Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114589
Name   oriT_pEC-IMP in_silico
Organism   Enterobacter cloacae
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NC_012555 (211266..211520 [-], 255 nt)
oriT length   255 nt
IRs (inverted repeats)      153..158, 160..165  (AAAAGT..ACTTTT)
 122..130, 135..143  (TTAAGGCTT..AAGCCTTAA)
 49..55, 60..66  (GAATTTT..AAAATTC)
Location of nic site      78..79
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 255 nt

>oriT_pEC-IMP
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9350 GenBank   WP_012477394
Name   mobH_HS677_RS01115_pEC-IMP insolico UniProt ID   A0A2Z2E7B6
Length   1050 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1050 a.a.        Molecular weight: 118067.47 Da        Isoelectric Point: 4.6347

>WP_012477394.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure


Source ID Structure
AlphaFold DB A0A2Z2E7B6


T4CP

ID   10850 GenBank   WP_001284073
Name   traD_HS677_RS01120_pEC-IMP insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78123.91 Da        Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

AAA-like domain (AAA_10)

(199-249)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 2429..11636

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS677_RS00005 (pEC-IMP_001) 1..876 + 876 WP_000594611 RepB family plasmid replication initiator protein -
HS677_RS00010 (pEC-IMP_002) 2025..2378 + 354 WP_000423602 hypothetical protein -
HS677_RS00015 (pEC-IMP_003) 2429..2746 + 318 WP_000043358 type IV conjugative transfer system protein TraL traL
HS677_RS00020 (pEC-IMP_004) 2758..3546 + 789 WP_000783153 TraE/TraK family type IV conjugative transfer system protein traE
HS677_RS00025 (pEC-IMP_005) 3546..4817 + 1272 WP_000592092 type-F conjugative transfer system secretin TraK traK
HS677_RS00030 (pEC-IMP_006) 4819..5280 + 462 WP_000521242 plasmid transfer protein HtdO -
HS677_RS00035 (pEC-IMP_007) 5270..6625 + 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
HS677_RS00040 (pEC-IMP_008) 6633..7115 + 483 WP_000377632 hypothetical protein -
HS677_RS00045 (pEC-IMP_009) 7234..7986 + 753 WP_182911723 protein-disulfide isomerase HtdT -
HS677_RS00050 (pEC-IMP_010) 7996..8946 + 951 WP_001022588 IncHI-type conjugal transfer lipoprotein TrhV traV
HS677_RS00055 (pEC-IMP_011) 8955..11636 + 2682 WP_000387412 TraC family protein virb4
HS677_RS01730 12733..12888 + 156 WP_001371891 hypothetical protein -
HS677_RS00060 14185..14346 + 162 WP_001371949 hypothetical protein -

Host bacterium

ID   15024 GenBank   NC_012555
Plasmid name   pEC-IMP Incompatibility group   IncHI2A
Plasmid size   318782 bp Coordinate of oriT [Strand]   211266..211520 [-]
Host baterium   Enterobacter cloacae

Cargo genes

Drug resistance gene   blaTEM-1B, tet(D), blaIMP-8, catB3, qacE, sul1, dfrA19, aph(3'')-Ib, aph(6)-Id, mcr-9, aph(3')-Ia, catA2, blaSHV-12, aac(6')-IIc, ere(A), sul2
Virulence gene   -
Metal resistance gene   terW, terZ, terA, terB, terC, terD, terE, pbrA, merE, merD, merA, merC, merP, merT, merR, pcoS, pcoE, ncrC, rcnR/yohL, arsC, arsB, arsR, arsH
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -