Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114623
Name   oriT_pMH16-367M_1 in_silico
Organism   Morganella morganii strain MH16-367M
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_AP018565 (43043..43143 [-], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      80..85, 91..96  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_pMH16-367M_1
TATTTATTTTTTTATTTTTTAAATCAGTGTGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9376 GenBank   WP_058683159
Name   mobF_DB681_RS00265_pMH16-367M_1 insolico UniProt ID   _
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120119.40 Da        Isoelectric Point: 6.6313

>WP_058683159.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
ISGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPAEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

TrwC relaxase (TrwC)

(16-287)

AAA domain (AAA_30)

(477-653)


  Protein structure



No available structure.




Auxiliary protein

ID   5502 GenBank   WP_001532083
Name   WP_001532083_pMH16-367M_1 insolico UniProt ID   A8R734
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001532083.1 MULTISPECIES: hypothetical protein [Enterobacterales]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCLEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A8R734


T4CP

ID   10883 GenBank   WP_000342688
Name   t4cp2_DB681_RS00260_pMH16-367M_1 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 55245..65570

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DB681_RS00285 51356..51748 + 393 WP_001351729 cysteine hydrolase family protein -
DB681_RS00290 51886..52770 + 885 WP_000058717 DMT family transporter -
DB681_RS00295 52802..54001 - 1200 WP_000804064 tetracycline efflux MFS transporter Tet(A) -
DB681_RS00300 54107..54757 + 651 WP_001493764 tetracycline resistance transcriptional repressor TetR(A) -
DB681_RS00305 54789..55001 - 213 WP_072024976 relaxase -
DB681_RS00310 55000..55245 - 246 Protein_62 phospholipase D-like domain-containing protein -
DB681_RS00315 55245..56240 - 996 WP_000128596 ATPase, T2SS/T4P/T4SS family virB11
DB681_RS00320 56282..57442 - 1161 WP_000101711 type IV secretion system protein VirB10 virB10
DB681_RS00325 57442..58326 - 885 WP_000735067 TrbG/VirB9 family P-type conjugative transfer protein virB9
DB681_RS00330 58337..59035 - 699 WP_000646594 virB8 family protein virB8
DB681_RS21075 59025..59183 - 159 WP_012561180 hypothetical protein -
DB681_RS00335 59254..60294 - 1041 WP_000886022 type IV secretion system protein virB6
DB681_RS00340 60310..60537 - 228 WP_000734973 IncN-type entry exclusion lipoprotein EexN -
DB681_RS00345 60545..61258 - 714 WP_000749362 type IV secretion system protein virB5
DB681_RS00350 61276..63744 - 2469 WP_225303560 VirB4 family type IV secretion/conjugal transfer ATPase virb4
DB681_RS00355 63876..64193 - 318 WP_000496058 VirB3 family type IV secretion system protein virB3
DB681_RS00360 64243..64536 - 294 WP_000209137 TrbC/VirB2 family protein virB2
DB681_RS00365 64546..64827 - 282 WP_000440698 transcriptional repressor KorA -
DB681_RS00370 64836..65570 - 735 WP_000033783 lytic transglycosylase domain-containing protein virB1
DB681_RS00375 65679..65984 + 306 WP_000960954 H-NS family nucleoid-associated regulatory protein -
DB681_RS00380 66000..66377 + 378 WP_000504251 hypothetical protein -
DB681_RS00385 66340..66654 + 315 WP_000734776 TrbM/KikA/MpfK family conjugal transfer protein -
DB681_RS00390 66690..67001 + 312 WP_001452736 hypothetical protein -
DB681_RS00395 67057..67698 + 642 WP_001694850 restriction endonuclease -
DB681_RS00400 67703..67900 + 198 WP_000844102 hypothetical protein -
DB681_RS00405 67920..68192 - 273 Protein_82 IS1 family transposase -
DB681_RS00410 68286..69719 + 1434 WP_001288435 DNA cytosine methyltransferase -

Host bacterium

ID   15058 GenBank   NZ_AP018565
Plasmid name   pMH16-367M_1 Incompatibility group   IncN
Plasmid size   73129 bp Coordinate of oriT [Strand]   43043..43143 [-]
Host baterium   Morganella morganii strain MH16-367M

Cargo genes

Drug resistance gene   qepA1, rmtB, blaTEM-1B, blaNDM-1, tet(A)
Virulence gene   htpB
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -