Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114655
Name   oriT_MGH 3|unnamed1 in_silico
Organism   Enterobacter sp. MGH 3
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP072954 (186095..186197 [-], 103 nt)
oriT length   103 nt
IRs (inverted repeats)      89..94, 96..101  (AAATAA..TTATTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 103 nt

>oriT_MGH 3|unnamed1
CTCACCACCAAAAGCTCCACACCCCACGCAAAATTAAAGTTTTTCTGATTGGGTATTCAAATCATGCAGTTAGTGAAAAAGTAATGTGAAATAATTTATTTTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9400 GenBank   WP_022652257
Name   traI_IBG19_RS24800_MGH 3|unnamed1 insolico UniProt ID   A0A3S5I3M3
Length   1746 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1746 a.a.        Molecular weight: 189292.49 Da        Isoelectric Point: 6.1121

>WP_022652257.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacterales]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMGERWAGKGAEALGLSGGVDQKVFTKVLEGRLPDGSDLSRTQ
DGANKHRPGYDFTFSAPKSVSVMAMLGGDKRLIEAHNRAVETAIKQIEAMASTRVMTEGRSETQLTGNLV
MALFNHDTSRDQEPQLHTHAVVANATQHGDEWRTLSSDKVGKTGFIENIYANQIAFGRLYRAALKDDVAA
MGYETETVGKHGMWELKGVPTEPYSSRSRTISEAVGDDASLKSRDVAALDTRQSKQKVDPEQRMAEWMHT
LKETGFDIKAYREAADLRVVQGNIPATTPEAIDINSSVGQAIAMLSDRRARFTYSELLATTLGQLPARSG
MVEMARDGIDAAIKNEQLIPLDKEKGLFTSNIHVLDELSVSAMTRDLQRTGQADIFPDKSVPRSRSYSDA
VSVLAQDRPPVAIISGQGGAAGQRERVAELTMMAREQGRDVQIIAADRRSRSALMQDERLSGEHITDRRG
LTEGMAFTPGSTLIVDQGEKLTLKETLTLLDGALRHNVQLLIADSGQRTGTGSALTVMKEAGVSTLAWQG
GEKTQVSVISEPDRRQRYDRLAADFARSVRAGEHSVAQVTGPREQAVLAGVVREALKAEKVLGEREVSIT
TLEPVWLDSRHQQVRDHYREGMVMERWNAEERSRERFVIDRVTGRNNSLTLRNAQGETRVTRLSELNSSW
SLYRTGTLQVAEGDRLAVLGQAQGTRLRGGDTVTVKALGEAGLVVSLPGRKADVVLPAGDSPFTAAKVVQ
GWVESPGRSVSDSATVFASLSQRELDNTTLNKLALSGRAVRLYSAQTAQKTTEKLSRQSAWSVVSEQIKD
AAGHDRLDDALAHQKAGLHTPEQQAIHLAIPVLEGNGLAFTKPQLMAAAKEFEQDSLSLQAIEKEADRQV
RSGDLLSVPVSPGNGLQLLVSRQSYNAEKSIIRHVLEGKEAVTPLMDKVPDAQLAGLTDGQRNATRMILE
SPDRFTLVQGYAGVGKTTQFRAVMSAIGTLPEREQPRVIGVAPTHRAVSEMRDAGVPETQTLAAFIHDTQ
QQLRGGERTDFSNVLFLVDESSMVGNADMAKAYSLIAGGGGRAVSSGDTDQLQSIAPGQPFRLLQKRSAI
DVAVMQEIVRQTPELKPAVYSLISRDIGSALTTIESVVPAQVPRRADAWQPDSSVMEFSREREEAIAKAV
AAGDLMPGGQPATLLEAIVKDYTGRTSEAQAQTIVITALNADRRQVNAMIHDARQGAGEVGEKEVTLPVL
TPANIRDGELRRMATWEASRDSLVLLDSTYYSIEALDGEAHLVMLKDAQGNTRSLSPAQAATEGVTLYRQ
DTITVSEGDRMRFSKSDNERGFVANSVWSVSEIKGDSVTLSDGKQTRTLTPSAEQAEQHIDLAYAVTVEG
SQGASEPFSISLQGTDGGRKRMVSFESAYVALSRMKQHAQVYTDNRDKWVAAMEKSQAKSTAHDILEPRG
DRAVANAARLTATAKALGEVPAGRAALRQAGLQPEGSMAKYISPGRKYPQPHVALPAFDRNGRKAGVWLS
ALTSGDGQLKGLAGEGRVMGSGDAAFAGLQASRNGESLLARDMEEGVRLARENPQSGVVVRLEGEARPWN
PGAITGGRVWADGLPDATGTQPAETVPPEVLAQQAREAEVQRELDKRAEEAVREMARSGDRPAGGADVAV
RDVVRDMDRIKETPASPLTLPDAPEERRRDEAVSQVVRESVQRDRLQQMERETVRDLEREKTLGGD

  Protein domains


Predicted by InterproScan.

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(631-710)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(573-625)

AAA domain (AAA_30)

(967-1148)

TrwC relaxase (TrwC)

(10-283)

DNA helicase TraI (TraI_C)

(1430-1551)


  Protein structure



No available structure.




T4CP

ID   10917 GenBank   WP_022652256
Name   traD_IBG19_RS24805_MGH 3|unnamed1 insolico UniProt ID   _
Length   736 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 736 a.a.        Molecular weight: 82566.18 Da        Isoelectric Point: 5.1609

>WP_022652256.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIASMRIRMFSQIANIIVYCLVILFGIIVAITLSVKLTWQTFVNGMVYYWCQTLAPMR
DIFKSQPVYTIRYYGHELKYNAQQILQDKYIQWCADQVWTAFILAAIIAAVVCVITFFVTTWILGHQGKQ
QSEDEITGGRQLTDDPKAVAKMLRRDGLASDIKIGELPLIKDSEIQNFCLHGTVGAGKSEVIRRLMNYAR
QRGDMVIVYDRSCEFVKSYYDPATDKILNPLDARCAAWDLWRECLTQPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDKDRSYSKLVDTLLSIKIEKLRSYLKNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIENNGESFTIRDWMRGVREDGKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVIGIQSYAQLEDIYGQKPAETLFDVLNTRAFFRSPSRNIA
EFAAGEIGEKEILKASEQYSYGVDPVRDGTSVGKDMERVTLVSYSDVQTLPDLSCYVTLPGPYPAVKMAL
KYQQRPKVAPEFIPRTIDGKAEERLNAALAAREHESRSIAMLFEPDADVVAPEGGAENVGQPQQPQQPQQ
PQQPQQPQQPQQPQQLTPAPRAADAASVAGAAGAGGVEPELKTKAEEAEQLPPGISESGEVVDMAAWEAW
QAEQDELSPQERQRREEVNINVHRAPEKDLEPGGYI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.



ID   10918 GenBank   WP_008324134
Name   traC_IBG19_RS24880_MGH 3|unnamed1 insolico UniProt ID   _
Length   876 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 876 a.a.        Molecular weight: 98841.51 Da        Isoelectric Point: 5.7676

>WP_008324134.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacterales]
MSNPLDSVTRTVNSLLTALKMPDASSEANDVLGQMRFPQFSRVLPYRDYDAETGLFMNEKTMGFMLEAQP
INGANETIVSSLENLMRTKLPREVPVAVHLVSSHLVGQDIEYGLRELSWTGEKADTFNAITRAYYLRAAE
EGFPLPDHLNLPLTLRNYRVYISCCVPRKRGSKASVMEMENQVKIIRASLQGAQIATRSVDADAFIRIAG
EMINHNPDALYPTERELDPLKDLNYQCVDDTFDLKVKADYMTLGLRGNGRNSTARIMNFQLTKNPELAFL
WNMADNYSNLLNPELSVSCPFILTLVLSVEDQVKAQNEANLKFMDLEKKSKTSYAKFFPGVAQEAKEWGD
LRQRLSSGQSSLVSYFLNVTLFCRDDQETALKVEQDVLNSFRKNGFELISPRFNHMRNFLVNLPFMAGGG
LFKQLRESGVLQRAESFNVANLMPVVADSPLATSGLLAPTYRNQLAFIDLFSRGMGNTNFNMAVCGTSGA
GKTGLIQPLIRSVIDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLKFNPFANVTDDTIDEMAERLRDQL
SVMASPNGNLDEVHEGLLLKAVKATWLTKKNQARIDDVVDYLMMARDSEEYSGSPTIRSRLDEMVVLLTQ
YTRDGVYGSYFNSDEPSLKDEARMVVLELGGLEDRPSLLVAVMFSLILYIENRMYRTPRSLKKLNVIDEG
WRLLDFKNNKVGAFIEKGYRTARRHTGSYITITQNIVDFDSETASSAARAAWGNSSYKIILKQSAKEFAK
YNQLYPDQYSQLQRDMIKGFGSAKDNWFSSFMLSVEHLTSWHRLFVDPLSRAMYSSDGPDFEFLQQRRRE
GLSIHEAVWELAWKKSGPEMAALERWLHEHEGKTIS

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(289-446)

TraG P-loop domain (P-loop_TraG)

(470-765)

TraC protein (TraC_F_IV)

(38-276)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 161611..186745

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IBG19_RS24805 161611..163821 - 2211 WP_022652256 type IV conjugative transfer system coupling protein TraD virb4
IBG19_RS24810 163955..164629 - 675 WP_008324909 tetratricopeptide repeat protein -
IBG19_RS24815 164704..165135 - 432 WP_008324118 entry exclusion protein -
IBG19_RS24820 165157..167979 - 2823 WP_008324119 conjugal transfer mating-pair stabilization protein TraG traG
IBG19_RS24825 167979..169349 - 1371 WP_016247527 conjugal transfer pilus assembly protein TraH traH
IBG19_RS24830 169336..169764 - 429 WP_008324122 hypothetical protein -
IBG19_RS24835 169757..170311 - 555 WP_008324125 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
IBG19_RS24840 170301..170549 - 249 WP_008324126 type-F conjugative transfer system pilin chaperone TraQ -
IBG19_RS24845 170566..171315 - 750 WP_008324127 type-F conjugative transfer system pilin assembly protein TraF traF
IBG19_RS24850 171346..171534 - 189 WP_008324128 conjugal transfer protein TrbE -
IBG19_RS24855 171566..173419 - 1854 WP_022652255 type-F conjugative transfer system mating-pair stabilization protein TraN traN
IBG19_RS24860 173416..174045 - 630 WP_008324130 type-F conjugative transfer system pilin assembly protein TrbC trbC
IBG19_RS24865 174062..175048 - 987 WP_032160671 conjugal transfer pilus assembly protein TraU traU
IBG19_RS24870 175051..175698 - 648 WP_008324132 type-F conjugative transfer system protein TraW traW
IBG19_RS24875 175698..176045 - 348 WP_008324133 type-F conjugative transfer system protein TrbI -
IBG19_RS24880 176042..178672 - 2631 WP_008324134 type IV secretion system protein TraC virb4
IBG19_RS24885 178665..179231 - 567 WP_008324135 conjugal transfer pilus-stabilizing protein TraP -
IBG19_RS24890 179221..179622 - 402 WP_008324137 hypothetical protein -
IBG19_RS24895 179606..179791 - 186 WP_022652254 hypothetical protein -
IBG19_RS24900 179795..180019 - 225 WP_008324141 TraR/DksA C4-type zinc finger protein -
IBG19_RS24905 180139..180678 - 540 WP_008324154 type IV conjugative transfer system lipoprotein TraV traV
IBG19_RS24910 180700..182094 - 1395 WP_008324156 F-type conjugal transfer pilus assembly protein TraB traB
IBG19_RS24915 182081..182824 - 744 WP_008324157 type-F conjugative transfer system secretin TraK traK
IBG19_RS24920 182811..183377 - 567 WP_008324158 type IV conjugative transfer system protein TraE traE
IBG19_RS24925 183392..183697 - 306 WP_008324160 type IV conjugative transfer system protein TraL traL
IBG19_RS24930 183848..184198 - 351 WP_008324162 type IV conjugative transfer system pilin TraA -
IBG19_RS24935 184256..184480 - 225 WP_008324165 TraY domain-containing protein -
IBG19_RS24940 184567..185256 - 690 WP_008324166 hypothetical protein -
IBG19_RS24945 185445..185837 - 393 WP_008324167 conjugal transfer relaxosome DNA-binding protein TraM -
IBG19_RS24950 186269..186745 + 477 WP_008324168 transglycosylase SLT domain-containing protein virB1
IBG19_RS24955 186792..187322 - 531 WP_008324170 antirestriction protein -
IBG19_RS24960 187949..188779 - 831 WP_022652253 N-6 DNA methylase -
IBG19_RS24965 188822..189085 - 264 WP_008324174 hypothetical protein -
IBG19_RS24970 189171..189515 - 345 WP_022652252 hypothetical protein -
IBG19_RS24975 189562..189849 - 288 WP_008324178 hypothetical protein -
IBG19_RS24980 189903..190277 - 375 WP_008324180 hypothetical protein -
IBG19_RS24985 190998..191264 - 267 WP_022652251 hypothetical protein -
IBG19_RS24990 191326..191649 - 324 WP_008324183 hypothetical protein -

Host bacterium

ID   15090 GenBank   NZ_CP072954
Plasmid name   MGH 3|unnamed1 Incompatibility group   IncFIB
Plasmid size   241263 bp Coordinate of oriT [Strand]   186095..186197 [-]
Host baterium   Enterobacter sp. MGH 3

Cargo genes

Drug resistance gene   tet(D), sul1, qacE, ere(A), aac(6')-IIc, sul2, blaTEM-1A, aph(3')-Ia
Virulence gene   -
Metal resistance gene   pbrA, merE, merD, merA, merC, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -