Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114511
Name   oriT_ECL404|unnamed1 in_silico
Organism   Enterobacter cloacae complex sp. ECL404
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP091497 (177437..177691 [-], 255 nt)
oriT length   255 nt
IRs (inverted repeats)      153..158, 160..165  (AAAAGT..ACTTTT)
 122..130, 135..143  (TTAAGGCTT..AAGCCTTAA)
 49..55, 60..66  (GAATTTT..AAAATTC)
Location of nic site      78..79
Conserved sequence flanking the
  nic site    		  TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 255 nt

>oriT_ECL404|unnamed1
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9297 GenBank   WP_012477394
Name   mobH_L3071_RS23770_ECL404|unnamed1 insolico UniProt ID   A0A2Z2E7B6
Length   1050 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1050 a.a.        Molecular weight: 118067.47 Da        Isoelectric Point: 4.6347

>WP_012477394.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MMMNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPT
GIQTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGG
LLSHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWT
PSSQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYT
DGNDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGE
VYLNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFR
NGIGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDT
VETAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESE
DESAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGS
LDGMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLE
LPPPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVG
SCATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAA
ATSVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPAS
PVSGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALVLHQILSKMEVAFKRKRKNRFMVSTPNTL
YLTQSCVEKFGSQLEAQDLFNKLPQYLVNSGAVINTKCHAFNMPTLLAASDRAKVDIERIINNLKEAGNL

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(108-304)


  Protein structure


Source ID Structure
AlphaFold DB A0A2Z2E7B6


T4CP

ID   10791 GenBank   WP_001284073
Name   traD_L3071_RS23775_ECL404|unnamed1 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78123.91 Da        Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(406-609)

AAA-like domain (AAA_10)

(199-249)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 308316..317523

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L3071_RS24500 (L3071_24500) 303426..304142 - 717 WP_000838219 zinc finger-like domain-containing protein -
L3071_RS24505 (L3071_24505) 304139..304339 - 201 WP_000594930 hypothetical protein -
L3071_RS24510 (L3071_24510) 304359..305411 - 1053 WP_236901918 hypothetical protein -
L3071_RS24515 (L3071_24515) 305888..306763 + 876 WP_000594611 RepB family plasmid replication initiator protein -
L3071_RS24520 (L3071_24520) 307912..308265 + 354 WP_000423602 hypothetical protein -
L3071_RS24525 (L3071_24525) 308316..308633 + 318 WP_000043358 type IV conjugative transfer system protein TraL traL
L3071_RS24530 (L3071_24530) 308645..309433 + 789 WP_000783153 TraE/TraK family type IV conjugative transfer system protein traE
L3071_RS24535 (L3071_24535) 309433..310704 + 1272 WP_000592092 type-F conjugative transfer system secretin TraK traK
L3071_RS24540 (L3071_24540) 310706..311167 + 462 WP_000521242 plasmid transfer protein HtdO -
L3071_RS24545 (L3071_24545) 311157..312512 + 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
L3071_RS24550 (L3071_24550) 312520..313002 + 483 WP_000377632 hypothetical protein -
L3071_RS24555 (L3071_24555) 313121..313873 + 753 WP_182911723 protein-disulfide isomerase HtdT -
L3071_RS24560 (L3071_24560) 313883..314833 + 951 WP_001022588 IncHI-type conjugal transfer lipoprotein TrhV traV
L3071_RS24565 (L3071_24565) 314842..317523 + 2682 WP_000387412 TraC family protein virb4

Host bacterium

ID   14946 GenBank   NZ_CP091497
Plasmid name   ECL404|unnamed1 Incompatibility group   IncHI2
Plasmid size   319000 bp Coordinate of oriT [Strand]   177437..177691 [-]
Host baterium   Enterobacter cloacae complex sp. ECL404

Cargo genes

Drug resistance gene   blaTEM-1B, dfrA19, aph(3'')-Ib, aph(6)-Id, mcr-9, aph(3')-Ia, tet(D), catA2, blaSHV-12, sul1, blaDHA-1, qnrB4, qacE, ere(A), aac(6')-IIc
Virulence gene   -
Metal resistance gene   terW, terZ, terA, terB, terC, terD, terE, pbrA, merE, merD, merC, merP, merT, merR, pcoS, pcoE, ncrC, rcnR/yohL, arsC, arsB, arsR, arsH
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -