Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 114522
• Name: oriT_pRCEID2.9
• Organism: Lacticaseibacillus casei
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NC_017466 (206..242 [+], 37 nt)
• oriT length: 37 nt
• IRs (inverted repeats): 14..21, 28..35 (AAAGTATA..TATACTTT) 1..7, 12..18 (ACTTTGT..ACAAAGT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 37 nt

>oriT_pRCEID2.9
ACTTTGTGATAACAAAGTATACTGGATTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_HS715_RS00010_pRCEID2.9

• ID: 9303
• GenBank: WP_014562755
• Name: mobV_HS715_RS00010_pRCEID2.9
• UniProt ID: E3VQ22
• Length: 436 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 436 a.a.
• Molecular weight: 50892.23 Da
• Isoelectric Point: 9.3042

>WP_014562755.1 MULTISPECIES: MobV family relaxase [Lacticaseibacillus]
MSQLAAHMQKFKIGNLGGLQRHDERTLQRHSNPDIDVSKSSDNFSVLPLERLDPKRSLHKQVQEIIADER
VSNRAVRKDAVVLTEWVISSDSTFFEGLSRDETKQFFTDAYQWFANHFGADHIPYATVHMDETTPHMHMG
VIPLTNGRLSAKAIFNREALRFIQADLPQKLNEKGWNIERGERGSKRKHLSVERYKDVAESLKQGEPLFD
VSKLQHERVPIVNMFNFVKGKEATQNYILTPEDYQRLTERVQGFSDLGHAVKDVNELKTALSARESRIRQ
REKELDKREDAISKNENLIETYDYWYDQAYEQSDKVTQLQDQIKTLKAENKQWQQRYYKLVDAVHDMAKT
IGTLIADYPAWEQKLNEFGQRVIRGTRAFAKNTLNKVQAYGAAEDIDTTVEISKSIEPDMYPERKQKRRQ
QLQQQRKRQQQQGLER

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (4-197)

Protein structure

• AlphaFold DB: E3VQ22

Host bacterium

• ID: 14957
• GenBank: NC_017466
• Plasmid name: pRCEID2.9
• Incompatibility group: -
• Plasmid size: 2952 bp
• Coordinate of oriT [Strand]: 206..242 [+]
• Host baterium: Lacticaseibacillus casei

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -