Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114583
Name   oriT_T29|unnamed2 in_silico
Organism   Agrobacterium sp. T29
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP040642 (16473..16501 [+], 29 nt)
oriT length   29 nt
IRs (inverted repeats)      14..19, 24..29  (CGTCGC..GCGACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 29 nt

>oriT_T29|unnamed2
AGGGCGCAATATACGTCGCTCGCGCGACG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9345 GenBank   WP_142781652
Name   traA_FGF54_RS25170_T29|unnamed2 insolico UniProt ID   _
Length   1547 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1547 a.a.        Molecular weight: 171184.93 Da        Isoelectric Point: 6.6952

>WP_142781652.1 Ti-type conjugative transfer relaxase TraA [Agrobacterium sp. T29]
MAIMFVRAQVIGRGAGRSIVSAAAYRHRARMIDEQVGTSFSYRGSASELVHEELALPDQIPTWLKSAIDG
KSVAGVSQALWNAVDAFETRADAQLARELIIALPGELTRAENIALVREFVADNFTSKGMIADWVFHDKDG
NPHIHLMTTLRPLTEEGFGPKKVPVLGEDGEPLRVVTPDRPNGKIVYTLWAGDKETMKAWKIAWADTANR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALARKGIEMYFAPGDLARRQVMADRLLAEPELLLKQ
LANERSTFDEKDIAKALHRYVDDPVDFANIRARLMASDDLVLLKSQQVDAETGKAAEPAVFTTREILRIE
YDMAQSASVLSKRRGFAVAAEAVAAAIGNVETQNPQKPICLDTEQVDAIRHVTGDKGIAAVVGLAGAGKS
TLLAAARVAWESEGRRVIGAALAGKAAEGLEDSSGIRSRTLASLELAWAGGRDLLERGNVLVIDEAGMIS
SQQMGRVLKVAEDAGAKVVLVGDAMQLQPIQAGAAFRAISDRIGFAELAGVRRQRQEWAREASRLFARGN
VRDGLDAYTQQGRIAEAETRAEIVDRIVADWTDAKRNLIQNTTDEGDAPPLHGDELLVLAHTNEDVKRLN
ESLRKVMMEEGALTGAREFLTARGVREFAAGDRIIFLENARFLEPRARHLGPQYVKNGMLGTVVSTGDKR
GGPLLSVRLDNGRDVVISEDSYRNIDHGYAATIHKSQGATVDRTFVLATGMMDQHLTYVSMTRHRDRADL
YAAREDFEPKLEWERKPRADHAAGVTGELVETGQAKFRPQDEDAKESPYADVKTDDGVVHRLWGVSLPKA
LEDGAVSQGDTVTLRKDGVETVKVEVTVVDEQTREKRTEEREVDRNVWTAELKETAKAREQRIEQESHRP
DLFSQLVERLGRSGAKSTTLDFESEEGYRIHAEDFARRRGIDTFAGITAGMEEGVSKWLAWIADKRAQVA
KLWERASVALGFVIERERHVSYNEGRTETLATEIPTTAKYLIPPTTRFARSVDKDARRAQLASERWKERD
AILRLVLEKIYRDPDSALARLNALASDAANEPRKLADDLAAAPDWLGRLRGSDLMVDGRADHDERNGATT
ALLELLPLARAHATEFRRQAERFGIREEQRRAHMSLSVPMLSKPAMARLVEIEKIREHGGDDAYKIAFAY
AVEDRRLVPEVEAVNDALTARFGWSAFTTKADTVAERNVTERMPEDIAPERREKLFRLFAVVRRFAEEQH
LAEKQDRSKIVAGSSVEVGKETLPMLPMLAAVTEFKMRVEEEARNRAVAVAHYRHHRAALAVTAARIWRD
PAGAVAKIEELVRQGFAGERIAAAISNDPAAYGPLRGSDRIMDRLLAAGRERKEALQTVPEAAACVCPLA
TSWATALDAETQVITEDRQRMAIAIPGLSQAAEEALRTLVADMNKQKKKEDRGKKTRRDFTAGSLPADIK
SEFTAVSRALDERFGSNAILRGDKDAVNRVPPAQRNAFEAMRERLQVLQLTVRIERSQEIVAERQGRAID
QARGLIR

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(17-249)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(1048-1122)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(1309-1393)

AAA domain (AAA_30)

(391-578)


  Protein structure



No available structure.




T4CP

ID   10842 GenBank   WP_142781649
Name   traG_FGF54_RS25155_T29|unnamed2 insolico UniProt ID   _
Length   639 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 639 a.a.        Molecular weight: 70503.40 Da        Isoelectric Point: 9.2606

>WP_142781649.1 Ti-type conjugative transfer system protein TraG [Agrobacterium sp. T29]
MALKGKLHPSLLLVFVPIVVTAITVHIVGWRWTGLATGISGKTAYWFLRAAPLPALLLGPLAGLLTVWAL
PMHRRRPIAVVSLLCFLGIVGFYALREYGRLAPSVEHGVVSWDYALSYLDMVAVVGALCGFMAVAASARI
STVVPEQVKRARRATFGDADWLSMSAAGKLFPSDGEIVVGERYRVDKEIVHELPFDPNDRSTWGQGGKAP
LLTYNQDFDSTHVLFFAGSGGYKTTSNVVPTALRYTGPLICLDPSTEVVPMVIEHRSRVLNRDVMVLDPT
NPIMGFNVLDGIETSRQKEEDIVGVAHMLLSESIRFESSTGSYFQSQAHNLLTGLLAHVMLSPDYTGRRN
LRSLRQIVSEPEPSVLAMLRDIQENSGSTFIRETLGVFTNMTEQTFSGVYSTASKDTQWLSLDSYAALVC
GNAFKSSDIVTGKKDVFLNIPASILRSYPGIGRVIIGALINAMTQADGAFKRRALFMLDEVDLLGYMRVL
EEARDRGRKYGISMMLMYQSVGQLERHFGKDGATSWIDGCAFASYAAIKALDTARNVSAQCGEMTVEVKG
SSRNIGWDTKNSASRKSENVNFQRRPLIMPHEITQSMRKDEQIIIVQGHSPIRCGRAIYFRRKEMDAAAR
VNRFVKLGP

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(161-634)

  Protein structure



No available structure.



ID   10843 GenBank   WP_142781931
Name   tcpA_FGF54_RS26885_T29|unnamed2 insolico UniProt ID   _
Length   1750 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 1750 a.a.        Molecular weight: 194623.98 Da        Isoelectric Point: 5.4415

>WP_142781931.1 FtsK/SpoIIIE domain-containing protein [Agrobacterium sp. T29]
MDIHELIGKVACSILKEELGDASGEAGTARFLLDGLSIPQTLAVSRSVLADPFLSEAVEIKLPRGLFEGH
ALPDAILTDRNATFYRSADCDKAAFLITNASADEGQAEDMSLHEVTPIGAAQLIERLSAWVLAASAGLAL
TEDAKAWWERSLAGLSQVGSVALERFARYVVATREAVIDEGYPIIEALGYALPALQLPRDPAAFAGIKDK
SRRHASVWKREFIGLRRKRHPYLLKQNPNQIVISETELRDAFEKARDVIPENIHPVVALFIESRPAWNSS
SEALANCLWEHVKPLFEGLAREKTNLGQDTQRFYAEGPEDLLSKEDDEYLELLARRKTTGSPDPEDIEFY
ESHRDEIREDRKLKSSWDKFIYGRPLETEDFLTGLAMVMDTLNARAMVGVERKLTIRCDSLTKRDLRSLN
TEAGLFFSLRYAGLQKLLGAGVQIEFGSLMDYPAVLRGWQDAKDKSAVNRSTAKAAMQLRFQLELETTDF
EGGTSTASAQLIWKYRPDVISSQLTDDWDRLCEHAFVALRCGREPGMAGRRPGSIDLSDVKTLVPAYDRD
RGSLIPTYRRERDLRLLWRANLKTALENNLIDAAASTKISTGFDAFCERYQTAIRSFRVDGASNLLCRDQ
ATSYAELLDLVRLLAPGDRNKDLLLRPLLELGQAPVGDGAAAAIVAPWHPLRLAAIWRKAHLVREVVRKV
IDLPAGLEGDTKLFFRDLSEDMRHVFYPEVVVSWSGHRPELLSLADHQGDYSLHERPVLDGAGGGETNDD
ARAGTNTLLDLTDRYLKLHPHERANMSLVLYNCDSARLPQQLVDGLGDLNDEEDMRCQVMLRHTDGTRLR
DIYRSILTSTSGSAEVLAPSEITQDFMARLRISVIADQAPPPDPRDGRPYDIVFSQDVISRHASVEWYRE
TADPADIATLLPARWSRRRPGAMDDLKSSVYLCSPVQSREGWAFLSAITAFLKEEENLQDGKRLLPVRQL
DFRDDRTARIFEETHDLGAWVVNYDELLDRRQLQNQSVRVIRYKQSATQGRNVVISSRAPLTLLRAMVRR
RLEELQLDLSPSQIDALADRFIGDANDISGDIVLRAAKRGEAAGELIGVVLSRFLARQSLDENRLVGWYF
LDDYASWLGAREETQADILALSPALDADGHLSLRIIVTEAKYVDAVSFASKRKESEKQLRDTMRRIIDAM
AEPDRLDRESWLSRLGDLLLDGIQIPAASNVPLVEWRRRVREGAARIEVVGLSHVFVPTALDGTNITDFS
EVSGAPASRQMIFGRAELKTLITAYIENSKVDRFLTSIDPIYGRVPAPHMPGATVDATAKAAPSPVQTKS
DPVIGDGGHHAEPPTPADEPPIPKSLEPELAGAPSSVRTVDEIVARHLDTSPRQTEADDEWLANTAMMAR
SALQQLGLQAKLIDQKLTPNAAILRFSGSANLTVEQVLKRRSELLTTHRLDIISVRPEPGAIAIYVARNQ
RQVVQIQSLWSRWKPVVSPTGNQDLVVGVREDDNELFVLSPSQRHAPHTLIAGSTGSGKSVLMQSIILGI
AATNDPCHAKIVLIDPKQGVDYFAFDSLPHLEGGIIDQQENAVDQLEHMVLEMDRRYRLFKEARVSNITA
YNAKATPGEVLPTYWIIHDEFAEWMLTDDYRAAVTSTVGRLGVKARAAGIHLIFAAQRPEANVMPMQLRS
QLGNRLILRVDSEGTSEIALGEKGAERLLGKGHMIVRAEGEQGLIYAQVPFASEHFITDVVNSIMLQSVS

  Protein domains


Predicted by InterproScan.

FtsK/SpoIIIE family (FtsK_SpoIIIE)

(1513-1616)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 420564..430147

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGF54_RS26950 416369..417319 + 951 WP_246667058 phytanoyl-CoA dioxygenase family protein -
FGF54_RS26955 417587..418216 - 630 WP_142781943 RES family NAD+ phosphorylase -
FGF54_RS26960 418213..418998 - 786 WP_142781944 hypothetical protein -
FGF54_RS26965 419573..419923 - 351 WP_080855390 MarR family transcriptional regulator -
FGF54_RS26970 420022..420561 + 540 WP_142781945 hypothetical protein -
FGF54_RS26975 420564..421229 + 666 WP_142781946 lytic transglycosylase domain-containing protein virB1
FGF54_RS26980 421226..421525 + 300 WP_142781947 TrbC/VirB2 family protein virB2
FGF54_RS26985 421532..421870 + 339 WP_142781948 type IV secretion system protein VirB3 virB3
FGF54_RS26990 421863..424229 + 2367 WP_142781949 VirB4 family type IV secretion/conjugal transfer ATPase virb4
FGF54_RS26995 424226..424927 + 702 WP_142781950 P-type DNA transfer protein VirB5 virB5
FGF54_RS27000 424924..425157 + 234 WP_142781951 EexN family lipoprotein -
FGF54_RS27005 425161..426093 + 933 WP_142781952 type IV secretion system protein virB6
FGF54_RS27010 426133..426414 + 282 WP_142781953 hypothetical protein -
FGF54_RS27015 426416..427087 + 672 WP_142781954 virB8 family protein virB8
FGF54_RS27020 427084..427941 + 858 WP_142781955 P-type conjugative transfer protein VirB9 virB9
FGF54_RS27025 427950..429125 + 1176 WP_142781956 type IV secretion system protein VirB10 virB10
FGF54_RS27030 429134..430147 + 1014 WP_142781957 P-type DNA transfer ATPase VirB11 virB11
FGF54_RS27035 430375..431073 + 699 WP_246667059 hypothetical protein -
FGF54_RS27040 431070..431657 + 588 WP_142781958 RES family NAD+ phosphorylase -
FGF54_RS27045 432192..432452 + 261 WP_142781959 hypothetical protein -

Host bacterium

ID   15018 GenBank   NZ_CP040642
Plasmid name   T29|unnamed2 Incompatibility group   -
Plasmid size   451191 bp Coordinate of oriT [Strand]   16473..16501 [+]
Host baterium   Agrobacterium sp. T29

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -