Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114449
Name   oriT_pPC997_2 in_silico
Organism   Legionella sp. PC997
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP059578 (43100..43132 [-], 33 nt)
oriT length   33 nt
IRs (inverted repeats)      3..8, 14..19  (ACGTTG..CAACGT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 33 nt

>oriT_pPC997_2
GCACGTTGTAACGCAACGTATAAGCGCGCACTT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9263 GenBank   WP_182393739
Name   traA_HBNCFIEN_RS17430_pPC997_2 insolico UniProt ID   _
Length   889 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 889 a.a.        Molecular weight: 101385.30 Da        Isoelectric Point: 6.6355

>WP_182393739.1 Ti-type conjugative transfer relaxase TraA [Legionella sp. PC997]
MAIYRFSAQIISRSQGRSVVAASAYRSGEELIDERTSVIHDFTNKSEVIHKEILLPESAPQWMSNRSILW
NAVELCEKRKDAQLAREVQLTLPRELSLEQNIELVREFVTNEFVARGMVADVCLHNPTGDDGLYQPHAHI
LLCLRQAHEDGFGLKERSWNDNALLEHWREEWANCENRHLALYGIDQKVDHRSYKEQGIALEPQYKIGPK
DAQARMARLADHQRIARENGQLIFERPEIALDAITRCQSTFTLQDIARFINRHTENAEQFSLVFERVKSS
PELVYLGLDKKGGQRFSTQNMLQIESSMMHQSDTLHHRLGHGVSEKTILLTQSSRTLSEEQKSALRYLTQ
TGDLKSLLGYAGTGKSYLLGAARESWEQSGYRVHGAALSGIAALNLRDSSGINSRTIASLFYRLDKGMFH
FTSRDILVVDEAGMLGSHTMERLTREIEQAGAKLVLVGDWQQLQAIEAGAAFRAIAENYQYVELNQIRRQ
STPWQVEASLDLAQGEVDKALLAYDAHDHVHHFVNTHDAKQALIEQWNDVRISSPADSQIILAYTRKDVK
ELNEMARSMKRKDGQLGENIVFNTERGERGFAVNDRVYFLKREDSLSVINGTLGTIKSIDAKAGIISVAL
DGDNSKSKPQIVQVNTNYYKHIEHGYAATVYKAQGVTVDRTYVLPTAHYDAHSTYVAMTRHRKSCDLFVS
REVFTHDKSLIYSLGRNRAKDITLDYTQMHGEFARQRGLLRDNPPALSYVSEMTFKTSEDSLESLMNQVM
GRDLQKEQQKLDEFERQCLRENPDQSLHIADSLLSRAEHQAKTLAHSFQPYSKLLSQDRLTSAQKDELIL
LMRQLPDDPQLMNAFKKEYPDFAKQAEYFIQNNEQQIQCIRIIDKELDL

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(336-522)

MobA/MobL family (MobA_MobL)

(17-213)


  Protein structure



No available structure.




T4CP

ID   10740 GenBank   WP_182393741
Name   traD_HBNCFIEN_RS17440_pPC997_2 insolico UniProt ID   _
Length   617 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 617 a.a.        Molecular weight: 70817.46 Da        Isoelectric Point: 7.6756

>WP_182393741.1 type IV conjugative transfer system coupling protein TraD [Legionella sp. PC997]
MSKAPNFKNYTRGGQISFHNLRMWDQITKTLSMICLFLWIVFTVFFAWIFIPLEKITHVLAFYYAHFLNL
VGQKHTFELSFQGKAYKQTVDSILHYTYYQDNTTQVIDWLGKSAFWAFCLSLILGLVLAYYFIKRGKAQS
DSQFVRGSQLKNSNEVRKKILRDKAHSDITIDTFPLIKNSEVQHVLVHGTVGTGKSQLIMKIMDCLRKRG
DRVIVYDKGCSFIPHYYQEDSDVILNPFDKRCANWDMWLEAPRDSDFENMAESLIPMHGESDPFWVNASR
TVFSCLGSVMRHHSDCSLEKLLKLILTDEFSALEEYLQGTPAATLVCTKIEKTAISIRATLTTYLKSFSA
LAQLNQEGEPPFSIRDYILDEQQKGWLFISSNGETHKSLKPLISMWLAQASLALLSLKPDRNRRIWFICD
ELPSLHKLPLLGETIAEVRKFGGCFLLGMQSFSQLTKVYGQAGAKEIFDLLNTRFFFRSPSSDMARLVAL
ELGEEEIEESRENYSYGANSIRDGISLGAQRVTRPIVSYPQIMELKDLHCFVRLPGHYPITQLALNLGFR
TTKTNGFIERSMPLGFNFSQLKSFDDWESSTDGHMRNKQALMNTNAEHKYIDELTEL

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(169-555)

F sex factor protein N terminal (TraD_N)

(35-126)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 46575..64528

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HBNCFIEN_RS17415 (HBNCFIEN_03512) 42175..42405 - 231 WP_182393737 hypothetical protein -
HBNCFIEN_RS17420 42456..42626 + 171 WP_182393738 hypothetical protein -
HBNCFIEN_RS17425 (HBNCFIEN_03514) 42687..42998 - 312 WP_255464431 mobilization protein -
HBNCFIEN_RS17430 (HBNCFIEN_03515) 43203..45872 + 2670 WP_182393739 Ti-type conjugative transfer relaxase TraA -
HBNCFIEN_RS17435 (HBNCFIEN_03516) 45883..46503 + 621 WP_182393740 hypothetical protein -
HBNCFIEN_RS17440 (HBNCFIEN_03517) 46575..48428 - 1854 WP_182393741 type IV conjugative transfer system coupling protein TraD virb4
HBNCFIEN_RS17445 (HBNCFIEN_03518) 48428..49219 - 792 WP_182393742 hypothetical protein -
HBNCFIEN_RS17450 (HBNCFIEN_03519) 49221..52052 - 2832 WP_182393743 conjugal transfer mating-pair stabilization protein TraG traG
HBNCFIEN_RS17455 (HBNCFIEN_03520) 52065..53429 - 1365 WP_182393744 conjugal transfer protein TraH traH
HBNCFIEN_RS17460 (HBNCFIEN_03521) 53429..53938 - 510 WP_255464432 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
HBNCFIEN_RS17465 (HBNCFIEN_03522) 53935..54681 - 747 WP_182393745 type-F conjugative transfer system pilin assembly protein TraF traF
HBNCFIEN_RS17470 (HBNCFIEN_03523) 54674..56362 - 1689 WP_182393746 conjugal transfer protein TraN traN
HBNCFIEN_RS17475 (HBNCFIEN_03524) 56355..56777 - 423 WP_182393747 type-F conjugative transfer system pilin assembly protein TrbC trbC
HBNCFIEN_RS17480 (HBNCFIEN_03525) 56790..57773 - 984 WP_182393748 conjugal transfer pilus assembly protein TraU traU
HBNCFIEN_RS17485 (HBNCFIEN_03526) 57763..58389 - 627 WP_255464438 type-F conjugative transfer system protein TraW traW
HBNCFIEN_RS17490 (HBNCFIEN_03527) 58395..58730 - 336 WP_182393750 type-F conjugative transfer system protein TrbI -
HBNCFIEN_RS17495 (HBNCFIEN_03528) 58721..61321 - 2601 WP_182393751 type IV secretion system protein TraC virb4
HBNCFIEN_RS17500 (HBNCFIEN_03529) 61312..61593 - 282 WP_182393752 conjugal transfer protein traV
HBNCFIEN_RS17505 (HBNCFIEN_03530) 61590..62960 - 1371 WP_182393753 TrbI/VirB10 family protein traB
HBNCFIEN_RS17510 (HBNCFIEN_03531) 62953..63669 - 717 WP_182393754 type-F conjugative transfer system secretin TraK traK
HBNCFIEN_RS17515 (HBNCFIEN_03532) 63662..64231 - 570 WP_182393755 type IV conjugative transfer system protein TraE traE
HBNCFIEN_RS17520 (HBNCFIEN_03533) 64241..64528 - 288 WP_255464433 type IV conjugative transfer system protein TraL traL
HBNCFIEN_RS17525 (HBNCFIEN_03534) 64549..64836 - 288 WP_182393757 hypothetical protein -
HBNCFIEN_RS17530 (HBNCFIEN_03535) 64953..65693 - 741 WP_182393758 complement resistance protein TraT -
HBNCFIEN_RS17535 (HBNCFIEN_03536) 65747..66808 - 1062 WP_182393759 replication protein -
HBNCFIEN_RS17540 (HBNCFIEN_03537) 66786..67028 - 243 WP_182393760 TraY domain-containing protein -
HBNCFIEN_RS17545 (HBNCFIEN_03538) 67219..67878 + 660 WP_182393761 S24 family peptidase -
HBNCFIEN_RS17550 (HBNCFIEN_03539) 67899..68636 + 738 WP_182393762 VUT family protein -
HBNCFIEN_RS17555 (HBNCFIEN_03540) 68605..69138 - 534 WP_182393763 hypothetical protein -

Host bacterium

ID   14884 GenBank   NZ_CP059578
Plasmid name   pPC997_2 Incompatibility group   -
Plasmid size   79080 bp Coordinate of oriT [Strand]   43100..43132 [-]
Host baterium   Legionella sp. PC997

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA1