Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114514
Name   oriT_NMI2990_17|p1 in_silico
Organism   Klebsiella oxytoca strain NMI2990_17
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP129617 (171547..171652 [+], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      30..35, 41..46  (CCGCCG..CGGCGG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 106 nt

>oriT_NMI2990_17|p1
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9298 GenBank   WP_050868819
Name   traI_QTO83_RS30090_NMI2990_17|p1 insolico UniProt ID   A0A142BMF1
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75234.98 Da        Isoelectric Point: 10.0285

>WP_050868819.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A142BMF1


T4CP

ID   10792 GenBank   WP_011091092
Name   t4cp2_QTO83_RS29805_NMI2990_17|p1 insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79491.81 Da        Isoelectric Point: 4.8270

>WP_011091092.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 174448..193551

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QTO83_RS30070 (QTO83_030065) 169532..169843 + 312 WP_011091065 hypothetical protein -
QTO83_RS30075 (QTO83_030070) 169976..170512 + 537 WP_011091066 hypothetical protein -
QTO83_RS30080 (QTO83_030075) 171013..171378 - 366 WP_011091067 hypothetical protein -
QTO83_RS30085 (QTO83_030080) 171444..171971 + 528 WP_225986328 plasmid mobilization protein MobA -
QTO83_RS30090 (QTO83_030085) 171958..173937 + 1980 WP_050868819 TraI/MobA(P) family conjugative relaxase -
QTO83_RS30095 (QTO83_030090) 173951..174451 + 501 WP_004187320 DotD/TraH family lipoprotein -
QTO83_RS30100 (QTO83_030095) 174448..175227 + 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
QTO83_RS30105 (QTO83_030100) 175238..176401 + 1164 WP_004187313 plasmid transfer ATPase TraJ virB11
QTO83_RS30110 (QTO83_030105) 176391..176651 + 261 WP_004187310 IcmT/TraK family protein traK
QTO83_RS30115 (QTO83_030110) 176676..179888 + 3213 WP_289192538 LPD7 domain-containing protein -
QTO83_RS30120 (QTO83_030115) 179854..180366 + 513 WP_011091071 hypothetical protein traL
QTO83_RS30125 (QTO83_030120) 180367..180579 - 213 WP_305953721 Hha/YmoA family nucleoid-associated regulatory protein -
QTO83_RS30130 (QTO83_030125) 180551..180961 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
QTO83_RS30135 (QTO83_030130) 181029..181742 + 714 WP_109914255 DotI/IcmL family type IV secretion protein traM
QTO83_RS30140 (QTO83_030135) 181751..182902 + 1152 WP_011091074 DotH/IcmK family type IV secretion protein traN
QTO83_RS30145 (QTO83_030140) 182914..184263 + 1350 WP_011091075 conjugal transfer protein TraO traO
QTO83_RS30150 (QTO83_030145) 184275..184979 + 705 WP_011091076 conjugal transfer protein TraP traP
QTO83_RS30155 (QTO83_030150) 185003..185533 + 531 WP_011091077 conjugal transfer protein TraQ traQ
QTO83_RS30160 (QTO83_030155) 185550..185939 + 390 WP_011091078 DUF6750 family protein traR
QTO83_RS30165 (QTO83_030160) 185985..186479 + 495 WP_011091080 hypothetical protein -
QTO83_RS30170 (QTO83_030165) 186476..189526 + 3051 WP_011091081 hypothetical protein traU
QTO83_RS30175 (QTO83_030170) 189523..190731 + 1209 WP_011091082 conjugal transfer protein TraW traW
QTO83_RS30180 (QTO83_030175) 190728..191378 + 651 WP_011091083 hypothetical protein -
QTO83_RS30185 (QTO83_030180) 191371..193551 + 2181 WP_004187492 DotA/TraY family protein traY
QTO83_RS30190 (QTO83_030185) 193554..194207 + 654 WP_011091084 hypothetical protein -
QTO83_RS30195 (QTO83_030190) 194282..194512 + 231 WP_011091085 IncL/M type plasmid replication protein RepC -
QTO83_RS30395 194763..194813 + 51 WP_011091097 IncL/M-type plasmid replication leader peptide RepB -
QTO83_RS30200 (QTO83_030195) 194810..195865 + 1056 WP_011091086 plasmid replication initiator RepA -
QTO83_RS30205 (QTO83_030200) 196869..197729 - 861 WP_000027057 broad-spectrum class A beta-lactamase TEM-1 -

Host bacterium

ID   14949 GenBank   NZ_CP129617
Plasmid name   NMI2990_17|p1 Incompatibility group   IncFIB
Plasmid size   210264 bp Coordinate of oriT [Strand]   171547..171652 [+]
Host baterium   Klebsiella oxytoca strain NMI2990_17

Cargo genes

Drug resistance gene   tet(B), sul1, qacE, aadA5, dfrA17, qnrS2, aac(6')-Ib-cr, blaOXA-1, catB3, ARR-3, mph(A), blaCTX-M-3, blaTEM-1B, aac(3)-IId
Virulence gene   -
Metal resistance gene   merR, merT, merP, merC, merA, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -