Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   114436
Name   oriT_AR_0157|unnamed5 in_silico
Organism   Citrobacter sp. CRE-46 strain AR_0157
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP029733 (45561..45649 [+], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_AR_0157|unnamed5
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTAATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTTTTTTCGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   9256 GenBank   WP_110498925
Name   Relaxase_AN232_RS30125_AR_0157|unnamed5 insolico UniProt ID   _
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 102476.55 Da        Isoelectric Point: 8.5989

>WP_110498925.1 relaxase/mobilization nuclease domain-containing protein [Citrobacter sp. CRE-46]
MIAVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVKASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGSEWVNFYGVTCFHNCTSIESAAEEMEFSARKAKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLAGHQFVSAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSIERDRQSAWKRGRIQSLREYIADTAIAGLREAPVTDWSSLHQRFAKEGLFLSAD
NGELKVKDGWDSERPGVTLSAFGHAFDTEKLIRKLGKFTPPAQDIFSQVQAVGRYEPEKITVPSRPERMT
EKDTLTDYATARLRAPLIALDQDPSQRTIQSVHTLLAKSGLYLKEQHDRLVICDGYDPTRTPVRAERVWP
ALTKAVLDSYKGGWKPVPKDIFQQVPPAEQFTGGGLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQEIETLISSKHFSWERCHEQFAKQGLLLVREQQGLVVMDAYNREQTPVKASHVHPDLTLAR
AEPHAGSFVPVPADIFERVKPVSRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLRGRERYQAIHDECRMRKARIRIEHRDPLVRKLYYHIVELQRMQALITLKEAMKAERAELIGQGT
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYKDRRGNAVKSTTDSRCVVICEPGGTPMYSGVPGLQASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNTGDYDSLKRDMVKVAPVLFSRSPTVAMAPEGNDEQFNTAFAKM
VAWHNVNHRDEAEYHIARPDVDQLRVEHEQQFTNVLNQSSYAPDAQASHQNTWEPPTPR

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(53-317)


  Protein structure



No available structure.




T4CP

ID   10726 GenBank   WP_053389033
Name   trbC_AN232_RS30105_AR_0157|unnamed5 insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 87413.54 Da        Isoelectric Point: 6.8253

>WP_053389033.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MNNTPVNQALMKRNAWNSPVLELMRDHLLYAGIMAGSVVAGFIWPLAIPLFLLLTIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSFFRAFPSLFQYETTQESKGKGIFYIGYKRMNDIGRELWLTLDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IQNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAGLYCRAVDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHKLE
GLDSEALEITKYKGKFPYMNYLDEVGAYYTERIAVQATQVRSLEFALVMMSQDQERIENQTSAANVATLM
QNAGTKVAGKIVSDDKTARTISNAAGQEARANMVSLQRQDGLIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIEPQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAAENVSKIIG
VLTEKPSRRRKAARTEPWKIIDTFQQRLANRQEAHNLMTEYDMDHGGREENLWAEALEIIRTTTMAERTI
RYITLNKPESESTEVNAEEIQLAPDAILRNLTLPQPAYVPPARHRNEPFFSPKQTSSPQPDMEWPE

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(440-527)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 631..36372

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN232_RS29940 (AN232_29940) 128..628 + 501 WP_162620755 type IV pilus biogenesis protein PilP -
AN232_RS29945 (AN232_29945) 631..2190 + 1560 WP_048227627 GspE/PulE family protein virB11
AN232_RS29950 (AN232_29950) 2183..3277 + 1095 WP_040231337 type II secretion system F family protein -
AN232_RS29955 (AN232_29955) 3291..3905 + 615 WP_052463753 type 4 pilus major pilin -
AN232_RS29960 (AN232_29960) 3990..4472 + 483 WP_069325157 lytic transglycosylase domain-containing protein virB1
AN232_RS29965 (AN232_29965) 4457..5131 + 675 WP_048227628 prepilin peptidase -
AN232_RS29970 (AN232_29970) 5128..6414 + 1287 WP_058687259 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
AN232_RS29975 (AN232_29975) 6469..6975 + 507 WP_077223963 sugar O-acetyltransferase -
AN232_RS29985 (AN232_29985) 7858..9018 + 1161 WP_048227629 site-specific integrase -
AN232_RS31685 9038..9514 + 477 WP_162620756 hypothetical protein -
AN232_RS29990 (AN232_29990) 9660..10496 + 837 WP_048227630 hypothetical protein traE
AN232_RS29995 (AN232_29995) 10599..11807 + 1209 WP_099530103 conjugal transfer protein TraF -
AN232_RS30000 (AN232_30000) 11940..12413 + 474 WP_048227632 DotD/TraH family lipoprotein -
AN232_RS30005 (AN232_30005) 12410..13216 + 807 WP_110498920 type IV secretory system conjugative DNA transfer family protein traI
AN232_RS30010 (AN232_30010) 13228..14388 + 1161 WP_110498921 plasmid transfer ATPase TraJ virB11
AN232_RS30015 (AN232_30015) 14385..14672 + 288 WP_048227635 hypothetical protein traK
AN232_RS32275 14744..15976 + 1233 Protein_17 DUF5710 domain-containing protein -
AN232_RS32280 17558..19231 + 1674 WP_239509334 LPD7 domain-containing protein -
AN232_RS30025 (AN232_30025) 19249..19599 + 351 WP_053389043 hypothetical protein traL
AN232_RS30030 (AN232_30030) 19605..20306 + 702 WP_048227637 DotI/IcmL/TraM family protein traM
AN232_RS30035 (AN232_30035) 20317..21300 + 984 WP_048227638 DotH/IcmK family type IV secretion protein traN
AN232_RS30040 (AN232_30040) 21300..22616 + 1317 WP_099530109 conjugal transfer protein TraO traO
AN232_RS30045 (AN232_30045) 22597..23370 + 774 WP_129719564 conjugal transfer protein TraP traP
AN232_RS30050 (AN232_30050) 23370..23894 + 525 WP_048227641 conjugal transfer protein TraQ traQ
AN232_RS30055 (AN232_30055) 23929..24333 + 405 WP_048227642 DUF6750 family protein traR
AN232_RS30060 (AN232_30060) 24517..25248 + 732 WP_069325142 hypothetical protein traT
AN232_RS30065 (AN232_30065) 25320..28373 + 3054 WP_048227643 conjugal transfer protein traU
AN232_RS31690 28370..28942 + 573 WP_161556285 conjugal transfer protein TraV traV
AN232_RS30070 (AN232_30070) 28955..30157 + 1203 WP_048227644 conjugal transfer protein TraW traW
AN232_RS30075 (AN232_30075) 30154..30696 + 543 WP_094935555 conjugal transfer protein TraX -
AN232_RS30080 (AN232_30080) 30753..32915 + 2163 WP_053389037 DotA/TraY family protein traY
AN232_RS30085 (AN232_30085) 32977..33609 + 633 WP_053069310 plasmid IncI1-type surface exclusion protein ExcA -
AN232_RS30090 (AN232_30090) 33912..35120 + 1209 WP_110498923 conjugal transfer protein TrbA trbA
AN232_RS30095 (AN232_30095) 35173..36372 + 1200 WP_053389035 thioredoxin fold domain-containing protein trbB
AN232_RS30100 (AN232_30100) 36428..37558 - 1131 WP_053389034 RNA-guided endonuclease TnpB family protein -
AN232_RS30105 (AN232_30105) 37667..39967 + 2301 WP_053389033 F-type conjugative transfer protein TrbC -
AN232_RS30110 (AN232_30110) 40040..40309 + 270 WP_110498924 hypothetical protein -
AN232_RS30115 (AN232_30115) 40340..41116 - 777 WP_121572319 hypothetical protein -

Host bacterium

ID   14871 GenBank   NZ_CP029733
Plasmid name   AR_0157|unnamed5 Incompatibility group   -
Plasmid size   83094 bp Coordinate of oriT [Strand]   45561..45649 [+]
Host baterium   Citrobacter sp. CRE-46 strain AR_0157

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -