Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 114442 |
| Name | oriT_pSER-840e  |
| Organism | Serratia sp. SSNIH1 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP026384 (30886..32591 [+], 1706 nt) |
| oriT length | 1706 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 1706 nt
>oriT_pSER-840e
GCCGTATGAATGACATCTATTTCCGTCCAATTCATCGAAACCAGTATTTTGAGGAAATGAAAGCATGATAAAGCAAATTAAAGTAATCATGTTGGCAGCAATAGTTACCGGATGTTCATCGCCGCCGCCTCCCGTTCCTGTTGAGTGGGATAAAGCCGCTGCCCCATTAAATACCAGTTTACCGCAATGGAGTGACAATAACGTTATAGTTCCTTCCCCGACAGTTAACGGTAAATGGACATCATCAATCAGCGCACAAAGTTTCTACGATACAATATGGACTCCAGCAGTATTTTACGCAATCGCCCATTCAACACGAATTGTCGTCACTGCACAATCTGGCACTGATTTCTTTAATGCCAAAAACTGGCTTCGCCAGAATGGTGCAAAAGGCGTTATTGAATACCACCCTGTCTTTAATTGCCTGACGTGCAGAGAAACCACTATTTATTTCTCTCGCCAATCCATTTAAGCAACACAATCTAATAAGGATTTATCATGAAAAAAACCGTTATCGCGGCGGCTGTATTATGCCTGACCGCAGGAACGCCGATTTATTCCTTTGCCGCTGACCCGTGCGAAGTTGTTCTTTGCATGTACGGAAAAGCTACAGGTAATAGTGGTGGGAGTGAATGCAGTTCAGCAGAACGCGCATTTTTTAAAATCAACGCATTTAAAAAACACCATCGTTTTAATCCGGGTAAAACATTCGATATGCGAAAAGAATTTTTAGGGGAGTGCAAAGCCGCAGACCCTGAATCAATAAGAAAAATCCTTTCAAAATTTGGCAGAGTGAAAGGATAACTGACATATCCATTTTGATATGAGAGGTTCGAAATGATAAACGAAGATTTATTCATTAAGAATATACACTCAAAAAATCAGGACAGAATATCTGTAGCCCTAGTATATGACACGTTATCAAAAGAAGCCCAAAGAGGTTGTGGGCTGTATTACGAAATATACGAAAGCTGTTTTATCGGTCTTTTGCGCGATCATCTTTCAGAACTTAATGAGGTTGACGCAAACAAATTAAGACGCTATGCCGAAAGCAAAGGCACAAAAATTGATGATGCGTCCTACAGTGAAGCGCTGGAAGCTGAAAGGGAGTGCAGGGCTGAAATATACCGCGAGCAGATGTAATCACACAAAACAAAAGTGTATTGTACAATACACAGACTAACAGGCGACGGGATTTTAAGGCTTCATTATTTTTGGCTTCAATTTCGAAGAAATTGTTAAGACGAAAAATAATGAAGTGGTACGCGGAAAGAAAATTCAATTTCGGAGAAATTGCTAATTTTCAGGAACCGCGCCCACCTGCGAACATTCGCCCACAGCGAATTGAGCAGGCCGGGGGGAGCCGCAAAAATTTTGCCGCCCCAGCGAGCAAAAAGGCACGCCCACGTGCCGGTTTTTGTGGGGGGCTTGCCCCGCACACCGTCGCAGCCAGGTAATATAACCCGCCCCAGCGGGGGATATTACCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCGATTTTTTCGAGCTTGCGAGGAAAAATAGGCTAAACGCAGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCAAGACGGTGTGCCGCCTTTGACCCTGGGGGTTTTGGCTCGGCGTCCAGCCGGGACATTACCCCCATGTGTCATGCAGTGAGGGCGTCCAGCCCGAACGTAATGGCACACGCCGT
GCCGTATGAATGACATCTATTTCCGTCCAATTCATCGAAACCAGTATTTTGAGGAAATGAAAGCATGATAAAGCAAATTAAAGTAATCATGTTGGCAGCAATAGTTACCGGATGTTCATCGCCGCCGCCTCCCGTTCCTGTTGAGTGGGATAAAGCCGCTGCCCCATTAAATACCAGTTTACCGCAATGGAGTGACAATAACGTTATAGTTCCTTCCCCGACAGTTAACGGTAAATGGACATCATCAATCAGCGCACAAAGTTTCTACGATACAATATGGACTCCAGCAGTATTTTACGCAATCGCCCATTCAACACGAATTGTCGTCACTGCACAATCTGGCACTGATTTCTTTAATGCCAAAAACTGGCTTCGCCAGAATGGTGCAAAAGGCGTTATTGAATACCACCCTGTCTTTAATTGCCTGACGTGCAGAGAAACCACTATTTATTTCTCTCGCCAATCCATTTAAGCAACACAATCTAATAAGGATTTATCATGAAAAAAACCGTTATCGCGGCGGCTGTATTATGCCTGACCGCAGGAACGCCGATTTATTCCTTTGCCGCTGACCCGTGCGAAGTTGTTCTTTGCATGTACGGAAAAGCTACAGGTAATAGTGGTGGGAGTGAATGCAGTTCAGCAGAACGCGCATTTTTTAAAATCAACGCATTTAAAAAACACCATCGTTTTAATCCGGGTAAAACATTCGATATGCGAAAAGAATTTTTAGGGGAGTGCAAAGCCGCAGACCCTGAATCAATAAGAAAAATCCTTTCAAAATTTGGCAGAGTGAAAGGATAACTGACATATCCATTTTGATATGAGAGGTTCGAAATGATAAACGAAGATTTATTCATTAAGAATATACACTCAAAAAATCAGGACAGAATATCTGTAGCCCTAGTATATGACACGTTATCAAAAGAAGCCCAAAGAGGTTGTGGGCTGTATTACGAAATATACGAAAGCTGTTTTATCGGTCTTTTGCGCGATCATCTTTCAGAACTTAATGAGGTTGACGCAAACAAATTAAGACGCTATGCCGAAAGCAAAGGCACAAAAATTGATGATGCGTCCTACAGTGAAGCGCTGGAAGCTGAAAGGGAGTGCAGGGCTGAAATATACCGCGAGCAGATGTAATCACACAAAACAAAAGTGTATTGTACAATACACAGACTAACAGGCGACGGGATTTTAAGGCTTCATTATTTTTGGCTTCAATTTCGAAGAAATTGTTAAGACGAAAAATAATGAAGTGGTACGCGGAAAGAAAATTCAATTTCGGAGAAATTGCTAATTTTCAGGAACCGCGCCCACCTGCGAACATTCGCCCACAGCGAATTGAGCAGGCCGGGGGGAGCCGCAAAAATTTTGCCGCCCCAGCGAGCAAAAAGGCACGCCCACGTGCCGGTTTTTGTGGGGGGCTTGCCCCGCACACCGTCGCAGCCAGGTAATATAACCCGCCCCAGCGGGGGATATTACCGATTAGGCGCGACCAACCCCTTTAAAGCAGCGTTCCGATTTTTTCGAGCTTGCGAGGAAAAATAGGCTAAACGCAGCGTCTTAAAGGGGTTGGTCGCGCGTAGCGCAAGACGGTGTGCCGCCTTTGACCCTGGGGGTTTTGGCTCGGCGTCCAGCCGGGACATTACCCCCATGTGTCATGCAGTGAGGGCGTCCAGCCCGAACGTAATGGCACACGCCGT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 10732 | GenBank | WP_045626245 |
| Name | t4cp2_C3F38_RS25485_pSER-840e |
UniProt ID | _ |
| Length | 912 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 912 a.a. Molecular weight: 103160.09 Da Isoelectric Point: 7.2988
>WP_045626245.1 MULTISPECIES: VirB3 family type IV secretion system protein [Enterobacterales]
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATVKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEENAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGMEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLLPVQFLVMKSLTRNEPMRFNLIAVWLR
AKGKPVANRLFGATTFMPVEHDDVDIKEFLDAMKLNQCATVKKYIPYSSHIHQHVVRSPKSDLYCTWELM
GTPFDCESDESLQFGTNQLHGLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFR
RNRLFLTVCYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEENAVFS
SQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGMEVKEFSEETATGMMDS
LLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVSQRLDLDVALDLLTSGKIAYGKHHFSIMV
YSPSLESLVADTNEISNALNNIGITPVPAEISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYP
GKRDKAPWGDAMALLRTPSGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYG
REDSFSPDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQLMKILC
TRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQENGLSIRLSQWAQGGEFGWV
FDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYRITSLLDGRRLVIFMDEFWKWLRDPVFKDFA
YNKLKTIRKLNGMLVVGTQSPAEIIKDDIAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGL
DPQARQYVVVKNQFKRGDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAK
AL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 10733 | GenBank | WP_048273398 |
| Name | t4cp2_C3F38_RS25550_pSER-840e |
UniProt ID | _ |
| Length | 578 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 578 a.a. Molecular weight: 65700.96 Da Isoelectric Point: 7.2144
>WP_048273398.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacterales]
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLLAGLTLPFIFHLPREERQNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKSLDNNILFRVPLWIMTIIFTVAGLIFHIAARRLLAGEIDNLKHRMIKKSKLERNT
RTDVRKVKELLPDSIEYNPLDYIDLKKGVFIGLNKDDQPQYITIKEFKTQHAAIIGTTGSGKGVTATILL
YQAILLGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKLNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLRDLFNTDFVQSLREDVPAFCGELEEIALVNSINATKGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDSVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKVDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTSTELPRATKMFQVQVKKENIELLSFEYK
QATLEKNESQEHKPSINL
MGSHLMQRLNAFADAFSFFLLWLQNSPVILSLLAGLTLPFIFHLPREERQNAPFWLKSVACVSIFFFIFG
TISPLTIQGLSYFFKSLDNNILFRVPLWIMTIIFTVAGLIFHIAARRLLAGEIDNLKHRMIKKSKLERNT
RTDVRKVKELLPDSIEYNPLDYIDLKKGVFIGLNKDDQPQYITIKEFKTQHAAIIGTTGSGKGVTATILL
YQAILLGEAVFVEDPKDDEWAPHVLREACKKAGKKFTLINLNKLNFQLDLLADISHEQLEELFNAGFSLA
KKGEASDFYRISDRRAARNTSAIYEKGMTLRDLFNTDFVQSLREDVPAFCGELEEIALVNSINATKGFSL
KEVFDEGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINSTPRTVAIFLDELKYHLSRPALEGLGT
ARDKGVHIFMAFQAIDDLRDCPSDLNGDSVIGAIIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRK
VRTDLSLTEKVDTDRMIRQAESYYVDSNMFLNLPEKVGFVFTSTELPRATKMFQVQVKKENIELLSFEYK
QATLEKNESQEHKPSINL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 21031..34544
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C3F38_RS25440 (C3F38_25435) | 16677..16958 | - | 282 | WP_000780222 | helix-turn-helix transcriptional regulator | - |
| C3F38_RS25445 (C3F38_25440) | 16939..17268 | - | 330 | WP_024154016 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| C3F38_RS25450 (C3F38_25445) | 18131..18838 | - | 708 | WP_254908225 | RepA protein | - |
| C3F38_RS25455 (C3F38_25450) | 19323..19691 | - | 369 | WP_009309975 | helix-turn-helix transcriptional regulator | - |
| C3F38_RS25460 (C3F38_25455) | 19948..20403 | + | 456 | WP_009309974 | transcription termination/antitermination NusG family protein | - |
| C3F38_RS25465 (C3F38_25460) | 20475..20690 | + | 216 | WP_004178170 | hypothetical protein | - |
| C3F38_RS25470 (C3F38_25465) | 20695..20913 | + | 219 | WP_045626248 | hypothetical protein | - |
| C3F38_RS26780 | 20910..21032 | - | 123 | WP_254908226 | hypothetical protein | - |
| C3F38_RS25475 (C3F38_25470) | 21031..21741 | + | 711 | WP_004178175 | lytic transglycosylase domain-containing protein | virB1 |
| C3F38_RS25480 (C3F38_25475) | 21738..22043 | + | 306 | WP_045626246 | TrbC/VirB2 family protein | virB2 |
| C3F38_RS25485 (C3F38_25480) | 22056..24794 | + | 2739 | WP_045626245 | VirB3 family type IV secretion system protein | virb4 |
| C3F38_RS25490 (C3F38_25485) | 24812..25513 | + | 702 | WP_045626243 | type IV secretion system protein | - |
| C3F38_RS25495 (C3F38_25490) | 25525..25749 | + | 225 | WP_053271848 | EexN family lipoprotein | - |
| C3F38_RS25500 (C3F38_25495) | 25761..26831 | + | 1071 | WP_045626242 | type IV secretion system protein | virB6 |
| C3F38_RS25505 (C3F38_25500) | 27053..27736 | + | 684 | WP_045626239 | type IV secretion system protein | virB8 |
| C3F38_RS25510 (C3F38_25505) | 27733..28641 | + | 909 | WP_086500691 | P-type conjugative transfer protein VirB9 | virB9 |
| C3F38_RS25515 (C3F38_25510) | 28688..29938 | + | 1251 | WP_045626238 | VirB10/TraB/TrbI family type IV secretion system protein | virB10 |
| C3F38_RS25520 (C3F38_25515) | 29928..30953 | + | 1026 | WP_045626236 | P-type DNA transfer ATPase VirB11 | virB11 |
| C3F38_RS25525 (C3F38_25520) | 30950..31357 | + | 408 | WP_072050230 | cag pathogenicity island Cag12 family protein | - |
| C3F38_RS25530 (C3F38_25525) | 31384..31689 | + | 306 | WP_045626234 | conjugal transfer protein | - |
| C3F38_RS25535 (C3F38_25530) | 31723..32028 | + | 306 | WP_045626232 | dopa decarboxylase remnant | - |
| C3F38_RS25550 (C3F38_25545) | 32808..34544 | + | 1737 | WP_048273398 | type IV secretory system conjugative DNA transfer family protein | virb4 |
| C3F38_RS25555 (C3F38_25550) | 34553..35308 | + | 756 | WP_023316386 | MobC family replication-relaxation protein | - |
| C3F38_RS25560 (C3F38_25555) | 35345..35818 | + | 474 | WP_045626229 | thermonuclease family protein | - |
| C3F38_RS25565 (C3F38_25560) | 35924..36220 | + | 297 | WP_086814137 | hypothetical protein | - |
| C3F38_RS25570 (C3F38_25565) | 36258..36491 | - | 234 | WP_004178129 | hypothetical protein | - |
Host bacterium
| ID | 14877 | GenBank | NZ_CP026384 |
| Plasmid name | pSER-840e | Incompatibility group | - |
| Plasmid size | 36870 bp | Coordinate of oriT [Strand] | 30886..32591 [+] |
| Host baterium | Serratia sp. SSNIH1 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |