -
L3069_RS27235 (L3069_27235) 80154..80693 - 540 WP_016247533 type IV conjugative transfer system lipoprotein TraV traV
L3069_RS27240 (L3069_27240) 80715..82109 - 1395 WP_016479986 F-type conjugal transfer pilus assembly protein TraB traB
L3069_RS27245 (L3069_27245) 82096..82839 - 744 WP_008324157 type-F conjugative transfer system secretin TraK traK
L3069_RS27250 (L3069_27250) 82826..83392 - 567 WP_016247535 type IV conjugative transfer system protein TraE traE
L3069_RS27255 (L3069_27255) 83407..83712 - 306 WP_016479987 type IV conjugative transfer system protein TraL traL
L3069_RS27260 (L3069_27260) 83863..84213 - 351 WP_016247536 type IV conjugative transfer system pilin TraA -
L3069_RS27265 (L3069_27265) 84271..84495 - 225 WP_008324165 TraY domain-containing protein -
L3069_RS27270 (L3069_27270) 84582..85271 - 690 WP_008324166 hypothetical protein -
L3069_RS27275 (L3069_27275) 85460..85852 - 393 WP_008324167 conjugal transfer relaxosome DNA-binding protein TraM -
L3069_RS27280 (L3069_27280) 86284..86760 + 477 WP_008324168 transglycosylase SLT domain-containing protein virB1
L3069_RS27285 (L3069_27285) 86807..87337 - 531 WP_008324170 antirestriction protein -
L3069_RS27290 (L3069_27290) 87964..88794 - 831 WP_008324171 N-6 DNA methylase -
L3069_RS27295 (L3069_27295) 88837..89100 - 264 WP_008324174 hypothetical protein -
L3069_RS27300 (L3069_27300) 89186..89530 - 345 WP_008324177 hypothetical protein -
L3069_RS27305 (L3069_27305) 89577..89864 - 288 WP_008324178 hypothetical protein -
L3069_RS27310 (L3069_27310) 89918..90292 - 375 WP_008324180 hypothetical protein -
L3069_RS27315 (L3069_27315) 90919..91278 - 360 WP_015493069 hypothetical protein -
L3069_RS27320 (L3069_27320) 91340..91663 - 324 WP_008324183 hypothetical protein -

Host bacterium

ID   15110 GenBank   NZ_CP091491
Plasmid name   ECL411|unnamed5 Incompatibility group   IncFIB
Plasmid size   99483 bp Coordinate of oriT [Strand]   86110..86212 [-]
Host baterium   Enterobacter cloacae complex sp. ECL411

Cargo genes

Drug resistance gene   blaNDM-1
Virulence gene   htpB
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -